EpigenDx manufactures methylated genomic DNA controls and are required in all scientific studies. Certain types of research, such as DNA methylation tests, require specific controls. They are intended to serve as positive or negative controls in DNA methylation analysis, and are available for human, mouse, rat, and monkey DNA methylation analysis assays.
DNA Methylation is Powerful
Pyrosequencing is able to quantitate the level of a nucleotide at a designated germ-line or somatic variant, including single nucleotide polymorphisms (SNPs). The amplification signal in Pyrosequencing is proportional to the amount of genetic material and nucleotide present in the order of the sequence.
Our highly methylated DNA is in vitro enzymatically methylated genomic DNA with greater than 85% methylation. Our low methylated DNA is chemically and enzymatically treated with lower than 5% methylation. They are tested on numerous gene-specific and global methylation assays using pyrosequencing technology.
Bisulfite sequencing employing NGS technologies, methylation-specific PCR (MSP), and methylation-sensitive restriction enzyme (MSRE) assays are the most regularly utilized DNA methylation tests.
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Methylation Control Data
High Methylated Control gDNA (Mouse LINE1 Assay)
Low Methylated Control gDNA (Mouse LINE1 Assay)
Product Contents (Human/Mouse/Rat/Monkey):
HIGH Methylation Control
- 1 vial High Methylated Genomic DNA (5 µg at 100 ng/µL, > 85% Methylation)
Product Contents: HIGH Methylation Control
- 1 vial Low Methylated Genomic DNA (5 µg at 100 ng/µL, < 5% Methylation)
Pre-mixed Calibration Standard
- 1 vial 0% Methylated Genomic DNA (1 µg at 50 ng/µL)
- 1 vial 10% Methylated Genomic DNA (1 µg at 50 ng/µL)
- 1 vial 25% Methylated Genomic DNA (1 µg at 50 ng/µL)
- 1 vial 50% Methylated Genomic DNA (1 µg at 50 ng/µL)
- 1 vial 75% Methylated Genomic DNA (1 µg at 50 ng/µL)
- 1 vial 90% Methylated Genomic DNA (1 µg at 50 ng/µL)
How It Works
Tell Us About Your Project
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Experts Since 2006
With over 15 years of experience, EpigenDx offers customizable DNA Methylation analysis for specific gene targets of your interest. We offer rapid turnaround time for clinical trials and molecular diagnosis as well as MGMT/Line-1/Foxp3 tests for HRM realtime PCR, Pyrosequencing for SNP genotyping, and targeted bisulfite NGS. Simply send us custom target regions with your genes of interest. We will design and validate a panel of assays suited to your research interests.
Experts in DNA Methylation