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WORLD-LEADING DNA METHYLATION EXPERTISE

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Human

NGS162 – Human ANO1 (anoctamin 1)
PCR ID Gene Alias NCBI ID Assay Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N162P1 ANO1 TMEM16A 55107 ASY3379 Intron 9 46019 to 46108 46324 to 46413 Chr11:70124625-70124714 5 171 0.612 800
5-plex ANO1 TMEM16A 55107 ASY3389 5′-Upstream  -2559 to -2491  -2254 to -2186 Chr11:70076048-70076116 2 152 0.909 200
Ta = 65 ANO1 TMEM16A 55107 ASY3316 Intron 1 556 to 642 861 to 947 Chr11:70079162-70079248 4 142 0.856 600
ANO1 TMEM16A 55107 ASY3310 5′-Upstream  -735 to -667  -430 to -362 Chr11:70077872-70077940 6 138 0.946 3100
ANO1 TMEM16A 55107 ASY3315 Intron 1 445 to 484 750 to 789 Chr11:70079051-70079090 4 97 0.729 1200
N162P2 ANO1 TMEM16A 55107 ASY3378 Exon 6, Intron 6 29769 to 29885 30074 to 30190 Chr11:70108375-70108491 6 168 0.851 1300
9-plex ANO1 TMEM16A 55107 ASY3317 Intron 1 689 to 759 994 to 1064 Chr11:70079295-70079365 3 157 0.877 700
Ta = 56 ANO1 TMEM16A 55107 ASY3309 5′-Upstream  -840 to -771  -535 to -466 Chr11:70077767-70077836 6 123 0.832 2200
ANO1 TMEM16A 55107 ASY3381 Intron 12 71493 to 71528 71798 to 71833 Chr11:70150099-70150134 4 117 0.874 400
ANO1 TMEM16A 55107 ASY2500 Intron 15 77571 to 77621 77876 to 77926 Chr11:70156177-70156227 5 114 0.857 500
ANO1 TMEM16A 55107 ADS9064 Intron 2 22409 to 22475 22714 to 22780 Chr11:70101015-70101081 4 113 0.692 2000
ANO1 TMEM16A 55107 ASY3375 Intron 2 11279 to 11331 11584 to 11636 Chr11:70089885-70089937 4 104 0.846 400
ANO1 TMEM16A 55107 ASY3374 Intron 2 10351 to 10384 10656 to 10689 Chr11:70088957-70088990 4 97 0.831 1600
ANO1 TMEM16A 55107 ASY3313 5′-UTR  -300 to -254 6 to 52 Chr11:70078307-70078353 8 95 0.896 600
N162P3 ANO1 TMEM16A 55107 ASY3380 Intron 11 62559 to 62753 62864 to 63058 Chr11:70141165-70141359 11 243 0.786 100
5-plex ANO1 TMEM16A 55107 ASY3390 5′-Upstream  -1906 to -1817  -1601 to -1512 Chr11:70076701-70076790 3 199 0.890 100
Ta = 59 ANO1 TMEM16A 55107 ASY3382 Intron 16 79636 to 79771 79941 to 80076 Chr11:70158242-70158377 7 192 0.709 500
ANO1 TMEM16A 55107 ASY3376 Intron 2 12418 to 12548 12723 to 12853 Chr11:70091024-70091154 6 191 0.810 1100
ANO1 TMEM16A 55107 ASY3377 Intron 3 25230 to 25290 25535 to 25595 Chr11:70103836-70103896 5 109 0.637 300
3 PCRs 1 gene 19 assays 97 CpGs 2,722 bp

NGS158 – Human ST3GAL4 (ST3 beta-galactoside alpha-2,3-sialyltransferase 4)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N158P1 ST3GAL4 STZ 6484 ASY3333 Intron 1 -13841 to -13782 36630 to 36689 Chr11:126392315-126392374 2 145 0.893 100
4-plex ST3GAL4 STZ 6484 ASY3328 Intron 1 -49904 to -49872 567 to 599 Chr11:126356252-126356284 4 92 0.904 1,400
Ta = 62 ST3GAL4 STZ 6484 ASY3334 Intron 1 -520 49951 Chr11:126405636 1 90 0.911 200
ST3GAL4 STZ 6484 ASY3326 5′-Upstream -51078 to -51066 -608 to -596 Chr11:126355078-126355090 2 69 0.835 300
N158P2 ST3GAL4 STZ 6484 ASY3331 Intron 1 -39417 to -39288 11054 to 11183 Chr11:126366739-126366868 6 192 0.860 500
5-plex ST3GAL4 STZ 6484 ASY3330 Intron 1 -49285 to -49254 1186 to 1217 Chr11:126356871-126356902 3 130 0.717 100
Ta = 59 ST3GAL4 STZ 6484 ASY3338 Intron 9 7022 to 7067 57492 to 57537 Chr11:126413177-126413222 3 120 0.909 200
ST3GAL4 STZ 6484 ASY3336 Intron 1, 5′-UTR -87 to -45 50384 to 50426 Chr11:126406069-126406111 3 112 0.862 1,300
ST3GAL4 STZ 6484 ASY3337 Intron 9 6268 to 6310 56738 to 56780 Chr11:126412423-126412465 3 110 0.917 1,300
N158P3 ST3GAL4 STZ 6484 ASY3325 5′-Upstream -51393 to -51355 -923 to -885 Chr11:126354763-126354801 2 195 0.887 100
3-plex ST3GAL4 STZ 6484 ASY3339 Exon 11 8072 to 8131 58542 to 58601 Chr11:126414227-126414286 5 149 0.907 600
Ta = 53 ST3GAL4 STZ 6484 ASY3327 5′-Upstream -50846 to -50754 -376 to -284 Chr11:126355310-126355402 7 143 0.854 200
3 PCRs 1 gene 12 assays 41 CpGs 1,547 bp
NGS153 – Human FUT7 (fucosyltransferase 7)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N153P1 FUT7 FucT-VII 2529 ASY3096 5′-Upstream -2717 to -2665 -2620 to -2568 Chr9:137034708-137034656 5 121 0.426 500
8-plex FUT7 FucT-VII 2529 ASY3034 5′-Upstream -1083 to -1051 -986 to -954 Chr9:137033074-137033042 2 83 0.318 300
Ta = 59 FUT7 FucT-VII 2529 ASY3103 5′-Upstream -518 to -468 -421 to -371 Chr9:137032509-137032459 3 95 0.341 300
FUT7 FucT-VII 2529 ASY3098 Intron 1 30 127 Chr9:137031962 1 87 0.385 1000
FUT7 FucT-VII 2529 ASY3067 Exon 2 637 to 716 734 to 813 Chr9:137031355-137031276 7 137 0.461 50
FUT7 FucT-VII 2529 ASY3101 3′-UTR 1287 to 1379 1384 to 1476 Chr9:137030705-137030613 7 153 0.373 300
FUT7 FucT-VII 2529 ASY3069 3′-UTR 1471 to 1509 1568 to 1606 Chr9:137030521-137030483 4 100 0.444 200
FUT7 FucT-VII 2529 ASY3088 3′-Downstream 3331 to 3374 3428 to 3471 Chr9:137028661-137028618 8 121 0.509 200
N153P2 FUT7 FucT-VII 2529 ASY3029 5′-Upstream -3685 to -3672 -3588 to -3575 Chr9:137035676-137035663 2 75 0.333 1200
8-plex FUT7 FucT-VII 2529 ASY3030 5′-Upstream -3453 to -3406 -3356 to -3309 Chr9:137035444-137035397 5 143 0.389 100
Ta = 59 FUT7 FucT-VII 2529 ASY3094 5′-Upstream -3183 to -3080 -3086 to -2983 Chr9:137035174-137035071 5 160 0.284 100
FUT7 FucT-VII 2529 ASY3095 5′-Upstream -2795 to -2785 -2698 to -2688 Chr9:137034786-137034776 2 71 0.296 600
FUT7 FucT-VII 2529 ASY3068 Exon 2 1028 to 1066 1125 to 1163 Chr9:137030964-137030926 5 100 0.410 1400
FUT7 FucT-VII 2529 ASY3071 3′-Downstream 2349 to 2396 2446 to 2493 Chr9:137029643-137029596 4 123 0.421 700
FUT7 FucT-VII 2529 ASY3072 3′-Downstream 2876 to 2961 2973 to 3058 Chr9:137029116-137029031 5 138 0.455 100
FUT7 FucT-VII 2529 ASY3093 3′-Downstream 5914 to 5957 6011 to 6054 Chr9:137026078-137026035 4 112 0.457 500
N153P3 FUT7 FucT-VII 2529 ASY3027 5′-Upstream -4025 to -4019 -3928 to -3922 Chr9:137036016-137036010 2 110 0.297 500
7-plex FUT7 FucT-VII 2529 ASY3031 5′-Upstream -3335 to -3271 -3238 to -3174 Chr9:137035326-137035262 4 126 0.241 300
Ta = 59 FUT7 FucT-VII 2529 ASY3097 5′-Upstream -2602 to -2509 -2505 to -2412 Chr9:137034593-137034500 4 152 0.212 200
FUT7 FucT-VII 2529 ASY3035 5′-Upstream -828 to -739 -731 to -642 Chr9:137032819-137032730 5 153 0.256 300
FUT7 FucT-VII 2529 ASY3065 5′-Upstream, 5′-UTR -104 to -36 -7 to 62 Chr9:137032095-137032027 5 134 0.292 100
FUT7 FucT-VII 2529 ASY3090 3′-Downstream 4630 to 4714 4727 to 4811 Chr9:137027362-137027278 3 145 0.298 100
FUT7 FucT-VII 2529 ASY3091 3′-Downstream 4909 to 4938 5006 to 5035 Chr9:137027083-137027054 3 130 0.324 100
N153P4 FUT7 FucT-VII 2529 ASY3036 5′-Upstream -298 to -225 -201 to -128 Chr9:137032289-137032216 2 138 0.577 300
3-plex FUT7 FucT-VII 2529 ASY3089 3′-Downstream 4430 to 4472 4527 to 4569 Chr9:137027562-137027520 3 105 0.234 50
Ta = 59 FUT7 FucT-VII 2529 ASY3092 3′-Downstream 5662 5759 Chr9:137026330 1 115 0.529 400
4 PCRs 1 gene 26 assays 101 CpGs 3,127 bp
NGS151 – Human CEBPA (CCAAT enhancer binding protein alpha)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N151P1 CEBPA C/EBP-α 1050 ASY2946 5′-Upstream -834 to -707 -714 to -587 Chr19:33303248-33303121 20 196 0.880 1,200
7-plex CEBPA C/EBP-α 1050 ASY2949 Exon 1, 3′-UTR 1071 to 1193 1191 to 1313 Chr19:33301344-33301222 15 175 0.989 100
Ta = 56 CEBPA C/EBP-α 1050 ASY2950 3′-UTR 1950 to 2021 2070 to 2141 Chr19:33300465-33300394 4 171 0.785 400
CEBPA C/EBP-α 1050 ASY2951 5′-Upstream -3603 to -3547 -3483 to -3427 Chr19:33306017-33305961 2 170 0.995 1,200
CEBPA C/EBP-α 1050 ASY2944 5′-Upstream, 5′-UTR -136 to -21 -16 to 100 Chr19:33302550-33302435 23 166 0.907 700
CEBPA C/EBP-α 1050 ASY2942 5′-Upstream -1371 to -1264 -1251 to -1144 Chr19:33303785-33303678 13 156 0.884 1,000
CEBPA C/EBP-α 1050 ASY2940 5′-Upstream -1933 to -1891 -1813 to -1771 Chr19:33304347-33304305 4 127 0.870 8,000
N151P2 CEBPA C/EBP-α 1050 ASY2947 Exon 1 68 to 256 188 to 376 Chr19:33302347-33302159 28 243 0.863 100
5-plex CEBPA C/EBP-α 1050 ASY2952 5′-Upstream -3291 to -3189 -3171 to -3069 Chr19:33305705-33305603 4 226 0.992 100
Ta = 59 CEBPA C/EBP-α 1050 ASY2948 Exon 1 521 to 639 641 to 759 Chr19:33301894-33301776 20 172 0.948 300
CEBPA C/EBP-α 1050 ASY2945 5′-Upstream -668 to -567 -548 to -447 Chr19:33303082-33302981 14 159 0.920 500
CEBPA C/EBP-α 1050 ASY2941 5′-Upstream -1521 to -1427 -1401 to -1307 Chr19:33303935-33303841 12 146 0.946 1,500
2 PCRs 1 gene 12 assays 159 CpGs 2,107 bp

NGS149 – Human MAD1L1 (mitotic arrest deficient 1 like 1)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N149P1 MAD1L1 MAD1 8379 ASY2875 5′-Upstream -4681 to -4571 -1571 to -1461 Chr7:2234814-2234704 4 186 0.800 500
7-plex MAD1L1 MAD1 8379 ASY2863 Intron 4, Exon 5 7327 to 7442 10437 to 10552 Chr7:2222807-2222692 12 175 0.821 3,500
Ta = 62 MAD1L1 MAD1 8379 ASY2882 Intron 18 410716 to 410820 413826 to 413930 Chr7:1819418-1819314 9 166 0.815 3,000
MAD1L1 MAD1 8379 ASY2868 Intron 11 118435 to 118509 121545 to 1221619 Chr7:2111699-2111625 5 150 0.709 1,000
MAD1L1 MAD1 8379 ASY2870 Intron 12 183124 to 183213 186234 to 186323 Chr7:2047010-2046921 6 144 0.899 4,000
MAD1L1 MAD1 8379 ASY2879 Intron 11 82533 to 82600 85643 to 85710 Chr7:2147601-2147534 5 132 0.780 600
MAD1L1 MAD1 8379 ASY2883 Intron 18 411020 to 411088 414130 to 414198 Chr7:1819114-1819046 6 121 0.931 5,000
N149P2 MAD1L1 MAD1 8379 ASY2871 Intron 16 288867 to 288979 291977 to 292089 Chr7:1941267-1941155 11 247 0.515 100
6-plex MAD1L1 MAD1 8379 ASY2867 Intron 1 -2342 to -2217 769 to 894 Chr7:2232475-2232350 10 173 0.679 1,500
Ta = 59 MAD1L1 MAD1 8379 ADS4001 Intron 17 307865 to 307951 310975 to 311061 Chr7:1922269-1922183 11 149 0.779 10,000
MAD1L1 MAD1 8379 ASY2869 Intron 14 229504 to 229579 232614 to 232689 Chr7:2000630-2000555 7 144 0.540 7,000
MAD1L1 MAD1 8379 ASY2865 5′-Upstream -3346 to -3316 -236 to -206 Chr7:2233479-2233449 2 118 0.763 5,000
MAD1L1 MAD1 8379 ASY2873 Exon 4, Intron 4 4721 to 4748 7831 to 7858 Chr7:2225413-2225386 4 93 0.740 3,500
N149P3 MAD1L1 MAD1 8379 ASY2884 Intron 12 169521 to 169538 172631 to 172648 Chr7:2060613-2060596 3 210 0.664 50
5-plex MAD1L1 MAD1 8379 ASY2880 Intron 11 126261 to 126348 129371 to 129458 Chr7:2103873-2103786 4 166 – 100
Ta = 56 MAD1L1 MAD1 8379 ASY1554 Intron 14 242401 to 242485 245511 to 245595 Chr7:1987733-1987649 13 152 0.655 100
MAD1L1 MAD1 8379 ASY2872 Intron 16 289638 to 289720 292748 to 292830 Chr7:1940496-1940414 5 148 0.697 100
MAD1L1 MAD1 8379 ASY2881 Intron 12 183428 to 183508 186538 to 186618 Chr7:2046706-2046626 7 141 0.890 3,000
MAD1L1 MAD1 8379 ASY2922 Exon 5 7476 to 7524 10586 to 10634 Chr7:2222658-2222610 2 104 0.856 10,000
3 PCRs 1 gene 19 assays 126 CpGs 2,919 bp

NGS148 – Human CDKN1A (cyclin dependent kinase inhibitor 1A)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N148P1 CDKN1A p21 1026 ASY2843 5′-Upstream -5653 to -5524 -209 to -80 Chr6:36678449-36678578 11 216 0.883 50
6-plex CDKN1A p21 1026 ASY2848 Intron 1 -582 to -527 4863 to 4918 Chr6:36683520-36683575 3 146 0.926 950
Ta = 59 CDKN1A p21 1026 ASY2839 5′-Upstream -6544 -1100 Chr6:36677558 1 142 0.954 400
CDKN1A p21 1026 ASY2844 5′-UTR -5371 to -5320 74 to 125 Chr6:36678731-36678782 7 101 0.939 2,300
CDKN1A p21 1026 ASY2846 5′-Upstream -6928 to -6882 -1484 to -1438 Chr6:36677174-36677220 2 93 0.943 1,500
CDKN1A p21 1026 ASY2838 5′-Upstream -6791 to -6779 -1347 to -1335 Chr6:36677311-36677323 2 89 0.925 1,200
N148P2 CDKN1A p21 1026 ADS1253-1 5′-Upstream, 5′-UTR -5489 to -5406 -45 to 39 Chr6:36678613-36678696 15 152 0.931 120
4-plex CDKN1A p21 1026 ASY2842 5′-Upstream -5846 to -5752 -402 to -308 Chr6:36678256-36678350 2 149 0.918 1,800
Ta = 59 CDKN1A p21 1026 ASY2837 5′-Upstream -6977 -1533 Chr6:36677125 1 117 0.942 1,800
H2BC1 TSH2B 255626 ASY2850 5′-UTR, Exon 1 -30 to 5 103 to 137 Chr6:25726879-25726913 3 88 0.960 2,800
N148P3 CDKN1A p21 1026 ASY2845 Intron 1 -5271 to -5057 174 to 388 Chr6:36678831-36679045 31 290 0.924 160
5-plex CDKN1A p21 1026 ASY2840 5′-Upstream -6379 to -6231 -935 to -787 Chr6:36677723-36677871 3 226 0.937 100
Ta = 59 H2BC1 TSH2B 255626 ASY2849 5′-Upstream, 5′-UTR -273 to -118 -141 to 15 Chr6:25726636-25726791 11 222 0.939 1,600
CDKN1A p21 1026 ASY2841 5′-Upstream -6067 to -5993 -623 to -549 Chr6:36678035-36678109 2 163 0.923 150
CDKN1A p21 1026 ASY2847 Intron 1 -4871 to -4769 574 to 676 Chr6:36679231-36679333 8 150 0.976 350
3 PCRs 2 genes 15 assays 102 CpGs 2,344 bp

NGS144/139/116 – Human Mitochondrial Fusion
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N144P1 POLRMT MTRPOL 5442 ASY2704 Exon 3 3615 to 3728 3640 to 3753 Chr19:629898-629785 8 171 0.885 1,200
10-plex RAF1 CRAF 5894 ASY2732 5′-Upstream -45667 to -45553 -271 to -157 Chr3:12664388-12664274 12 164 0.857 300
Ta = 61 MTIF3 IF3MT 219402 ASY2710 Intron 2 -8979 to -8876 1138 to 1241 Chr13:27449427-27449324 4 158 0.930 200
MFN2 CMT2A 9927 ASY2682 5′-Upstream -9514 to -9418 -657 to -561 Chr1:11979655-11979751 6 155 0.882 500
FIS1 TTC11 51024 ASY2722 5′-UTR, Intron 1 -16 to 59 62 to 136 Chr7:101245020-101244946 5 139 0.884 1,800
PDPK1 PDK1 5170 ASY2740 Intron 11 55588 to 55640 55680 to 55732 Chr16:2593700-2593752 3 132 0.944 200
FIS1 TTC11 51024 ASY2721 5′-Upstream -203 to -147 -126 to -70 Chr7:101245207-101245151 10 122 0.872 800
TFAM MTTF1 7019 ASY2688 5′-UTR, Exon 1 -39 to 27 100 to 165 Chr10:58385509-58385574 7 122 0.989 400
RAF1 CRAF 5894 ASY2734 Intron 1 -44469 to -44413 928 to 984 Chr3:12663190-12663134 8 106 0.970 1,000
OPA1 MGM1 4976 ASY2680 3′-Downstream 108532 to 108552 108724 to 108744 Chr3:193701909-193701929 4 95 0.998 250
N144P2 PDPK1 PDK1 5170 ASY2738 Intron 1 333 to 442 425 to 534 Chr16:2538445-2538554 9 163 0.884 250
8-plex PRKN PARK2 5071 ASY2749 Intron 1 963 to 967 1061 to 1065 Chr6:162726706-162726702 2 157 0.996 1,000
Ta = 61 TFB2M HKP1 64216 ASY2694 5′-Upstream, 5′-UTR -146 to -62 -23 to 62 Chr1:246566284-246566200 8 152 0.975 200
APC DP2.5 324 ASY2745 5′-UTR -166 to -83 31 to 114 Chr5:112707552-112707635 10 145 0.989 300
DNM1L DRP1 10059 ASY2713 Exon 1 8 to 87 71 to 150 Chr12:32679371-32679450 7 135 0.996 250
FIS1 TTC11 51024 ASY2723 Intron 1 535 to 604 612 to 681 Chr7:101244470-101244401 8 121 0.904 3,500
PTEN MMAC1 5728 ASY2754 Intron 1 3601 to 3628 4446 to 4473 Chr10:87868070-87868097 2 110 0.980 100
TFAM MTTF1 7019 ASY2689 Exon 1, Intron 1 69 to 109 207 to 247 Chr10:58385616-58385656 4 106 0.982 250
N144P3 TFAM MTTF1 7019 ASY2687 5′-Upstream -454 to -333 -316 to -195 Chr10:58385094-58385215 15 173 0.980 600
7-plex RAF1 CRAF 5894 ASY2731 5′-Upstream -45826 to -45739 -430 to -343 Chr3:12664547-12664460 7 148 0.939 9,000
Ta = 59 PDPK1 PDK1 5170 ASY2739 Intron 1 1607 to 1692 1699 to 1784 Chr16:2539719-2539804 5 142 0.908 2,200
POLRMT MTRPOL 5442 ASY2705 Exon 10 11878 to 11951 11903 to 11976 Chr19:621635-621562 11 132 0.896 2,000
MFN2 CMT2A 9927 ASY2685 Intron 9, Exon 10 12547 to 12611 21404 to 21468 Chr1:12001715-12001779 3 124 0.930 100
NAMPT PBEF1 10135 ASY2728 5′-Upstream -693 to -634 -594 to -535 Chr7:106285577-106285518 5 106 0.967 10,000
PRKN PARK2 5071 ASY2748 5′-Upstream -657 to -621 -559 to -523 Chr6:162728325-162728289 3 92 0.962 100
N144P4 NFE2L2 NRF2 4780 ADS3188 5′-Upstream -844 to -736 -693 to -585 Chr2:177265420-177265312 8 183 0.924 100
8-plex MTERF4 MTERFD2 130916 ASY2718 Exon 3 4894 to 4996 4916 to 5018 Chr2:241097380-241097278 4 168 0.977 150
Ta = 56 APC DP2.5 324 ASY2743 Intron 1 30193 to 30287 30389 to 30483 Chr5:112737910-112738004 5 161 0.898 200
RAF1 CRAF 5894 ASY2733 Intron 1 -45077 to -44978 320 to 419 Chr3:12663798-12663699 14 152 0.960 1,800
GABPA E4TF1A 2551 ASY2700 Intron 1 -5238 to -5174 1792 to 1856 Chr21:25736361-25736425 5 148 0.937 100
FIS1 TTC11 51024 ASY2724 Exon 2, Intron 2 974 to 1058 1051 to 1135 Chr7:101244031-101243947 7 142 0.844 100
MTIF3 IF3MT 219402 ASY2707 5′-Upstream -10337 to -10288 -221 to -172 Chr13:27450785-27450736 5 110 0.863 400
OPA1 MGM1 4976 ASY2679 Intron 28 94811 to 94833 95003 to 95025 Chr3:193688188-193688210 3 103 0.987 2,500
N144P5 APC DP2.5 324 ASY516 Intron 1 29984 to 30130 30180 to 30326 Chr5:112737701-112737847 16 194 0.989 1,200
10-plex APC DP2.5 324 ASY2744 Intron 1 30544 to 30676 30740 to 30872 Chr5:112738261-112738393 4 191 0.984 150
Ta = 56 PRKN PARK2 5071 ADS5123 5′-Upstream -379 to -263 -281 to -165 Chr6:162728047-162727931 10 174 0.990 900
OPA1 MGM1 4976 ASY2681 5′-UTR, Exon 1 -76 to 21 117 to 213 Chr3:193593302-193593398 16 152 0.989 200
PDPK1 PDK1 5170 ASY2737 5′-UTR -79 to -8 14 to 85 Chr16:2538034-2538105 15 132 0.722 1,200
MFN2 CMT2A 9927 ASY2684 Intron 2 -5633 to -5571 3225 to 3287 Chr1:11983536-11983598 2 123 0.851 2,000
DNM1L DRP1 10059 ASY2711 5′-Upstream -929 to -862 -866 to -799 Chr12:32678435-32678502 4 122 0.886 150
MTIF3 IF3MT 219402 ASY2708 Intron 1 -9952 to -9888 165 to 229 Chr13:27450400-27450336 9 115 0.871 750
TFAM MTTF1 7019 ASY2690 Intron 4 3699 3837 Chr10:58389246 1 95 0.994 400
NFE2L2 NRF2 4780 ADS8779 5′-Upstream -1493 -1342 Chr2:177266069 1 85 0.929 250
N144P6 PTEN MMAC1 5728 ADS3543 5′-Upstream -2487 to -2405 -1642 to -1560 Chr10:87861983-87862065 8 172 0.986 2,000
9-plex MFN2 CMT2A 9927 ASY2683 5′-Upstream -9112 to -9004 -255 to -147 Chr1:11980057-11980165 12 163 0.757 300
Ta = 56 MTERF4 MTERFD2 130916 ASY2720 3′-UTR 13751 to 13852 13773 to 13874 Chr2:241088523-241088422 6 154 0.979 400
OPA1 MGM1 4976 ASY2678 Intron 1 12627 12819 Chr3:193606004 1 138 0.994 1,500
GABPA E4TF1A 2551 ADS3958re 5′-Upstream -7266 to -7179 -237 to -150 Chr21:25734333-25734420 4 138 0.944 100
MTERF4 MTERFD2 130916 ASY2719 Intron 5 8262 to 8316 8284 to 8338 Chr2:241094012-241093958 2 119 0.894 1,500
APC DP2.5 324 ASY2746 5′-Upstream -335 to -278 -139 to -82 Chr5:112707383-112707440 7 108 0.990 3,000
TFB2M HKP1 64216 ASY2695 Exon 1 217 to 254 340 to 377 Chr1:246565922-246565885 5 102 0.940 3,200
RAF1 CRAF 5894 ASY2735 Intron 1 -41557 3840 Chr3:12660278 1 90 0.997 150
N139P1 PPARGC1A PGC1A 10891 ASY1844 Intron 1 3797 to 3927 3887 to 4017 Chr4:23886161-23886031 3 205 0.996 3,500
6-plex DOC2B DOC2BL 8447 ASY1835 Intron 1 1475 to 1593 1646 to 1764 Chr17:180005-179887 4 195 0.502 1,200
Ta = 62 MFN1 HFZO1 55669 ASY1850 Intron 1 -894 to -761 250 to 383 Chr3:179347958-179348091 18 182 0.980 1,000
LIPT1 LIPT1D 51601 ASY2567 Intron 2 -2186 to -2137 4818 to 4867 Chr2:99159772-99159821 3 167 0.988 500
LIPT1 LIPT1D 51601 ASY2563 5′-Upstream -7311 to -7227 -308 to -224 Chr2:99154647-99154731 8 151 0.982 2,000
DOC2B DOC2BL 8447 ASY2578 Intron 3 17831 to 17851 18002 to 18022 Chr17:163649-163629 4 113 0.557 500
N139P2 DOC2B DOC2BL 8447 ASY2572 Intron 1 2315 to 2421 2486 to 2592 Chr17:179165-179059 5 181 0.542 200
10-plex STOML2 SLP2 30968 ASY2588 Exon 7 1828 to 1923 1865 to 1960 Chr9:35101267-35101172 6 171 0.923 400
Ta = 59 PPARGC1A PGC1A 10891 ASY1840 5′-Upstream -961 to -903 -871 to -813 Chr4:23890918-23890860 3 149 0.989 3,000
STOML2 SLP2 30968 ASY2587 Exon 6 1601 to 1643 1638 to 1680 Chr9:35101494-35101452 3 140 0.932 3,000
PPARGC1A PGC1A 10891 ASY1843 Intron 1 1326 to 1374 1416 to 1464 Chr4:23888632-23888584 2 134 0.972 150
DOC2B DOC2BL 8447 ASY2568 5′-Upstream -1234 to -1176 -1063 to -1005 Chr17:182713-182655 3 131 0.660 400
STOML2 SLP2 30968 ASY2586 Exon 5 1316 to 1383 1353 to 1420 Chr9:35101779-35101712 3 128 0.905 900
LIPT1 LIPT1D 51601 ASY2562 5′-Upstream -7410 to -7356 -407 to -353 Chr2:99154548-99154602 3 122 0.995 150
STOML2 SLP2 30968 ASY2580 5′-Upstream -543 to -500 -506 to -463 Chr9:35103637-35103594 3 103 0.873 150
DOC2B DOC2BL 8447 ASY2571 5′-Upstream -652 to -650 -481 to -479 Chr17:182131-182129 2 102 0.720 1,000
N139P3 DOC2B DOC2BL 8447 ASY2569 5′-Upstream -1130 to -997 -959 to -826 Chr17:182609-182476 5 204 0.672 100
11-plex PPARGC1A PGC1A 10891 ASY1847 Intron 12 92946 to 93055 93036 to 93145 Chr4:23797012-23796903 3 195 0.991 700
Ta = 56 STOML2 SLP2 30968 ASY2583 5′-Upstream, 5′-UTR, Exon 1 -127 to 19 -90 to 56 Chr9:35103221-35103076 20 190 0.988 1,500
PPARGC1A PGC1A 10891 ADS2164 5′-UTR, Intron 1 -17 to 94 74 to 184 Chr4:23889974-23889864 6 174 0.998 400
LIPT1 LIPT1D 51601 ASY2561 5′-Upstream -7527 to -7464 -524 to -461 Chr2:99154431-99154494 3 168 0.975 200
LIPT1 LIPT1D 51601 ASY2566 Intron 1 -6543 to -6437 461 to 567 Chr2:99155415-99155521 5 168 0.960 8,000
DOC2B DOC2BL 8447 ASY2573 Intron 1 2561 to 2635 2732 to 2806 Chr17:178919-178845 2 150 0.617 400
LIPT1 LIPT1D 51601 ASY1833 Exon 3 507 to 560 7510 to 7563 Chr2:99162464-99162517 3 138 0.993 3,000
STOML2 SLP2 30968 ASY2585 Intron 3, Exon 4 1110 to 1171 1147 to 1208 Chr9:35101985-35101924 3 122 0.922 1,000
STOML2 SLP2 30968 ASY2582 Intron 2 461 to 514 498 to 551 Chr9:35102634-35102581 4 121 0.921 1,500
LIPT1 LIPT1D 51601 ASY2564 5′-Upstream -7190 to -7140 -187 to -137 Chr2:99154768-99154818 5 119 0.989 1,500
N139P4 STOML2 SLP2 30968 ASY2579 5′-Upstream -455 to -316 -418 to -279 Chr9:35103549-35103410 8 186 0.871 6,000
8-plex DOC2B DOC2BL 8447 ASY2570 5′-Upstream -950 to -854 -779 to -683 Chr17:182429-182333 4 158 0.616 1,300
Ta = 59 MFN1 HFZO1 55669 ASY1849 5′-Upstream -1293 to -1196 -150 to -53 Chr3:179347559-179347656 5 158 0.947 2,500
LIPT1 LIPT1D 51601 ASY2565 Intron 1 -6680 to -6605 324 to 399 Chr2:99155278-99155353 6 141 0.995 4,500
STOML2 SLP2 30968 ASY2584 Exon 3 931 to 999 968 to 1036 Chr9:35102164-35102096 5 130 0.934 5,000
PPARGC1A PGC1A 10891 ASY1838 5′-Upstream -1042 -952 Chr4:23890999 1 106 0.992 100
DOC2B DOC2BL 8447 ASY1834 5′-Upstream -2358 to -2338 -2187 to -2167 Chr17:183837-183817 2 103 0.501 400
PPARGC1A PGC1A 10891 ASY1837 5′-Upstream -1310 -1220 Chr4:23891267 1 97 0.992 500
N139P5 MFN1 HFZO1 55669 ASY1852 Intron 9, Exon 10 26326 to 26404 27469 to 27547 Chr3:179375177-179375255 5 208 0.962 200
6-plex MFN1 HFZO1 55669 ASY1851 Intron 10 27364 to 27437 28507 to 28580 Chr3:179376215-179376288 3 189 0.937 4,500
Ta = 56 STOML2 SLP2 30968 ADS5520 5′-Upstream -293 to -150 -256 to -113 Chr9:35103387-35103244 12 186 0.872 1,000
DOC2B DOC2BL 8447 ASY2577 Intron 2 16501 to 16562 16672 to 16733 Chr17:164979-164918 4 172 0.650 700
PPARGC1A PGC1A 10891 ASY1841 Intron 1 604 to 699 694 to 789 Chr4:23889354-23889259 8 165 0.991 300
PPARGC1A PGC1A 10891 ASY1842 Intron 1 879 to 965 969 to 1055 Chr4:23889079-23888993 6 141 0.989 200
N139P6 PPARGC1A PGC1A 10891 ASY1845 Intron 2 14068 to 14292 14158 to 14382 Chr4:23875890-23875666 4 275 0.984 300
3-plex PPARGC1A PGC1A 10891 ASY1848 3′-UTR 94956 to 95127 95046 to 95217 Chr4:23795002-23794831 5 237 0.982 100
Ta = 62 STOML2 SLP2 30968 ASY2581 Intron 1 53 to 113 90 to 150 Chr9:35103042-35102982 3 118 0.947 2,500
N116P1 UQCRC2 QCR2 7385 ADS9486 5′-UTR, Intron 1 -88 to 48 677 to 812 Chr16:21953336-21953471 9 189 0.991 100
9-plex PCDH9 n/a 5101 ADS9490 5′-UTR, Intron 1 -1340 to -1251 666 to 755 Chr13:67229780-67229691 4 150 0.968 500
Ta = 59 PCCB n/a 5096 ADS9481 Intron 1 670 to 769 740 to 839 Chr3:136251045-136251144 7 149 0.996 400
PCDHA12 PCDHα12 56137 ADS9492 Intron 1 7177 to 7270 7177 to 7270 Chr5:140882649-140882742 7 142 0.954 100
RIMS3 NIM3 9783 ADS9493 5′-Upstream -27205 -3473 Chr1:40669130 1 142 0.982 1,000
PCDHA12 PCDHα12 56137 ADS9491 Exon 1 327 to 392 327 to 392 Chr5:140875799-140875864 6 124 0.836 250
ASTN1 ASTN 460 ADS9470 Intron 16 266667 to 266732 266681 to 266746 Chr1:176898010-176897945 6 120 0.994 800
FBXO15 FBX15 201456 ADS9475 5′-Upstream -296 to -227 -216 to -147
MMUT MCM 4594 ADS9479 5′-Upstream -3889 to -3833 -164 to -108 Chr6:49463355-49463299 5 100 0.988 300
N116P2 TTC25 n/a 83538 ADS9468 5′-Upstream -263 to -163 -195 to -95 Chr17:41930461-41930561 10 160 0.983 150
3-plex BBS4 n/a 585 ADS9487 Intron 1 301 to 377 342 to 418 Chr15:72686528-72686604 3 137 0.990 100
Ta = 55 CDH8 n/a 1006 ADS9488 Intron 1 -13197 to -13128 2236 to 2305 Chr16:62034600-62034531 7 113 0.858 100
N116P3 SIN3A WITKOS 25942 ADS9494 5′-Upstream -21838 to -21677 -509 to -348 Chr15:75452213-75452052 11 220 0.934 100
6-plex SRSF11 p54 9295 ADS9484 Intron 1 -15681 to -15558 233 to 356 Chr1:70205956-70206079 9 215 0.998 700
Ta = 56 MTR MS 4548 ADS9478 Intron 1 81 to 188 475 to 582 Chr1:236795784-236795891 12 151 0.979 100
CYB5B OMB5 80777 ADS9485 5′-Upstream -233 to -133 -144 to -44 Chr16:69424451-69424551 7 151 0.997 400
CUL3 PHA2E 8452 ADS9473 Intron 1 802 to 847 1141 to 1186 Chr2:224584208-224584163 4 108 0.846 300
EHHADH ECHD 1962 ADS9474 5′-Upstream -279 to -271 -203 to -195 Chr3:185254301-185254293 2 89 0.993 200
N116P4 CSNK2A1 CK2A1 1457 ADS9472 5′-Upstream, 5′-UTR -35334 to -35182 -62 to 91 Chr20:543885-543733 15 208 0.788 400
6-plex PRMT1 HRMT1L2 3276 ADS9482 5′-Upstream, 5′-UTR -178 to -61 -49 to 69 Chr19:49677103-49677220 11 178 0.815 400
Ta = 56  MAGT1 IAP 84061 ADS9471 5′-Upstream, 5′-UTR -144 to -21 -57 to 67 ChrX:77895650-77895527 11 174 0.879 1,000
JMJD1C TRIP8 221037 ADS9489 Intron 2 196442 to 196493 196661 to 196712 Chr10:63269221-63269170 2 117 0.956 400
MSANTD4 KIAA1826 84437 ADS9467 5′-Upstream -11405 to -11350 -95 to -40 Chr11:106022322-106022267 4 103 0.995 250
AHI1 JBTS3 54806 ADS9469 Intron 1 -5165 to -5128 344 to 381 Chr6:135497402-135497365 4 91 0.994 500
16 PCRs 55 genes 120 assays 719 CpGs 17,447 bp
NGS142 – Human EPHA2 (ephrin type-A receptor 2)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N142P1 EPHA2 ECK 1969 ASY2652 Exon 9, Intron 9 22031 to 22115 22168 to 22252 Chr1:16133902-16133818 8 153 0.845 900
8-plex EPHA2 ECK 1969 ASY2612 5′-Upstream -3559 to -3465 -3422 to -3328 Chr1:16159491-16159397 3 153 0.997 1,400
Ta = 59 EPHA2 ECK 1969 ASY2620 Intron 1 1534 to 1624 1671 to 1761 Chr1:16154399-16154309 4 141 0.863 700
EPHA2 ECK 1969 ASY2616 5′-Upstream -416 to -331 -279 to -194 Chr1:16156348-16156263 7 137 0.887 1,200
EPHA2 ECK 1969 ASY2636 Intron 3 11834 to 11887 11971 to 12024 Chr1:16144099-16144046 3 128 0.804 2,300
EPHA2 ECK 1969 ASY2641 Intron 3 16510 16647 Chr1:16139423 1 128 0.830 400
EPHA2 ECK 1969 ASY2647 Intron 6 20460 to 20475 20597 to 20612 Chr1:16135473-16135458 2 116 0.911 1,900
EPHA2 ECK 1969 ASY2645 Exon 5 17896 to 17948 18033 to 18085 Chr1:16138037-16137985 6 106 0.899 1,400
N142P2 EPHA2 ECK 1969 ASY2656 Exon 16 26344 to 26404 26481 to 26541 Chr1:16129589-16129529 8 155 0.864 400
6-plex EPHA2 ECK 1969 ASY2643 Intron 3 16854 to 16931 16991 to 17068 Chr1:16139079-16139002 3 149 0.877 300
Ta = 56 EPHA2 ECK 1969 ASY2637 Intron 3 11985 to 12021 12122 to 12158 Chr1:16143948-16143912 2 116 0.861 800
EPHA2 ECK 1969 ASY2608 5′-Upstream -6789 to -6759 -6652 to -6622 Chr1:16162721-16162691 3 100 0.994 200
EPHA2 ECK 1969 ASY2663 3′-Downstream 33044 to 33069 33181 to 33206 Chr1:16122889-16122864 3 99 0.966 1,200
EPHA2 ECK 1969 ASY2650 Intron 8 21569 to 21607 21706 to 21744 Chr1:16134364-16134326 4 92 0.898 400
N143P3 EPHA2 ECK 1969 ASY2639 Intron 3 12404 12541 Chr1:16143529 1 154 0.802 200
9-plex EPHA2 ECK 1969 ASY2624 Intron 1 4543 to 4587 4680 to 4724 Chr1:16151390-16151346 2 120 0.916 1,300
Ta = 56 EPHA2 ECK 1969 ASY2619 Intron 1 742 to 792 879 to 929 Chr1:16155191-16155141 4 110 0.795 700
EPHA2 ECK 1969 ASY2618 Intron 1 435 to 490 572 to 627 Chr1:16155498-16155443 5 106 0.873 300
EPHA2 ECK 1969 ASY2627 Exon 3 7287 to 7338 7424 to 7475 Chr1:16148646-16148595 5 102 0.842 1,400
EPHA2 ECK 1969 ASY2607 5′-Upstream -6887 to -6840 -6750 to -6703 Chr1:16162819-16162772 3 98 0.985 6,000
EPHA2 ECK 1969 ASY2657 Intron 16 27386 to 27411 27523 to 27548 Chr1:16128547-16128522 2 96 0.909 2,200
EPHA2 ECK 1969 ASY2651 Intron 8 21892 to 21904 22029 to 22041 Chr1:16134041-16134029 2 95 0.914 1,000
EPHA2 ECK 1969 ASY2633 Intron 3 10571 to 10596 10708 to 10733 Chr1:16145362-16145337 4 85 0.823 400
N142P4 EPHA2 ECK 1969 ASY2614 5′-Upstream -1291 to -1220 -1154 to -1083 Chr1:16157223-16157152 5 144 0.919 700
7-plex EPHA2 ECK 1969 ASY2646 Exon 5 18001 to 18050 18138 to 18187 Chr1:16137932-16137883 5 123 0.857 400
Ta = 53 EPHA2 ECK 1969 ASY2660 Intron 16 28315 to 28361 28452 to 28498 Chr1:16127618-16127572 3 117 0.882 400
EPHA2 ECK 1969 ASY2644 Intron 3 17101 to 17149 17238 to 17286 Chr1:16138832-16138784 3 117 0.903 900
EPHA2 ECK 1969 ASY2658 Intron 16 27594 to 27658 27731 to 27795 Chr1:16128339-16128275 4 116 0.830 500
EPHA2 ECK 1969 ASY2623 Intron 1 3536 to 3565 3673 to 3702 Chr1:16152397-16152368 2 116 0.905 400
EPHA2 ECK 1969 ASY2617 5′-Upstream -198 to -180 -61 to -43 Chr1:16156130-16156112 4 75 0.946 1,600
4 PCRs 1 gene 30 assays 111 CpGs 3,547 bp
NGS140 – Human Metabolism
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N140P1 PPARGC1A PGC1A 10891 ASY1845 Intron 2 14068 to 14292 14158 to 14382 Chr4:23875890-23875666 4 275 0.978 250
6-plex PPARGC1A PGC1A 10891 ASY1844 Intron 1 3797 to 3927 3887 to 4017 Chr4:23886161-23886031 3 205 0.968 2,000
Ta = 62 INS n/a 3630 ASY1823 5′-Upstream -1680 to -1624 -1447 to -1391 Chr11:2162651-2162595 5 197 0.226 500
PIK3R5 p101 23533 ASY1828 5′-Upstream -54295 to -54233 -77 to -15 Chr17:8965789-8965727 9 102 0.956 600
PPARGC1A PGC1A 10891 ASY1846 Intron 7 67656 67746 Chr4:23822302 1 100 0.990 7,500
INS n/a 3630 ASY3006 5′-UTR -112 to -100 122 to 134 Chr11:2161083-2161071 2 62 0.396 4,500
N140P2 PPARGC1A PGC1A 10891 ASY1847 Intron 12 92946 to 9305 93036 to 93145 Chr4:23797012-23796903 3 195 0.971 200
6-plex PPARA NR1C1 5465 ASY1654R 5′-Upstream -48806 to -48685 -2306 to -2185 Chr22:46149578-46149699 14 168 0.759 500
Ta = 59 INS n/a 3630 ADS3196 Intron 1 561 to 652 794 to 885 Chr11:2160411-2160320 7 154 0.312 150
PPARGC1A PGC1A 10891 ASY1840 5′-Upstream -961 to -903 -871 to -813 Chr4:23890918-23890860 3 149 0.994 1,800
PIK3R5 p101 23533 ASY1829 Intron 1 -4755 to -4719 49464 to 49500 Chr17:8916249-8916213 3 112 0.961 1,200
INS n/a 3630 ASY1822 5′-Upstream -1765 to -1716 -1532 to -1483 Chr11:2162736-2162687 3 105 0.211 700
N140P3 PPARGC1A PGC1A 10891 ADS2164 5′-UTR, Intron 1 -17 to 94 74 to 184 Chr4:23889974-23889864 6 174 0.992 800
5-plex PPARGC1A PGC1A 10891 ASY1841 Intron 1 604 to 699 694 to 789 Chr4:23889354-23889259 8 165 0.986 600
Ta = 56 INS n/a 3630 ADS1531 5′-Upstream -373 to -307 -140 to -74 Chr11:2161344-2161278 3 138 0.292 1,100
PIK3R5 p101 23533 ASY1824 Exon 10 22875 to 22951 77093 to 77169 Chr17:8888620-8888544 8 131 0.740 5,000
PPARGC1A PGC1A 10891 ASY1837 5′-Upstream -1310 -1220 Chr4:23891267 1 97 0.982 2,200
N140P4 PIK3R5 p101 23533 ASY1825 Exon 10 22719 to 22825 76937 to 77043 Chr17:8888776-8888670 7 183 0.868 500
5-plex PPARA NR1C1 5465 ASY1652 5′-Upstream -48959 to -48854 -2459 to -2354 Chr22:46149425-46149530 7 175 0.781 1,300
Ta = 56 PIK3R5 p101 23533 ASY1827 5′-Upstream -55565 to -55513 -1347 to -1295 Chr17:8967059-8967007 4 142 0.955 3,400
PPARGC1A PGC1A 10891 ASY1842 Intron 1 879 to 965 969 to 1055 Chr4:23889079-23888993 6 141 0.987 750
PPARGC1A PGC1A 10891 ASY1838 5′-Upstream -1042 -952 Chr4:23890999 1 106 0.995 1,100
N135P1 INSR CD220 3643 ADS1572 Intron 2 48226 to 48411 48749 to 48934 Chr19:7245666-7245481 5 256 0.985 3,700
4-plex WNT10B WNT12 7480 ASY1662 5′-Upstream -3171 to -3074 -1965 to -1868 Chr12:48973700-48973603 8 186 0.809 350
CAMK2B CAMK2 816 ASY1663 Intron 1 1103 to 1164 1276 to 1337 Chr7:44324319-44324258 6 175 0.889 100
N135P2 FZD8 FZ8 8325 ASY1658 5′-Upstream -2965 to -2911 -2116 to -2062 Chr10:35644394-35644340 3 168 0.874 2,800
5-plex WNT10B WNT12 7480 ASY1661 5′-Upstream -3272 to -3223 -2066 to -2017 Chr12:48973801-48973752 4 145 0.882 250
Ta = 56 CAMK2B CAMK2 816 ASY1656 Exon 1, Intron 1 25 to 95 198 to 268 Chr7:44325397-44325327 10 121 0.823 4,000
FZD8 FZ8 8325 ASY1659 5′-Upstream -2755 to -2735 -1906 to -1886 Chr10:35644184-35644164 3 116 0.869 1,600
CAMK2B CAMK2 816 ASY1653 5′-Upstream -551 to -499 -378 to -326 Chr7:44325972-44325920 8 107 0.909 3,800
N135P3 PPARA NR1C1 5465 ASY1655R 5′-Upstream -48653 to -48507 -2153 to -2007 Chr22:46149731-46149877 14 202 0.595 500
4-plex RXRA NR2B1 6256 ADS9116 Intron 1 36982 to 37088 37159 to 37265 Chr9:134363613-134363719 7 179 0.639 5,000
Ta = 59 RXRA NR2B1 6256 ADS9105 Intron 1 31362 to 31469 31539 to 31646 Chr9:134357993-134358100 8 160 0.658 4,000
RXRA NR2B1 6256 ADS9102 Intron 1 6834 to 6877 7011 to 7054 Chr9:134333465-134333508 6 103 0.719 1,600
N121P1 RARB NR1B2 5915 ADS179 5′-Upstream -529 to -503 -108 to -82 Chr3:25428203-25428229 3 133 0.986 1,500
5-plex SREBF1 SREBP1 6720 ASY1394 5′-Upstream -420 to -368 -250 to -198 Chr17:17837237-17837185 8 121 0.628 800
Ta = 59 SREBF2 SREBP2 6721 ASY1397 5′-Upstream -312 to -217 -146 to -111 Chr22:41832959-41832994 7 103 0.747 350
TP53 p53 7157 ADS9168 3′-UTR 7521 18408 Chr17:7669074 1 91 0.985 300
TP53 p53 7157 ADS9169 Exon 4 600 11487 Chr17:7675995 1 63 0.989 150
N121P2 TP53 p53 7157 ADS5682 5′-Upstream -11733 to -11597 -846 to -710 Chr17:7688327-7688191 12 193 0.838 2,700
4-plex PTEN MMAC1 5728 ADS3543 5′-Upstream -2487 to -2405 -1130 to -1048 Chr10:87861983-87862065 8 172 0.986 1,000
Ta = 59 SREBF1 SREBP1 6720 ASY1393 5′-Upstream -3224 to -3204 -3054 to -3034 Chr17:17840041-17840021 4 96 0.586 2,400
SREBF2 SREBP2 6721 ASY1398 Exon 1 50 to 87 216 to 253 Chr22:41833320-41833357 5 85 0.879 300
N121P4 SREBF1 SREBP1 6720 ASY1395 Intron 1 439 to 642 609 to 812 Chr17:17836379-17836176 26 250 0.709 100
4-plex PPARGC1A PGC1A 10891 ADS2154 5′-Upstream -380 to -214 -260 to -94 Chr4:23890337-23890171 4 238 0.980 150
Ta = 55 TP53 p53 7157 ADS5681 5′-Upstream -11927 to -11755 -1040 to -868 Chr17:7688521-7688349 23 217 0.958 250
TP53 p53 7157 ADS5683 5′-Upstream -11095 to -10966 -208 to -79 Chr17:7687689-7687560 7 210 0.954 150
N121P5 SREBF1 SREBP1 6720 ASY1396 Intron 1 12611 to 12796 12781 to 12966 Chr17:17824207-17824022 11 238 0.585 150
3-plex SREBF1 SREBP1 6720 ASY1392 5′-Upstream -3857 to -3766 -3687 to -3596 Chr17:17840674-17840583 8 198 0.758 500
Ta = 55 SREBF2 SREBP2 6721 ASY1399 Intron 1 575 to 709 741 to 875 Chr22:41833845-41833979 13 184 0.756 600
N121P8 PPARG NR1C3 5468 ADS2016B Intron 1 -91777 to -91690 562 to 649 Chr3:12287929-12288016 15 132 0.934 300
2-plex Ta = 55 TP53 p53 7157 ADS5685 5′-UTR -10848 to -10798 40 to 90 Chr17:7687442-7687392 7 107 0.961 250
N121P9 SIRT1 SIR2 23411 ASY1541 Intron 1 509 to 667 575 to 733 Chr10:67885230-67885388 19 209 0.988 2,100
5-plex SIRT1 SIR2 23411 ASY1543 Exon 4 6735 to 6775 6801 to 6841 Chr10:67891456-67891496 2 153 0.988 2,000
Ta = 53 SIRT1 SIR2 23411 ASY1540 5′-Upstream, 5′-UTR -87 to -32 -21 to 35 Chr10:67884635-67884690 11 118 0.984 5,400
SIRT1 SIR2 23411 ASY1539 5′-Upstream -394 to -352 -328 to -286 Chr10:67884328-67884370 4 108 0.970 3,000
SIRT1 SIR2 23411 ASY1542 Exon 3 4208 to 4233 4274 to 4299 Chr10:67888929-67888954 2 101 0.950 250
13 PCRs 16 genes 58 assays 396 CpGs 8,898 bp
NGS134 – Fibromyalgia-related genes (Human)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N134P1 TNFRSF13B TACI 23495 ASY1714 Exon 1, Intron 1 16 to 127 29 to 140 Chr17:16972060-16971949 7 226 0.991 150
4-plex TNFRSF13B TACI 23495 ASY1713 5′-Upstream -217 to -106 -204 to -93 Chr17:16972292-16972181 5 193 0.988 250
Ta = 62 OXT OT 5020 ASY1705 3′-Downstream 925 to 1003 961 to 1039 Chr20:3072580-3072658 9 140 0.825 250
GRM2 GLUR2 2912 ASY1639 5′-Upstream -2064 to -2044 -150 to -130 Chr3:51706920-51706940 3 70 0.648 100
N134P2 OXT OT 5020 ASY1700 5′-Upstream -319 to -187 -283 to -151 Chr20:3071337-3071469 6 189 0.858 500
6-plex TRPA1 FEPS 8989 ASY1646 Exon 1, Intorn 1 77 to 206 252 to 381 Chr8:72075333-72075204 12 186 0.917 150
Ta = 56 TRPA1 FEPS 8989 ASY1648 5′-Upstream -688 to -620 -513 to -445 Chr8:72076097-72076029 4 172 0.987 250
TRPA1 FEPS 8989 ASY1667 5′-Upstream -281 to -193 -106 to -18 Chr8:72075690-72075602 13 148 0.984 200
MCF2 DBL 4168 ASY1645 Intron 1 -40589 to -40500 15895 to 15984 ChrX:139692333-139692244 12 139 0.955 100
AVP ADH 551 ASY1706 5′-Upstream -7292 -7242 Chr20:3091966 1 76 0.764 300
N134P3 OXT OT 5020 ASY1702 5′-Upstream, 5′-UTR, Exon 1 -49 to 54 -13 to 90 Chr20:3071607-3071709 10 165 0.675 150
4-plex TNFRSF13B TACI 23495 ASY1715 Intron 1 223 to 260 236 to 273 Chr17:16971853-16971816 2 134 0.979 400
Ta = 56 GRM2 GLUR2 2912 ASY1638 5′-Upstream -2171 to -2104 -257 to -190 Chr3:51706813-51706880 9 121 0.552 1,000
IL25 IL17E 64806 ASY1644 5′-Upstream -835 to -815 -577 to -557 Chr14:23372284-23372304 2 119 0.733 100
N134P4 TRPA1 FEPS 8989 ADS2308 5′-Upstream -1037 to -928 -862 to -753 Chr8:72076446-72076337 5 225 0.981 100
7-plex DRD3 D3DR 1814 ASY1625 5′-Upstream -7033 to -6898 -327 to -192 Chr3:114179025-114178890 6 216 0.957 200
Ta = 53 DRD3 D3DR 1814 ASY1621 5′-Upstream -7771 to -7730 -1065 to -1024 Chr3:114179763-114179722 2 159 0.967 1,800
GRM2 GLUR2 2912 ASY1641 Intron 1 -1805 to -1714 110 to 201 Chr3:51707179-51707270 9 152 0.633 800
OXT OT 5020 ASY1701 5′-Upstream -155 to -78 -119 to -42 Chr20:3071501-3071578 7 137 0.736 1,800
NR3C1 GCR 2908 ADS255 Intron 1 -2363 to -2288 488 to 563 Chr5:143403202-143403127 13 132 0.873 500
AVP ADH 551 ASY1707 5′-Upstream -6064 -6014 Chr20:3090738 1 98 0.828 350
N134P5 TRPA1 FEPS 8989 ASY1651 5′-Upstream -379 to -320 -204 to -145 Chr8:72075788-72075729 7 147 0.953 100
4-plex TRPA1 FEPS 8989 ASY1647 5′-Upstream -836 -661 Chr8:72076245 1 104 0.993 150
Ta = 59 DRD3 D3DR 1814 ASY1622R 5′-Upstream -7391 -685 Chr3:114179383 1 96 0.976 350
C11orf40 NOV1 143501 ASY1709 5′-Upstream -1091 to -1071 -1091 to -1071 Chr11:4578911-4578891 2 73 0.995 250
N134P6 GCSAML C1orf150 148823 ASY1636 Intron 1 -45189 to -45003 11351 to 11537 Chr1:247518408-247518594 24 240 0.944 150
3-plex C11orf40 NOV1 143501 ASY1711 Intron 1 1962 to 2091 1962 to 2091 Chr11:4575859-4575730 6 211 0.979 250
Ta = 55 NR3C1 GCR 2908 ADS749 5′-Upstream -3260 to -3204 -410 to -354 Chr5:143404099-143404043 7 100 0.721 150
N134P7 MIR129-2 MIR-129b 406918 ADS8994 5′-Upstream n/a -250 to -87 Chr11:43581144-43581307 19 220 0.978 200
2-plex Ta = 59 SYT2 SytII 127833 ASY1642R Intron 1 -104127 to -104087 556 to 596 Chr1:202709899-202709859 5 102 0.861 500
7 PCRs 13 genes 30 assays 210 CpGs 4,490 bp

Mouse

NGS166 – Mouse Alb (Albumin)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm39) # CpGs Size (bp) RSQ Est. Cov.
N166P2 Alb Alb1 11657 ASY3602 Exon 13, Intron 13 13674 to 13817 13712 to 13855 Chr5:90622467-90622610 5 206 0.989 600
11-plex Alb Alb1 11657 ASY3594 Exon 8 7548 to 7612 7586 to 7650 Chr5:90616341-90616405 3 186 0.974 600
Ta = 56 Alb Alb1 11657 ASY3601 Intron 12 13392 to 13406 13430 to 13444 Chr5:90622185-90622199 2 174 0.973 400
Alb Alb1 11657 ASY3584 Intron 1 355 to 402 393 to 440 Chr5:90609148-90609195 3 170 0.985 200
Alb Alb1 11657 ASY3605 3′-Downstream 16549 to 16633 16587 to 16671 Chr5:90625342-90625426 3 155 0.988 600
Alb Alb1 11657 ASY3607 3′-Downstream 18951 to 19033 18989 to 19071 Chr5:90627744-90627826 2 118 0.995 200
Alb Alb1 11657 ASY3581 5′-Upstream  -2586 to -2524  -2548 to -2486 Chr5:90606208-90606270 2 138 0.999 200
Alb Alb1 11657 ASY3599 Intron 10 10256 to 10309 10294 to 10347 Chr5:90619049-90619102 2 120 0.986 400
Alb Alb1 11657 ASY3606 3′-Downstream 17817 to 17858 17855 to 17896 Chr5:90626610-90626651 2 151 0.995 2,900
Alb Alb1 11657 ASY3604 3′-Downstream 16074 to 16110 16112 to 16148 Chr5:90624867-90624903 2 92 0.999 700
Alb Alb1 11657 ASY3579 5′-Upstream -3621 -3583 Chr5:90605173 1 78 0.994 1,400
N166P3 Alb Alb1 11657 ASY3608 3′-Downstream 20260 20298 Chr5:90629053 1 139 0.981 100
8-plex Alb Alb1 11657 ASY3583 5′-Upstream  -184 to -110  -146 to -72 Chr5:90608610-90608684 2 136 0.975 4,400
Ta = 59 Alb Alb1 11657 ASY3600 Intron 12 13084 13122 Chr5:90621877 1 131 0.995 1,500
Alb Alb1 11657 ASY3598 Intron 9 9666 9704 Chr5:90618459 1 129 0.988 100
Alb Alb1 11657 ASY3582 5′-Upstream -2017 -1979 Chr5:90606777 1 90 0.992 800
Alb Alb1 11657 ASY3590 Intron 4 3802 3840 Chr5:90612595 1 81 0.997 300
Alb Alb1 11657 ASY3585 Intron 1 655 to 659 693 to 697 Chr5:90609448-90609452 2 80 0.998 2,100
Alb Alb1 11657 ASY3591 Exon 6 4979 5017 Chr5:90613772 1 80 0.997 6,100
N166P4 Alb Alb1 11657 ASY3592 Intron 6 5850 to 6033 5888 to 6071 Chr5:90614643-90614826 4 239 0.995 800
5-plex Alb Alb1 11657 ASY3596 Exon 9, Intron 9 8664 to 8783 8702 to 8821 Chr5:90617457-90617576 6 201 0.980 1,300
Ta = 59 Alb Alb1 11657 ASY3588 Exon 3 1808 to 1877 1846 to 1915 Chr5:90610601-90610670 4 181 0.992 3,800
Alb Alb1 11657 ASY3589 Exon 4 3092 to 3148 3130 to 3186 Chr5:90611885-90611941 3 162 0.995 1,100
Alb Alb1 11657 ASY3597 Intron 9 9282 9320 Chr5:90618075 1 91 0.985 700
Simplex Ta = 62 Alb Alb1 11657 ASY3611 Intron 10, Exon 11 10993 to 11122 11031 to 11160 Chr5:90619786-90619915 3 184 0.984 2,100
4 PCRs 1 gene 25 assays 58 CpGs 3,512 bp
NGS161 – Mouse Evl (Ena-vasodilator stimulated phosphoprotein)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm39) # CpGs Size (bp) RSQ Est. Cov.
N161P1 Evl n/a 14026 ASY3425 Intron 3 103273 to 103389 103758 to 103874 Chr12:108624740-108624856 5 203 0.973 100
14-plex Evl n/a 14026 ASY3426 Intron 3 114979 to 115058 115464 to 115543 Chr12:108636446-108636525 4 194 0.943 300
Ta = 62 Evl n/a 14026 ASY3419 Intron 1 62574 to 62702 63059 to 63187 Chr12:108584041-108584169 5 185 0.988 50
Evl n/a 14026 ASY3401 Intron 1 50588 to 50629 51073 to 51114 Chr12:108572055-108572096 3 142 0.976 200
Evl n/a 14026 ASY3395 5′-UTR  -193 to -111 293 to 375 Chr12:108521275-108521357 18 138 0.798 400
Evl n/a 14026 ASY3422 Intron 1 86990 87475 Chr12:108608457 1 135 0.006 200
Evl n/a 14026 ASY3410 Intron 1 81011 to 81061 81496 to 81546 Chr12:108602478-108602528 2 113 0.980 300
Evl n/a 14026 ASY3420 Intron 1 80646 to 80673 81131 to 81158 Chr12:108602113-108602140 2 105 0.953 1,600
Evl n/a 14026 ASY3412 Intron 3 102673 103158 Chr12:108624140 1 100 0.995 100
Evl n/a 14026 ASY3413 Exon 7 120941 to 120951 121426 to 121436 Chr12:108642408-108642418 2 98 0.939 200
Evl n/a 14026 ASY3403 Intron 1 50925 to 50938 51410 to 51423 Chr12:108572392-108572405 2 97 0.986 900
Evl n/a 14026 ASY3418 5′-Upstream  -2020 to -2014  -1535 tp -1529 Chr12:108519448-108519454 2 96 0.994 100
Evl n/a 14026 ASY3411 Intron 1 80415 80900 Chr12:108601882 1 89 0.845 100
Evl n/a 14026 ASY3417 5′-Upstream -2233 -1748 Chr12:108519235 1 77 0.951 50
N161P2 Evl n/a 14026 ASY3409 Intron 1 67315 to 67407 67800 to 67892 Chr12:108588782-108588874 3 184 0.986 100
10-plex Evl n/a 14026 ASY3396 Intron 1 12 to 100 497 to 585 Chr12:108521479-108521567 12 141 0.955 100
Ta = 56 Evl n/a 14026 ASY3427 Intron 11 130511 to 130575 130996 to 131060 Chr12:108651978-108652042 2 138 0.950 100
Evl n/a 14026 ASY3402 Intron 1 50786 to 50854 51271 to 51339 Chr12:108572253-108572321 3 137 0.973 100
Evl n/a 14026 ASY3399 Intron 1 37754 to 37796 38239 to 38281 Chr12:108559221-108559263 3 123 0.997 1,500
Evl n/a 14026 ASY3400 Intron 1 50464 to 50477 50949 to 50962 Chr12:108571931-108571944 2 120 0.987 100
Evl n/a 14026 ASY3414 Exon 14, 3′-UTR 132854 to 132912 133339 to 133397 Chr12:108654321-108654379 5 114 0.900 500
Evl n/a 14026 ASY3408 Intron 1 66701 to 66727 67186 to 67212 Chr12:108588168-108588194 2 111 0.992 400
Evl n/a 14026 ASY3423 Intron 3 101430 101915 Chr12:108622897 1 70 0.972 200
Evl n/a 14026 ASY3416 5′-Upstream -2429 -1944 Chr12:108519039 1 67 0.972 900
N161P3 Evl n/a 14026 ASY3391 5′-Upstream  -1091 to -995  -606 to -510 Chr12:108520377-108520473 4 225 0.988 100
5-plex Evl n/a 14026 ASY3405 Intron 1 51278 to 51416 51763 to 51901 Chr12:108572745-108572883 6 217 0.988 100
Ta = 57 Evl n/a 14026 ASY3404 Intron 1 51012 to 51098 51497 to 51583 Chr12:108572479-108572565 3 204 0.981 300
Evl n/a 14026 ASY3407 Intron 1 65832 to 65956 66317 to 66441 Chr12:108587299-108587423 4 200 0.989 100
Evl n/a 14026 ASY3398 Intron 1 313 to 364 798 to 849 Chr12:108521780-108521831 3 143 0.959 50
4 PCRs 1 gene 29 assays 103 CpGs 3,966 bp
NGS159 – Mouse Arc (activity regulated cytoskeletal-associated protein)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N159P1 Arc Arg3.1 11838 ASY3351 Exon 1 289 to 433 487 to 631 Chr15:74672084-74671940 11 229 0.977 100
6-plex Arc Arg3.1 11838 ASY3354 Exon 1 779 to 916 977 to 1114 Chr15:74671594-74671457 7 207 0.980 100
Ta = 56 Arc Arg3.1 11838 ASY3347 5′-Upstream -380 to -278 -182 to -80 Chr15:74672752-74672650 14 150 0.975 100
Arc Arg3.1 11838 ASY3353 Exon 1 614 to 685 812 to 883 Chr15:74671759-74671688 4 130 0.966 3,800
Arc Arg3.1 11838 ASY3350 Exon 1 24 to 66 222 to 264 Chr15:74672349-74672307 7 105 0.879 700
Arc Arg3.1 11838 ASY3345 5′-Upstream -586 to -562 -388 to -364 Chr15:74672958-74672934 2 99 0.974 4,200
N159P2 Arc Arg3.1 11838 ADS6522 Exon 1 103 to 256 301 to 454 Chr15:74672270-74672117 12 209 0.927 700
4-plex Arc Arg3.1 11838 ASY3346 5′-Upstream -483 to -419 -285 to -221 Chr15:74672855-74672791 5 146 0.995 300
Ta = 51 Arc Arg3.1 11838 ASY3355 5′-Upstream -6990 to -6910 -6792 to -6712 Chr15:74679362-74679282 4 130 0.972 5,100
Arc Arg3.1 11838 ASY3352 Exon 1 504 to 537 702 to 735 Chr15:74671869-74671836 3 100 0.937 2,100
2 PCRs 1 gene 10 assays 69 CpGs 1,505 bp
NGS156 – Mouse Jarid2 (jumonji, AT rich interactive domain 2)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N156P1 Jarid2 Jmj 16468 ASY3162 5′-Upstream -1157 to -1122 -556 to -521 Chr13:44730715-44730750 4 115 0.865 700
4-plex Jarid2 Jmj 16468 ASY3170 Intron 2 108192 to 108219 108793 to 108820 Chr13:44840063-44840090 2 96 0.992 500
Ta = 59 Jarid2 Jmj 16468 ASY3188 Exon 10 178525 to 178563 179126 to 179164 Chr13:44910396-44910434 3 111 0.972 300
Jarid2 Jmj 16468 ASY3190 3′-UTR 188691 to 188728 189292 to 189329 Chr13:44920562-44920599 3 124 0.920 1300
N156P2 Jarid2 Jmj 16468 ASY3160 5′-Upstream -2211 to -2124 -1610 to -1523 Chr13:44729661-44729748 9 162 0.875 50
4-plex Jarid2 Jmj 16468 ASY3168 Intron 1 39152 to 39183 39753 to 39784 Chr13:44771023-44771054 3 157 0.962 50
Ta = 59 Jarid2 Jmj 16468 ASY3185 Intron 8 174879 to 174908 175480 to 175509 Chr13:44906750-44906779 2 82 0.954 300
Jarid2 Jmj 16468 ASY3186 Intron 9 176115 to 176136 176716 to 176737 Chr13:44907986-44908007 2 93 0.980 400
N156P3 Jarid2 Jmj 16468 ASY3163 5′-Upstream -704 to -649 -103 to -48 Chr13:44731168-44731223 6 131 0.964 600
4-plex Jarid2 Jmj 16468 ASY3167 Intron 1 35922 to 35937 36523 to 36538 Chr13:44767793-44767808 2 94 0.990 2000
Ta = 59 Jarid2 Jmj 16468 ASY3179 Intron 4 148554 149155 Chr13:44880425 1 74 0.987 300
Jarid2 Jmj 16468 ASY3180 Intron 5 158766 159367 Chr13:44890637 1 119 0.993 600
N156P4 Jarid2 Jmj 16468 ASY3166 Intron 1 2149 to 2235 2750 to 2836 Chr13:44734020-44734106 9 156 0.961 100
4-plex Jarid2 Jmj 16468 ASY3169 Intron 1 48158 48759 Chr13:44780029 1 61 0.989 100
Ta = 59 Jarid2 Jmj 16468 ASY3178 Intron 3 125037 to 125120 125638 to 125721 Chr13:44856908-44856991 3 139 0.998 2200
Jarid2 Jmj 16468 ASY3184 Intron 7 172605 173206 Chr13:44904476 1 132 0.936 900
N156P5 Jarid2 Jmj 16468 ASY3164 Exon 1, Intron 1 45 to 89 646 to 690 Chr13:44731916-44731960 2 136 0.907 400
5-plex Jarid2 Jmj 16468 ASY3173 Intron 2 110157 to 110181 110758 to 110782 Chr13:44842028-44842052 2 134 0.996 50
Ta = 53 Jarid2 Jmj 16468 ASY3175 Intron 2 112064 to 112117 112665 to 112718 Chr13:44843935-44843988 3 184 0.996 50
Jarid2 Jmj 16468 ASY3176 Intron 3 119607 to 119651 120208 to 120252 Chr13:44851478-44851522 2 137 0.990 300
Jarid2 Jmj 16468 ASY3181 Intron 5 159670 to 159719 160271 to 160320 Chr13:44891541-44891590 2 103 0.987 300
Simplex Ta = 59 Jarid2 Jmj 16468 ASY3159 5′-Upstream -2453 to -2351 -1852 to -1750 Chr13:44729419-44729521 6 169 0.865 1900
6 PCRs 1 gene 22 assays 69 CpGs 2,709 bp
NGS154 – Mouse Pdgfb (platelet derived growth factor) & Mapk1 (mitogen-activated protein kinase 1)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N154P1 Pdgfb Sis 18591 ASY3114 5′-Upstream -5503 to -5387 -4693 to -4577 Chr15:80019670-80019554 6 215 0.913 600
6-plex Mapk1 Erk 26413 ASY3108 Intron 1 22623 to 22698 22868 to 22943 Chr16:17006249-17006324 2 173 0.957 100
Ta = 56 Pdgfb Sis 18591 ASY3118 Intron 4 13408 to 13472 14218 to 14282 Chr15:80000760-80000696 5 152 0.907 1700
Mapk1 Erk 26413 ASY3106 Intron 1 13028 to 13066 13273 to 13311 Chr16:16996654-16996692 2 114 0.996 800
Pdgfb Sis 18591 ASY3117 Intron 1 7578 to 7621 8388 to 8431 Chr15:80006590-80006547 3 92 0.962 200
Mapk1 Erk 26413 ASY3107 Intron 1 20085 to 20108 20330 to 20353 Chr16:17003711-17003734 2 84 0.995 6800
N154P2 Mapk1 Erk 26413 ASY3104 5′-Upstream -580 to -490 -335 to -245 Chr16:16983047-16983137 4 163 0.965 1700
6-plex Pdgfb Sis 18591 ASY3116 Intron 1 756 to 849 1566 to 1659 Chr15:80013412-80013319 9 146 0.860 900
Ta = 59 Pdgfb Sis 18591 ASY3115 5′-Upstream -1198 to -1144 -388 to -334 Chr15:80015365-80015311 2 144 0.944 4900
Mapk1 Erk 26413 ASY3110 Intron 1 28247 to 28291 28492 to 28536 Chr16:17011873-17011917 2 105 0.986 1200
Mapk1 Erk 26413 ASY3112 Intron 1 10785 11030 Chr16:16994411 1 96 0.997 9100
Mapk1 Erk 26413 ASY3105 Intron 1 530 to 560 775 to 805 Chr16:16984156-16984186 4 90 0.956 1500
Simplex Ta = 62 Mapk1 Erk 26413 ASY3113 5′-Upstream, 5′-UTR -398 to -208 -153 to 38 Chr16:16983229-16983419 22 272 0.783 100
3 PCRs 2 genes 13 assays 64 CpGs 1,846 bp
NGS150V1 – Mouse Elovl2 (elongation of very long chain fatty acids 2)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N150V1P1 Elovl2 Ssc2 54326 ASY2900 Intron 2 23911 to 24061 23959 to 24109 Chr13:41196447-41196297 4 201 0.917 100
9-plex Elovl2 Ssc2 54326 ASY2906 Intron 1 7395 to 7447 7443 to 7495 Chr13:41212963-41212911 3 151 0.935 1,800
Ta = 62 Elovl2 Ssc2 54326 ASY2861 5′-Upstream -467 to -412 -419 to -364 Chr13:41220824-41220769 6 146 0.973 400
Elovl2 Ssc2 54326 ASY2894 Exon 2, Intron 2 23220 to 23265 23268 to 23313 Chr13:41197138-41197093 2 142 0.972 1,700
Elovl2 Ssc2 54326 ASY2896 Exon 4, Intron 4 28443 to 28514 28491 to 28562 Chr13:41191915-41191844 3 138 0.955 200
Elovl2 Ssc2 54326 ASY2905 Intron 1 16952 to 17020 17000 to 17068 Chr13:41203406-41203338 3 130 0.980 1,400
Elovl2 Ssc2 54326 ASY2907 Intron 1 7664 to 7695 7712 to 7743 Chr13:41212694-41212663 2 106 0.928 1,500
Elovl2 Ssc2 54326 ASY2898 Exon 8, 3′-UTR 35045 to 35075 35093 to 35123 Chr13:41185313-41185283 2 81 0.870 2,700
Elovl2 Ssc2 54326 ASY2902 3′-Downstream 39620 39668 Chr13:41180738 1 80 0.823 3,300
N150V1P2 Elovl2 Ssc2 54326 ASY2890 Intron 1 294 to 424 342 to 472 Chr13:41220064-41219934 9 197 0.984 1,200
7-plex Elovl2 Ssc2 54326 ASY2888 5′-Upstream -365 to -255 -317 to -207 Chr13:41220722-41220612 18 164 0.981 600
Ta = 59 Elovl2 Ssc2 54326 ASY2885 5′-Upstream -678 to -614 -630 to -566 Chr13:41221035-41220971 3 134 0.956 1,600
Elovl2 Ssc2 54326 ASY2892 Intron 1 1941 to 1992 1989 to 2040 Chr13:41218417-41218366 3 130 0.958 3,200
Elovl2 Ssc2 54326 ASY2887 5′-Upstream -879 to -853 -831 to -805 Chr13:41221236-41221210 3 101 0.903 400
Elovl2 Ssc2 54326 ASY2891 Intron 1 838 to 852 886 to 900 Chr13:41219520-41219506 2 97 0.934 9,000
Elovl2 Ssc2 54326 ASY2886 5′-Upstream -777 -729 Chr13:41221134 1 78 0.835 500
2 PCRs 1 gene 16 assays 65 CpGs 2,076 bp
NGS146 – Mouse CNS (central nervous system)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N146P1 Oxtr Otr 18430 ASY2807 Exon 1 694 840 Chr6:112489104-112489026 12 207 0.550 100
7-plex Grin2b Nr2b 14812 ADS662 Intron 1 -128023 to -127927 802 to 898 Chr6:136172324-136172228 12 154 0.857 500
Ta = 62 Fkbp5 Dit 14229 ADS3545 Intron 4 -257 to -212 76236 to 76281 Chr17:28441289-28441244 3 136 0.717 250
Crh Crf 12918 ADS444 Intron 1 -211 to -150 710 to 771 Chr3:19694687-19694626 7 126 0.681 200
Foxp2 Spch1 114142 ADS1916 Intron 3 -15399 to -15363 280214 to 280250 Chr6:15181562-15181598 4 124 0.605 400
Sox1 Sox 20664 ASY2814 5′-Upstream -1481 to -1423 -415 to -357 Chr8:12394880-12394938 5 120 0.621 300
Atrx Xh2 22589 ADS1540B 5′-Upstream -547 to -522 -263 to -238 ChrX:105929666-105929641 2 118 0.762 300
N146P2 Oxtr Otr 18430 ASY2805 5′-Upstream -989 to -858 -843 to -712 Chr6:112490786-112490655 9 193 0.478 100
7-plex Oxtr Otr 18430 ASY2806 5′-UTR, Exon 1 -86 to 45 61 to 191 Chr6:112489883-112489753 15 148 0.615 200
Ta = 59 Oxtr Otr 18430 ASY2804 5′-Upstream -1360 to -1296 -1214 to -1150 Chr6:112491157-112491093 8 136 0.532 450
Nr3c1 Gcr 14815 ADS5418 Intron 1 -3006 to -2946 437 to 497 Chr18:39490238-39490178 6 118 0.680 300
Ezh2 Enx1 14056 ASY2822 5′-Upstream, 5′-UTR -17389 to -17343 -19 to 28 Chr6:47595049-47595003 9 104 0.863 250
Mecp2 Mbd5 17257 ADS5416A Intron 1 -29036 26394 ChrX:74108970 1 101 0.868 300
Bdnf Buln2 12064 ADS135A Intron 1 16895 17532 Chr2:109692231 1 73 0.601 450
N146P3 Bdnf Buln2 12064 ADS135B Intron 1 16952 to 17013 17589 to 17650 Chr2:109692288-109692349 4 159 0.577 200
7-plex Sox1 Sox 20664 ASY2813 5′-Upstream -2265 to -2183 -1199 to -1177 Chr8:12394096-12394178 5 132 0.658 150
Ta = 56 Gfra1 Gdnfr 14585 ADS932 5′-UTR -949 to -895 102 to 156 Chr19:58455297-58455243 5 118 0.738 750
Pde4d Dpde3 238871 ADS687 Intron 3 825793 to 825843 1031782 to 1031832 Chr13:109685958-109686008 5 109 0.850 350
Sox1 Sox 20664 ASY2826 Exon 1 148 to 199 1214 to 1265 Chr8:12396508-12396559 7 103 0.622 100
Ezh2 Enx1 14056 ASY2824 Intron 1 -16641 to -16604 730 to 767 Chr6:47594301-47594264 6 86 0.663 200
Nr3c2 Mcr 110784 ADS5423 Intron 3 189513 198013 Chr8:77097784 1 86 0.835 300
N146P4 Fkbp5 Dit 14229 ADS5316 Intron 4 -867 to -739 75626 to 75754 Chr17:28441899-28441771 3 193 0.712 200
7-plex Bdnf Buln2 12064 ADS431B Intron 1 17065 to 17164 17702 to 17801 Chr2:109692401-109692500 8 178 0.642 500
Ta = 53 Ezh2 Enx1 14056 ASY2825 Intron 1 -16182 to -16107 1189 to 1264 Chr6:47593842-47593767 5 145 0.747 1,700
Nr3c1 Gcr 14815 ADS5419A Intron 1 -2920 to -2832 523 to 611 Chr18:39490152-39490064 20 130 0.609 250
Bdnf Buln2 12064 ADS5403B Intron 1 16751 17388 Chr2:109692087 1 111 0.639 750
Crh Crf 12918 ADS310A 5′-Upstream -1091 to -1065 -171 to -145 Chr3:19695567-19695541 4 105 0.625 100
Nr3c1 Gcr 14815 ADS5421 Intron 2 45874 49316 Chr18:39441359 1 105 0.760 200
4 PCRs 14 genes 28 assays 169 CpGs 3,618 bp
NGS137V2 – Mouse Imprinted Genes
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N137V2P6 Kcnq1ot1 Lit1 63830 ADS913 Exon 1 n/a 1208 to 1360 Chr7:143295342-143295190 9 209 0.963 200
10-plex Mest Peg1 17294 ASY1686 5′-Upstream -701 to -559 -441 to -299 Chr6:30737609-30737751 7 207 0.939 400
Ta = 58 Peg3 Pw1 18616 ADS190 5′-UTR, Intron 1 -11901 to -11765 80 to 216 Chr7:6730342-6730206 11 192 0.991 800
H19 Asm 14955 ADS445 5′-Upstream n/a -2643 to -2574 Chr7:142580786-142580717 6 181 0.680 300
IG-DMR n/a n/a ADS1452S Intergenic n/a n/a Chr12:109528375-109528471 18 175 0.890 100
Sgce e-SG 20392 ASY1684 Exon 1, Intron 1 47 to 126 135 to 214 Chr6:4747042-4746963 7 152 0.966 300
Zdbf2 Slx9 73884 ASY1814 5′-Upstream -27100 to -27040 -9627 to -9567 Chr1:63263638-63263698 5 149 0.987 200
Peg3 Pw1 18616 ASY1691 5′-Upstream -12159 to -12065 -179 to -85 Chr7:6730600-6730506 6 145 0.985 300
Rasgrf1 Gnrp 19417 ASY1722 5′-Upstream -34119 to -34085 -34030 to -33996 Chr9:89875879-89875913 3 113 0.939 1,000
Snrpn Peg4 20646 ASY1694 Intron 2 -16297 to -16249 211 to 259 Chr7:60004901-60004853 6 105 0.991 400
N137V2P7 Peg13 n/a 353342 ASY1806 Exon 1 n/a 697 to 842 Chr15:72809627-72809482 12 223 0.886 100
9-plex Mir127 n/a 387146 ADS1374 Exon 1, 3’-Downstream n/a 43 to 169 Chr12:109592889-109593015 8 196 0.926 100
Ta = 54 Rasgrf1 Gnrp 19417 ASY1721 5′-Upstream -35491 to -35386 -35402 to -35297 Chr9:89874507-89874612 4 171 0.920 200
Airn Air 104103 ASY1809R Intron 1 n/a 1362 to 1189 Chr17:12742271-12742174 10 164 0.972 900
Mest Peg1 17294 ADS915B 5′-Upstream -1294 to -1249 -1034 to -989 Chr6:30737016-30737061 3 123 0.885 400
Peg13 n/a 353342 ASY1807 Exon 1 n/a 997 to 1052 Chr15:72809327-72809272 4 115 0.843 100
Nespas Gnas 14683 ASY1679 Intron 1 n/a 377 to 410 Chr2:174295026-174295059 5 110 0.966 100
Sgce e-SG 20392 ASY1682 5′-Upstream -2070 to -2050 -1982 to -1962 Chr6:4749158-4749138 7 86 0.832 500
Grb10 Meg1 14783 ASY1803 Intron 1 -55144 to -55122 11642 to 11664 Chr11:12025760-12025738 2 83 0.972 900
N137V2P8 Airn Air 104103 ASY1810R Intron 1 n/a 931 to 834 Chr17:12742702-12742529 8 237 0.987 200
9-plex IG-DMR n/a n/a ADS8998B Intergenic n/a n/a Chr12:109526596-109526736 9 236 0.875 200
Ta = 51 Sgce e-SG 20392 ASY1683 5′-Upstream -780 to -648 -692 to -560 Chr6:4747868-4747736 15 183 0.834 100
Gnasxl Gnas 14683 ASY1680 5′-Upstream -1426 to -1318 -1423 to -1315 Chr2:174296436-174296544 8 158 0.883 100
Zdbf2 Slx9 73884 ASY1716 5′-Upstream -26927 to -26879 -9454 to -9406 Chr1:63263811-63263859 4 146 0.960 600
Plagl1 Zac1 22634 ADS191 Intron 1 -33977 to -33890 253 to 340 Chr10:13091040-13091127 13 137 0.993 600
Mest Peg1 17294 ASY1687 Exon 1, Intron 1 7 to 86 267 to 346 Chr6:30738316-30738395 10 133 0.881 800
Impact Rwdd5 16210 ASY1812 Intron 1 2120 to 2186 2534 to 2600 Chr18:12974456-12974522 5 118 0.953 200
Igf2r Cd222 16004 ADS2710re 5′-Upstream -432 to -400 -301 to -269 Chr17:12769965-12769933 4 83 0.912 400
N137V2P9 Kcnq1ot1 Lit1 63830 ADS912 Exon 1 n/a 1041 to 1208 Chr7:143295509-143295342 12 264 0.953 200
9-plex Dlk1 Peg9 13386 ADS6547 Exon 5 6050 to 6206 6255 to 6411 Chr12:109459709-109459865 16 239 0.917 100
Ta = 56 Nesp Gnas 14683 ADS3759 5′-Upstream -639 to -497 -285 to -143 Chr2:174284035-174284177 7 221 0.936 100
Gnas n/a 14683 ADS3756 5′-Upstream -1906 to -1752 -1616 to -1462 Chr2:174328390-174328544 17 216 0.849 100
H19 Asm 14955 ADS447 5′-Upstream n/a -2446 to -2358 Chr7:142580589-142580501 6 212 0.650 1,000
Igf2 Peg2 16002 ADS2052 5′-Upstream -13921 to -13824 -12235 to -12138 Chr7:142669731-142669634 9 203 0.969 400
IG-DMR n/a n/a ADS1373 Intergenic n/a n/a Chr12:109530388-109530457 7 196 0.930 200
H19 Asm 14955 ADS1702 5′-Upstream n/a -3737 to -3618 Chr7:142581880-142581761 9 183 0.530 100
Meg3 Gtl2 17263 ADS1341 Intron 1 n/a 713 to 807 Chr12:109541718-109541812 7 163 0.371 700
4 PCRs 20 genes 37 assays 298 CpGs* 6,227 bp
NGS129 – Skeletal Muscle-related genes (Mouse)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N129P1 Ubr5 Edd1 70790 ASY2368 Intron 1 26383 to 26419 26383 to 26419 Chr15:38052043-38052007 3 146 0.990 500
6-plex Agtr1a At1 11607 ASY2301 Intron 1 -38656 to -38618 5858 to 5896 Chr13:30342298-30342336 2 132 0.981 200
Ta = 62 Stag1 Sa1 20842 ASY2339 5′-Upstream -62514 to -62473 -736 to -695 Chr9:100642712-100642753 4 113 0.989 100
Bicc1 Bicc 83675 ASY2312 Intron 1 3887 to 3940 3971 to 4024 Chr10:71155730-71155677 5 109 0.952 600
Agtr1a At1 11607 ASY2295 5′-Upstream -44567 to -44514 -54 to -1 Chr13:30336387-30336440 4 106 0.964 800
Ubr5 Edd1 70790 ASY2365 Intron 1 12327 to 12360 12327 to 12360 Chr15:38066099-38066066 3 90 0.988 300
N129P2 Traf1 Ebi6 22029 ASY2355 Intron 3 1649 to 1740 5154 to 5245 Chr2:34956432-34956341 5 186 0.980 100
8-plex Ubr5 Edd1 70790 ASY2364 Intron 1 11719 to 11832 11719 to 11832 Chr15:38066707-38066594 4 174 0.992 300
Ta = 59 Odf2 Odf84 18286 ASY2331 Intron 2 -1461 to -1391 1692 to 1762 Chr2:29891586-29891656 3 164 0.927 400
Stag1 Sa1 20842 ASY2337 5′-Upstream -62858 to -62796 -1080 to -1018 Chr9:100642368-100642430 3 152 0.985 100
Odf2 Odf84 18286 ASY2329 5′-UTR, Intron 2 -2115 to -2025 1038 to 1128 Chr2:29890932-29891022 4 146 0.889 100
Ubr5 Edd1 70790 ASY2358 5′-Upstream -1129 to -1055 -1129 to -1055 Chr15:38079554-38079480 7 139 0.991 100
Odf2 Odf84 18286 ASY2324 5′-Upstream -3897 to -3835 -745 to -683 Chr2:29889150-29889212 5 135 0.921 400
Traf1 Ebi6 22029 ASY2348 5′-Upstream -5227 to -5172 -1722 to -1667 Chr2:34963307-34963252 2 117 0.925 100
N129P3 Stag1 Sa1 20842 ASY2343 Intron 1 -60643 to -60493 1136 to 1286 Chr9:100644583-100644733 8 201 0.986 100
11-plex Ubr5 Edd1 70790 ASY2363 Intron 1 9991 to 10125 9991 to 10125 Chr15:38068435-38068301 6 189 0.964 100
Ta = 59 Traf1 Ebi6 22029 ASY2344 5′-Upstream -7824 to -7713 -4319 to -4208 Chr2:34965904-34965793 5 168 0.897 400
Agtr1a At1 11607 ASY2300 Intron 1 -38268 to -38179 6246 to 6335 Chr13:30342686-30342775 7 164 0.918 100
Grik2 Glur6 14806 ASY2315 5′-Upstream -7006 to -6913 -1499 to -1406 Chr10:49790265-49790172 3 153 0.971 100
Ubr5 Edd1 70790 ASY2366 Intron 1 15221 to 15305 15221 to 15305 Chr15:38063205-38063121 4 148 0.986 500
Agtr1a At1 11607 ASY2297 Intron 1 -44270 to -44194 244 to 320 Chr13:30336684-30336760 6 147 0.976 150
Bicc1 Bicc 83675 ASY2307 5′-Upstream -598 to -539 -514 to -455 Chr10:71160214-71160155 3 144 0.955 1,200
Bicc1 Bicc 83675 ASY2311 Intron 1 3446 to 3514 3530 to 3598 Chr10:71156171-71156103 3 142 0.943 150
Grik2 Glur6 14806 ASY2323 Intron 2 4555 to 4586 10062 to 10093 Chr10:49778705-49778674 3 136 0.954 100
Traf1 Ebi6 22029 ASY2349 5′-Upstream -5048 to -4978 -1543 to -1473 Chr2:34963128-34963058 3 129 0.916 400
N129P4 Odf2 Odf84 18286 ASY2335 Exon 5 410 to 500 3562 to 3652 Chr2:29893456-29893546 7 176 0.928 250
8-plex Agtr1a At1 11607 ASY2304 Intron 2 -35235 to -35141 9279 to 9373 Chr13:30345719-30345813 6 154 0.947 200
Ta = 56 Bicc1 Bicc 83675 ASY2310 Intron 1 2787 to 2856 2871 to 2940 Chr10:71156830-71156761 3 154 0.953 1,100
Grik2 Glur6 14806 ASY2316 5′-Upstream -5738 to -5653 -231 to -146 Chr10:49788997-49788912 5 151 0.991 600
Agtr1a At1 11607 ASY2298 Intron 1 -42388 to -42304 2126 to 2210 Chr13:30338566-30338650 4 131 0.918 350
Ubr5 Edd1 70790 ASY2362 Intron 1 1222 to 1266 1222 to 1266 Chr15:38077204-38077160 4 116 0.951 150
Odf2 Odf84 18286 ASY2330 Intron 2 -1633 to -1594 1520 to 1559 Chr2:29891414-29891453 5 106 0.856 200
Odf2 Odf84 18286 ASY2328 Intron 1 -2695 to -2660 458 to 493 Chr2:29890352-29890387 5 105 0.879 100
N129P5 Agtr1a At1 11607 ASY2302 Intron 1 -36681 to -36609 7833 to 7905 Chr13:30344273-30344345 5 200 0.986 250
7-plex Grik2 Glur6 14806 ASY2313 5′-Upstream -7517 to -7429 -2010 to -1922 Chr10:49790776-49790688 3 190 0.961 100
Ta = 59 Stag1 Sa1 20842 ASY2341 5′-UTR -61565 to -61445 214 to 334 Chr9:100643661-100643781 15 168 0.961 100
Grik2 Glur6 14806 ASY2322 Intron 2 4191 to 4298 9698 to 9805 Chr10:49779069-49778962 4 165 0.965 500
Odf2 Odf84 18286 ASY2334 Intron 3 -326 to -223 2827 to 2930 Chr2:29892721-29892824 4 157 0.920 600
Traf1 Ebi6 22029 ASY2354 Intron 3 860 to 927 4365 to 4432 Chr2:34957221-34957154 3 123 0.965 400
Ubr5 Edd1 70790 ASY2361 Intron 1 1104 to 1170 1104 to 1170 Chr15:38077322-38077256 6 119 0.939 150
N129P6 Ubr5 Edd1 70790 ASY2367 Intron 1 21742 to 21871 21742 to 21871 Chr15:38056684-38056555 5 208 0.993 250
4-plex Grik2 Glur6 14806 ASY2317 5′-Upstream, 5′-UTR -5608 to -5466 -101 to 42 Chr10:49788867-49788725 20 194 0.950 100
Ta = 59 Traf1 Ebi6 22029 ASY2352 Intron 2 -359 to -322 3147 to 3184 Chr2:34958439-34958402 3 106 0.976 300
Ubr5 Edd1 70790 ASY2357 5′-Upstream -1180 to -1168 -1180 to -1168 Chr15:38079605-38079593 3 88 0.820 400
N129P7 Timp3 K222 21859 ASY2421 5′-Upstream -4655 to -4545 -4173 to -4063 Chr10:86296199-86296309 8 181 0.966 4,800
9-plex Cttn Ems1 13043 ASY2441 Intron 2 -1495 to -1458 5517 to 5554 Chr7:144465493-144465456 2 140 0.941 250
Ta = 59 Msn C78546 17698 ASY1546 5′-Upstream -597 to -537 -407 to -347 ChrX:96095635-96095695 2 135 0.991 200
Pax3 Hup2 18505 ASY2449 5′-Upstream -863 to -799 -774 to -710 Chr1:78197612-78197548 5 131 0.963 100
Pax3 Hup2 18505 ASY2447 5′-Upstream -1429 to -1381 -1340 to -1292 Chr1:78198178-78198130 4 123 0.926 300
Wnt9a Wnt14 216795 ASY2430 5′-Upstream -1625 to -1569 -1582 to -1526 Chr11:59305348-59305404 4 122 0.804 350
Pax3 Hup2 18505 ASY2446 5′-Upstream -2424 to -2368 -2335 to -2279 Chr1:78199173-78199117 3 115 0.928 1,600
Pax3 Hup2 18505 ASY2452 Intron 1 727 to 772 816 to 861 Chr1:78196023-78195978 6 106 0.929 4,000
Pax3 Hup2 18505 ASY2445 5′-Upstream -2581 to -2529 -2492 to -2440 Chr1:78199330-78199278 4 105 0.865 900
N129P8 Timp3 K222 21859 ASY2427 Exon 1, Intron 1 60 to 154 542 to 636 Chr10:86300913-86301007 7 163 0.949 1,500
8-plex Cttn Ems1 13043 ASY2439 Intron 1 -6457 to -6372 555 to 640 Chr7:144470455-144470370 9 163 0.985 1,000
Ta = 62 Pax3 Hup2 18505 ASY2450 Intron 1 105 to 193 194 to 282 Chr1:78196645-78196557 7 153 0.950 2,000
Msn C78546 17698 ASY1547 5′-Upstream -446 to -432 -256 to -242 ChrX:96095786-96095800 2 116 0.990 10,000
Msn C78546 17698 ASY1558 Intron 1 938 to 973 1128 to 1163 ChrX:96097169-96097204 2 115 0.991 400
Gsk3b Gsk 56637 ASY2032 Exon 1 5 to 32 1530 to 1557 Chr16:38090530-38090557 4 107 0.919 9,500
Msn C78546 17698 ASY1557 Intron 1 828 to 863 1018 to 1053 ChrX:96097059-96097094 2 105 0.993 1,200
Cttn Ems1 13043 ASY2436 5′-UTR -6924 to -6884 88 to 128 Chr7:144470922-144470882 5 100 0.934 2,000
N129P9 Wnt9a Wnt14 216795 ASY2435 Intron 1 1229 to 1330 1272 to 1373 Chr11:59308201-59308302 6 156 0.849 7,500
2-plex Ta = 59 Pax3 Hup2 18505 ASY2444 5′-Upstream -2795 to -2700 -2706 to -2611 Chr1:78199544-78199449 4 141 0.948 1,500
N129P10 Gsk3b Gsk 56637 ASY2031 5′-Upstream -2362 to -2217 -837 to -692 Chr16:38088164-38088309 10 240 0.936 500
7-plex Cttn Ems1 13043 ASY2440 Intron 1 -4948 to -4867 2064 to 2145 Chr7:144468946-144468865 4 179 0.983 200
Ta = 57 Cttn Ems1 13043 ASY2438 Intron 1 -6616 to -6504 396 to 508 Chr7:144470614-144470502 7 178 0.967 2,000
Pax3 Hup2 18505 ASY2451 Intron 1 238 to 357 327 to 446 Chr1:78196512-78196393 7 163 0.873 2,400
Msn C78546 17698 ASY1556 Intron 1 169 to 246 359 to 436 ChrX:96096400-96096477 3 152 0.990 3,800
Gsk3b Gsk 56637 ASY2033 Intron 1 347 to 417 1872 to 1942 Chr16:38090872-38090942 6 146 0.872 100
Wnt9a Wnt14 216795 ASY2433 5′-Upstream -433 to -386 -390 to -343 Chr11:59306540-59306587 5 117 0.850 450
N129P11 Msn C78546 17698 ASY1560 Intron 2 48785 to 48939 48975 to 49129 ChrX:96145016-96145170 3 221 0.991 400
7-plex Cttn Ems1 13043 ASY2437 5′-UTR -6824 to -6718 188 to 294 Chr7:144470822-144470716 17 188 0.934 100
Ta = 59 Wnt9a Wnt14 216795 ASY2434 Intron 1 946 to 1076 989 to 1119 Chr11:59307918-59308048 12 184 0.746 150
Msn C78546 17698 ASY1559 Intron 1 1113 to 1207 1303 to 1397 ChrX:96097344-96097438 2 170 0.995 300
Pax3 Hup2 18505 ASY2442 5′-Upstream -3962 to -3856 -3873 to -3767 Chr1:78200711-78200605 8 170 0.833 10,000
Wnt9a Wnt14 216795 ASY2431 5′-Upstream -1143 to -1032 -1100 to -989 Chr11:59305830-59305941 5 163 0.838 10,000
Pax3 Hup2 18505 ASY2453 Exon 2 1380 to 1424 1469 to 1513 Chr1:78195370-78195326 6 115 0.971 500
11 PCRs 13 genes 77 assays 396 CpGs 11,304 bp
NGS096/095 – Mouse Rxra & Vdr (retinoid X receptor alpha & vitamin D receptor)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N95P2 Rxra Nr2b1 20181 ADS9215 Intron 1 47789 to 47915 48021 to 48147 Chr2:27724460-27724586 5 192 0.841 50
6-plex Rxra Nr2b1 20181 ADS9219 Intron 2 62996 to 63097 63228 to 63329 Chr2:27739667-27739768 7 162 0.777 50
Ta = 62 Rxra Nr2b1 20181 ADS9159 Intron 1 6778 to 6837 7010 to 7069 Chr2:27683449-27683508 3 126 0.802 600
Rxra Nr2b1 20181 ADS9099 Intron 1 2452 to 2461 2684 to 2693 Chr2:27679123-27679132 2 110 0.743 350
Rxra Nr2b1 20181 ADS9229 Intron 1 13398 to 13425 13630 to 13657 Chr2:27690069-27690096 2 110 0.881 300
Rxra Nr2b1 20181 ADS9227 3′-Downstream 86629 to 86648 86861 to 86880 Chr2:27763300-27763319 3 109 0.852 50
N95P3 Rxra Nr2b1 20181 ADS9214 Intron 1 21597 to 21756 21829 to 21988 Chr2:27698268-27698427 6 212 0.974 50
6-plex Rxra Nr2b1 20181 ADS9040 Intron 1 1767 to 1832 1999 to 2064 Chr2:27678438-27678503 2 162 0.578 50
Ta = 62 Rxra Nr2b1 20181 ADS9228 Intron 1 11366 to 11437 11598 to 11669 Chr2:27688037-27688108 3 142 0.874 150
Rxra Nr2b1 20181 ADS9212 Intron 1 17560 to 17629 17792 to 17861 Chr2:27694231-27694300 5 138 0.840 400
Rxra Nr2b1 20181 ADS9216 Intron 1 51164 to 51194 51396 to 51426 Chr2:27727835-27727865 5 95 0.724 1,000
Rxra Nr2b1 20181 ADS9224 Intron 5 71722 to 71747 71954 to 71979 Chr2:27748393-27748418 3 82 0.824 150
N96P2 Vdr Nr1i1 22337 ADS9232 Intron 1 -15032 to -14972 8340 to 8400 Chr15:97899971-97899911 3 127 0.922 1,000
6-plex Vdr Nr1i1 22337 ADS9239 Intron 3 7092 to 7145 30463 to 30516 Chr15:97877848-97877795 3 125 0.965 250
Ta = 59 Vdr Nr1i1 22337 ADS9236 Intron 1 -4177 to -4117 19195 to 19255 Chr15:97889116-97889056 3 122 0.996 200
Vdr Nr1i1 22337 ADS9240 Intron 4 13667 to 13678 37038 to 37049 Chr15:97871273-97871262 2 122 0.950 500
Vdr Nr1i1 22337 ADS9243 Intron 7 23530 to 23573 46901 to 46944 Chr15:97861410-97861367 3 120 0.848 1,200
Vdr Nr1i1 22337 ADS9237 Intron 3 4082 to 4140 27453 to 27511 Chr15:97880858-97880800 4 112 0.927 1,000
N96P3 Vdr Nr1i1 22337 ADS9241 Intron 7 19603 to 19672 42974 to 43043 Chr15:97865337-97865268 4 175 0.834 70
6-plex Vdr Nr1i1 22337 ADS9231 Intron 1 -19446 3926 Chr15:97904385 6 164 0.855 150
Ta = 59 Vdr Nr1i1 22337 ADS9244 3′-Downstream 35299 to 35403 58670 to 58774 Chr15:97849641-97849537 10 161 0.689 50
Vdr Nr1i1 22337 ADS9234 Intron 1 -7711 to -7644 15661 to 15728 Chr15:97892650-97892583 4 146 0.922 300
Vdr Nr1i1 22337 ADS9238 Intron 3 6134 to 6200 29505 to 29571 Chr15:97878806-97878740 3 144 0.938 700
Vdr Nr1i1 22337 ADS9233 Intron 1 -11184 to -11128 12188 to 12244 Chr15:97896123-97896067 5 134 0.977 400
N96P4 Vdr Nr1i1 22337 ADS9235 Intron 3 1526 to 1565 24897 to 24936 Chr15:97883414-97883375 3 109 0.887 600
3-plex Vdr Nr1i1 22337 ADS9242 Intron 7 20960 to 20997 44331 to 44368 Chr15:97863980-97863943 4 100 0.865 300
Ta = 53 Vdr Nr1i1 22337 ADS9230 Intron 1 -22311 to -22303 1061 to 1069 Chr15:97907250-97907242 2 88 0.881 150
5 PCRs 2 genes 27 assays 105 CpGs 3,589 bp
NGS074 – Mouse Cdkn2a & Clca1
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N74P1 Cdkn2a p16 12578 ADS5218 Exon 1 37 to 126 167 to 256 Chr4:89294487-89294398 10 159 0.958 800
4-plex Cdkn2a p16 12578 ADS5226 Intron 1 12884 to 12941 13014 to 13071 Chr4:89281640-89281583 2 153 0.901 4,000
Ta = 59 Cdkn2a p16 12578 ADS5228 Intron 1 13231 to 13281 13361 to 13411 Chr4:89281293-89281243 2 105 0.983 2,400
Cdkn2a p16 12578 ADS5224 Intron 1 7509 to 7532 7639 to 7662 Chr4:89287015-89286992 3 95 0.986 4,500
N74P2 Cdkn2a p16 12578 ADS5217 5′-Upstream, 5′-UTR, Exon 1 -131 to 7 -1 to 137 Chr4:89294654-89294517 15 228 0.973 900
5-plex Cdkn2a p16 12578 ADS5231 Intron 2 19440 to 19583 19570 to 19713 Chr4:89275084-89274941 9 204 0.960 150
Ta = 56 Cdkn2a p16 12578 ADS5225 Intron 1 11946 to 12019 12076 to 12149 Chr4:89282578-89282505 4 131 0.961 2,200
Cdkn2a p16 12578 ADS5223 Intron 1 5579 to 5609 5709 to 5739 Chr4:89288945-89288915 2 111 0.985 1,700
Cdkn2a p16 12578 ADS5221 Intron 1 1287 1417 Chr4:89293237 1 75 0.978 500
N74P3 Clca1 Gob5 23844 ADS5256 Exon 1, Intron 1 90 to 264 121 to 295 Chr3:145032656-145032482 7 260 0.961 200
8-plex Clca1 Gob5 23844 ADS5252 Intron 1 3512 to 3634 3543 to 3665 Chr3:145029234-145029112 3 195 0.981 1,000
Ta = 55 Clca1 Gob5 23844 ADS5244 Exon 14 27676 to 27741 27707 to 27772 Chr3:145005070-145005005 3 186 0.970 100
Clca1 Gob5 23844 ADS5242 3′-UTR 28128 to 28143 28159 to 28174 Chr3:145004618-145004603 2 154 0.980 2,000
Clca1 Gob5 23844 ADS5258 5′-Upstream -2476 -2445 Chr3:145035221 1 144 0.996 700
Clca1 Gob5 23844 ADS5248 Exon 13, Intron 13 26828 to 26896 26859 to 26927 Chr3:145005918-145005850 6 125 0.982 3,000
Clca1 Gob5 23844 ADS5250 Exon 3 5357 5388 Chr3:145027389 1 124 0.989 1,800
Clca1 Gob5 23844 ADS5246 3′-UTR 28212 28243 Chr3:145004534 1 96 0.976 2,900
N74P4 Clca1 Gob5 23844 ADS5259 5′-Upstream -4720 to -4576 -4689 to -4545 Chr3:145037465-145037321 8 234 0.983 200
7-plex Clca1 Gob5 23844 ADS5251 Intron 1, Exon 2 4683 to 4818 4714 to 4849 Chr3:145028063-145027928 3 216 0.929 700
Ta = 55 Clca1 Gob5 23844 ADS5243 Exon 14 27886 to 27987 27917 to 28018 Chr3:145004860-145004759 5 189 0.987 400
Clca1 Gob5 23844 ADS5247 3′-Downstream 30730 to 30805 30761 to 30836 Chr3:145002016-145001941 3 179 0.983 200
Clca1 Gob5 23844 ADS5253 Intron 1 1480 to 1549 1511 to 1580 Chr3:145031266-145031197 2 159 0.983 1,000
Clca1 Gob5 23844 ADS5245 Intron 13 27563 27594 Chr3:145005183 1 145 0.984 1,800
Clca1 Gob5 23844 ADS5257 5′-Upstream -631 to -588 -600 to -557 Chr3:145033376-145033333 2 118 0.980 2,800
Simplex Ta = 59 Cdkn2a p16 12578 ADS5214 5′-Upstream -1242 to -1085 -1112 to -955 Chr4:89295765-89295608 9 223 0.909 2,500
Simplex Ta = 56 Cdkn2a p16 12578 ADS5215 5′-Upstream -636 to -522 -506 to -392 Chr4:89295159-89295045 17 184 0.846 1,200
6 PCRs 2 genes 26 assays 122 CpGs 4,192 bp
NGS004V1 – Mouse Foxp3 (forkhead box P3) Regulators
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (mm10) # CpGs Size (bp) RSQ Est. Cov.
N4V1P15 Ikzf4 Eos 22781 ADS3785 Intron 1 1554 to 1662 1738 to 1846 Chr10:128644254-128644146 5 210 0.971 700
6-plex Il2 Tcgf 16183 ASY548 5′-Upstream -308 to -263 -255 to -210 Chr3:37126214-37126169 2 188 0.988 3,500
Ta = 58 Foxp3 Jm2 20371 ADS443 Intron 1 -2405 to -2287 4275 to 4393 ChrX:7583950-7584068 6 181 0.951 300
Ikzf2 Helios 22779 ADS4020 Intron 3 26884 to 26961 28548 to 28625 Chr1:69657274-69657197 6 147 0.991 8,000
Foxp3 Jm2 20371 ADS779B Intron 1 -2145 to -2075 4535 to 4605 ChrX:7584210-7584280 2 144 0.981 6,000
Foxp3 Jm2 20371 ADS569B 5′-Upstream -12069 to -12010 -5390 to -5331 ChrX:7574286-7574345 15 113 0.947 800
N4V1P16 Tnfrsf18 Gitr 21936 ADS4018 Exon 5, 3′-UTR 2180 to 2262 2403 to 2485 Chr4:156028566-156028648 4 210 0.973 100
6-plex Ctla4 Cd152 12477 ADS3784B Exon 2 3302 to 3403 3448 to 3549 Chr1:60912472-60912573 4 161 0.987 700
Ta = 55 Tnf Tnfa 21926 ASY2219 5′-Upstream -1006 to -910 -839 to -743 Chr17:35202846-35202750 4 144 0.900 2,000
Foxp3 Jm2 20371 ADS442 Intron 1 -2555 to -2533 4125 to 4147 ChrX:7583800-7583822 2 129 0.975 300
Foxp3 Jm2 20371 ADS779A Intron 1 -2238 to -2207 4442 to 4473 ChrX:7584117-7584148 4 111 0.963 100
Foxp3 Jm2 20371 ASY1724 5′-Upstream -12859 to -12811 -6180 to -6132 ChrX:7573496-7573544 9 104 0.994 2,000
N4V1P17 Cd274 Pdl1 60533 ADS8832A 5′-Upstream -5104 to -5025 -91 to -12 Chr19:29367364-29367443 7 149 0.952 1,500
5-plex Il2ra Cd25 16184 ADS4019 Intron 1 2705 to 2738 2899 to 2932 Chr2:11645705-11645738 3 144 0.949 1,200
Ta = 52 Il4 Bsf-1 16189 ADS017B Intron 1 186 to 230 249 to 293 Chr11:53618421-53618377 3 129 0.985 7,000
Cdkn1c p57 12577 ADS4569 5′-UTR -350 to -275 33 to 108 Chr7:143461018-143460943 9 123 0.755 3,000
Foxp3 Jm2 20371 ADS1183 5′-Upstream -6750 to -6714 -71 to -35 ChrX:7579605-7579641 5 105 0.978 2,500
3 PCRs 11 genes 17 assays 90 CpGs 2,492 bp

Rat

NGS160 – Rat Pick1 (protein interacting with PRKCA 1)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (Rnor_6.0) # CpGs Size (bp) RSQ Est. Cov.
N160P1 Pick1 Prkcabp 84591 ASY3196 5′-Upstream -719 to -677 -311 to -269 Chr7:120464819-120464861 5 130 0.903 100
4-plex Pick1 Prkcabp 84591 ASY3318 Exon 4 9979 to 9995 10387 to 10403 Chr7:120475516-120475532 2 130 0.807 1,100
Ta = 51 Pick1 Prkcabp 84591 ASY3192 5′-Upstream -5174 to -5134 -4766 to -4726 Chr7:120460364-120460404 3 113 0.942 100
Pick1 Prkcabp 84591 ASY3197 5′-Upstream, 5′-UTR -454 to -396 -46 to 13 Chr7:120465084-120465142 11 110 0.959 300
N160P2 Pick1 Prkcabp 84591 ASY3319 Intron 1 -366 to -207 43 to 202 Chr7:120465172-120465331 12 238 0.910 100
5-plex Pick1 Prkcabp 84591 ASY3194 5′-Upstream -4213 to -4066 -3805 to -3658 Chr7:120461325-120461472 5 198 0.904 3,000
Ta = 56 Pick1 Prkcabp 84591 ASY3340 Intron 6 13468 to 13567 13876 to 13975 Chr7:120479005-120479104 7 180 0.561 2,200
Pick1 Prkcabp 84591 ASY3193 5′-Upstream -4983 to -4887 -4575 to -4479 Chr7:120460555-120460651 7 152 0.972 300
Pick1 Prkcabp 84591 ASY3195 5′-Upstream -1039 to -970 -631 to -562 Chr7:120464499-120464568 4 137 0.951 5,400
2 PCRs 1 gene 9 assays 56 CpGs 1,388 bp
NGS152 – Rat Fkbp5 (FKBP prolyl isomerase 5)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (Rnor_6.0) # CpGs Size (bp) RSQ Est. Cov.
N154P1 Fkbp5 Fkbp51 361810 ASY3126 Intron 8 37860 to 37917 37886 to 37943 Chr20:7981135-7981078 5 133 0.882 3,000
5-plex Fkbp5 Fkbp51 361810 ASY3061 Exon 5 24061 to 24099 24087 to 24125 Chr20:7994934-7994896 4 113 0.971 2,000
Ta = 59 Fkbp5 Fkbp51 361810 ASY3057 Intron 6 30296 to 30334 30322 to 30360 Chr20:7988699-7988661 3 104 0.832 1,500
Fkbp5 Fkbp51 361810 ASY3055 Intron 2 7464 to 7491 7490 to 7517 Chr20:8011531-8011504 3 98 0.878 200
Fkbp5 Fkbp51 361810 ASY3046 5′-Upstream -30758 to -30732 -30732 to -30706 Chr20:8049752-8049726 2 87 0.958 1,000
N154P2 Fkbp5 Fkbp51 361810 ASY3056 Intron 4 12731 to 12838 12757 to 12864 Chr20:8006264-8006157 5 193 0.978 100
4-plex Fkbp5 Fkbp51 361810 ASY3045 5′-Upstream -34288 to -34260 -34262 to -34234 Chr20:8053282-8053254 4 134 0.977 300
Ta = 61 Fkbp5 Fkbp51 361810 ASY3043 5′-Upstream -40095 to -40056 -40069 to -40030 Chr20:8059089-8059050 4 114 0.974 500
Fkbp5 Fkbp51 361810 ASY3125 Intron 7 35451 to 35464 35477 to 35490 Chr20:7983544-7983531 2 101 0.895 500
N154P3 Fkbp5 Fkbp51 361810 ASY3052 5′-Upstream -1187 to -1167 -1161 to -1141 Chr20:8020181-8020161 3 150 0.912 100
6-plex Fkbp5 Fkbp51 361810 ASY3054 Exon 2 3023 to 3065 3049 to 3091 Chr20:8015972-8015930 4 137 0.939 700
Ta = 59 Fkbp5 Fkbp51 361810 ASY3124 Intron 7 33544 to 33616 33570 to 33642 Chr20:7985451-7985379 8 130 0.819 200
Fkbp5 Fkbp51 361810 ASY3062 Intron 5 24778 to 24829 24804 to 24855 Chr20:7994217-7994166 3 112 0.962 500
Fkbp5 Fkbp51 361810 ASY3053 Exon 1 51 to 80 77 to 106 Chr20:8018944-8018915 2 105 0.979 600
Fkbp5 Fkbp51 361810 ASY3051 5′-Upstream -300 to -2977 -2974 to -2951 Chr20:8021994-8021971 3 97 0.922 700
N154P4 Fkbp5 Fkbp51 361810 ASY3127 Exon 9, Intron 9 39030 to 39183 39056 to 39209 Chr20:7979965-7979812 11 203 0.846 200
5-plex Fkbp5 Fkbp51 361810 ASY3048 5′-Upstream -23913 to -23859 -23887 to -23833 Chr20:8042907-8042853 6 137 0.908 1,500
Ta = 59 Fkbp5 Fkbp51 361810 ASY3064 Intron 5 29151 to 29200 29177 to 29226 Chr20:7989844-7989795 4 133 0.856 300
Fkbp5 Fkbp51 361810 ASY3058 Intron 4 13697 to 13727 13723 to 13753 Chr20:8005298-8005268 5 114 0.917 500
Fkbp5 Fkbp51 361810 ASY3049 5′-Upstream -20216 to -20174 -20190 to -20148 Chr20:8039210-8039168 4 99 0.921 900
N154P5 Fkbp5 Fkbp51 361810 ADS3141 5′-Upstream -164 to -137 -138 to -111 Chr20:8019158-8019131 5 160 0.991 100
3-plex Fkbp5 Fkbp51 361810 ASY3059 Intron 4 16686 to 16767 16712 to 16793 Chr20:8002309-8002228 6 151 0.926 500
Ta = 56 Fkbp5 Fkbp51 361810 ASY3060 Intron 4 20329 to 20365 20355 to 20931 Chr20:7998666-7998630 3 118 0.965 800
5 PCRs 1 gene 23 assays 99 CpGs 2,923 bp
NGS122 – Rat Vglut1, 2, & 3
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (Rnor_6.0) # CpGs Size (bp) RSQ Est. Cov.
N122P3 Slc17a8 Vglut3 266767 ADS9587 Intron 2 24601 to 24702 25201 to 25302 Chr7:30249784-30249683 3 198 0.985 60
5-plex Slc17a8 Vglut3 266767 ADS9582 5′-Upstream -1028 to -910 -428 to -310 Chr7:30275412-30275294 5 178 0.988 80
Ta = 59 Slc17a8 Vglut3 266767 ADS9585 Intron 1 1297 to 1370 1897 to 1970 Chr7:30273088-30273015 4 124 0.995 150
Slc17a8 Vglut3 266767 ADS9586 Intron 2 24186 to 24227 24786 to 24827 Chr7:30250199-30250158 4 104 0.986 500
Slc17a8 Vglut3 266767 ADS9584 5′-UTR -95 to -47 506 to 554 Chr7:30274479-30274431 4 99 0.986 100
N122P5 Slc17a6 Vglut2 84487 ADS9575 Intron 1 1930 to 2004 2473 to 2547 Chr1:107001095-107001169 4 151 0.992 500
6-plex Slc17a6 Vglut2 84487 ADS9577 Intron 2 3559 to 3639 4102 to 4182 Chr1:107002724-107002804 8 150 0.850 400
Ta = 59 Slc17a6 Vglut2 84487 ADS9579 Intron 3 4866 to 4929 5409 to 5472 Chr1:107004031-107004094 9 145 0.885 150
Slc17a6 Vglut2 84487 ADS9571 5′-Upstream -848 to -843 -305 to -300 Chr1:106998318-106998323 2 136 0.903 50
Slc17a6 Vglut2 84487 ADS9576 Intron 1 2555 to 2597 3098 to 3140 Chr1:107001720-107001762 3 110 0.997 750
Slc17a6 Vglut2 84487 ADS9580 Intron 3 4995 to 5018 5538 to 5561 Chr1:107004160-107004183 3 97 0.972 100
N122P6 Slc17a8 Vglut3 266767 ASY1512 5′-Upstream -4132 to -3997 -3532 to -3397 Chr7:30278516-30278381 6 222 0.951 50
5-plex Slc17a8 Vglut3 266767 ADS9583 5′-UTR -354 to -276 247 to 325 Chr7:30274738-30274660 4 165 0.980 80
Ta = 59 Slc17a8 Vglut3 266767 ADS9588 Intron 10 53803 to 53855 54403 to 54455 Chr7:30220582-30220530 3 154 0.994 100
Slc17a8 Vglut3 266767 ADS9581 5′-Upstream -1259 to -1195 -659 to -595 Chr7:30275643-30275579 4 124 0.990 200
Slc17a8 Vglut3 266767 ADS9589 Intron 10 54105 to 54126 54705 to 54726 Chr7:30220280-30220259 2 108 0.994 100
N122P7 Slc17a7 Vglut1 116638 ADS9563 Exon 12, 3′-UTR 10560 to 10708 10693 to 10841 Chr1:101171944-101172092 9 202 0.973 250
5-plex Slc17a7 Vglut1 116638 ADS9570 Intron 9 8317 to 8444 8450 to 8577 Chr1:101169701-101169828 5 195 0.982 100
Ta = 59 Slc17a7 Vglut1 116638 ASY1502 5′-Upstream -1589 to -1463 -1456 to -1330 Chr1:101159796-101159922 8 193 0.984 200
Slc17a7 Vglut1 116638 ADS9569 Intron 9 8200 to 8255 8333 to 8388 Chr1:101169584-101169639 5 128 0.950 100
Slc17a7 Vglut1 116638 ADS9567 Intron 8 7924 to 7956 8057 to 8089 Chr1:101169308-101169340 2 92 0.976 350
N122P8 Slc17a7 Vglut1 116638 ASY1501 5′-Upstream -2323 to -2210 -2190 to -2077 Chr1:101159062-101159175 7 175 0.981 100
4-plex Slc17a7 Vglut1 116638 ADS9561 Exon 12 10412 to 10431 10545 to 10564 Chr1:101171796-101171815 3 142 0.952 50
Ta = 59 Slc17a7 Vglut1 116638 ASY1500 5′-Upstream -3912 to -3869 -3779 to -3736 Chr1:101157473-101157516 3 103 0.983 350
Slc17a7 Vglut1 116638 ADS9562 Exon 12 10493 to 10533 10626 to 10666 Chr1:101171877-101171917 4 94 0.975 300
N122P10 Slc17a8 Vglut3 266767 ADS9590 Exon 12 57308 to 57431 57908 to 58031 Chr7:30217077-30216954 7 203 0.990 150
2-plex Ta = 51 Slc17a8 Vglut3 266767 ASY1513 5′-Upstream -1966 to -1877 -1366 to -1277 Chr7:30276350-30276261 5 149 0.978 3,300
N122P12 Slc17a7 Vglut1 116638 ADS9568 Exon 9 8039 to 8154 8172 to 8287 Chr1:101169423-101169538 10 181 0.952 150
2-plex Ta = 59 Slc17a7 Vglut1 116638 ADS9566 Exon 7 6864 to 6936 6997 to 7069 Chr1:101168248-101168320 5 154 0.966 250
N122P13 Slc17a6 Vglut2 84487 ADS9572 5′-Upstream -797 to -724 -254 to -181 Chr1:106998369-106998442 9 134 0.938 400
3-plex Slc17a6 Vglut2 84487 ADS9573 5′-UTR -385 to -316 159 to 228 Chr1:106998781-106998850 6 126 0.991 150
Ta = 50 Slc17a6 Vglut2 84487 ADS9578 Intron 2 4154 to 4189 4697 to 4732 Chr1:107003319-107003354 3 90 0.987 80
Simplex Ta = 56 Slc17a6 Vglut2 84487 ADS9574 5′-UTR, Exon 1 -96 to 32 448 to 575 Chr1:106999070-106999197 6 181 0.989 300
9 PCRs 3 genes 33 assays 165 CpGs 4,807 bp

Monkey

NGS147 – Rhesus Monkey MLH1 (mutL homolog 1)
PCR ID Gene Alias NCBI ID Assay ID Location From ATG From TSS Coordinates (Mmul_10) # CpGs Size (bp) RSQ Est. Cov.
N147P1 MLH1 COCA2 698782 ADS8979 5′-Upstream -1718 to -1647 -1657 to -1586 Chr2:92412718-92412789 2 215 0.971 650
7-plex MLH1 COCA2 698782 ADS8976 5′-Upstream -2833 to -2751 -2772 to -2690 Chr2:92411603-92411685 2 189 0.987 250
Ta = 59 MLH1 COCA2 698782 ASY2818 5′-Upstream -1250 to -1138 -1189 to -1077 Chr2:92413186-92413298 4 183 0.997 250
MLH1 COCA2 698782 ASY2834 Intron 1 494 to 584 555 to 645 Chr2:92414929-92415019 2 182 0.992 600
MLH1 COCA2 698782 ASY2815 5′-Upstream -5154 to -5040 -5094 to -4979 Chr2:92409282-92409396 3 176 0.968 3,000
MLH1 COCA2 698782 ASY2836 Intron 1 848 to 895 909 to 956 Chr2:92415283-92415330 2 167 0.983 1,700
MLH1 COCA2 698782 ASY2833 Intron 1 371 to 382 432 to 443 Chr2:92414806-92414817 2 85 0.975 250
N147P2 MLH1 COCA2 698782 ASY2831 Intron 1 120 to 316 181 to 377 Chr2:92414555-92414751 21 255 0.984 60
7-plex MLH1 COCA2 698782 ADS8977re 5′-Upstream -2411 to -2226 -2350 to -2165 Chr2:92412025-92412210 4 248 0.991 1,000
Ta = 59 MLH1 COCA2 698782 ASY2828 5′-Upstream -742 to -568 -681 to -507 Chr2:92413694-92413868 26 243 0.726 300
MLH1 COCA2 698782 ADS8982 5′-Upstream -1061 to -947 -1000 to -886 Chr2:92413375-92413489 6 207 0.992 150
MLH1 COCA2 698782 ASY2835 Intron 1 692 to 726 753 to 787 Chr2:92415127-92415161 3 176 0.843 70
MLH1 COCA2 698782 ADS8978re 5′-Upstream -1869 -1808 Chr2:92412567 1 163 0.996 600
MLH1 COCA2 698782 ADS8984 5′-Upstream -130 to -62 -69 to -1 Chr2:92414306-92414374 6 124 0.793 1,250
N147P3 MLH1 COCA2 698782 ADS8980re 5′-Upstream -1479 to -1409 -1418 to -1348 Chr2:92412957-92413027 4 243 0.993 650
5-plex MLH1 COCA2 698782 ADS8985 5′-Upstream, 5′-UTR, Exon 1 -62 to 79 -1 to 140 Chr2:92414374-92414514 14 182 0.993 250
Ta = 56 MLH1 COCA2 698782 ASY2830 5′-Upstream -310 to -199 -249 to -138 Chr2:92414126-92414237 9 176 0.968 3,800
MLH1 COCA2 698782 ASY2817 5′-Upstream -3205 to -3160 -3144 to -3099 Chr2:92411231-92411276 2 136 0.985 70
MLH1 COCA2 698782 ASY2829 5′-Upstream -443 to -363 -382 to -302 Chr2:92413993-92414073 6 134 0.973 1,800
3 PCRs 1 gene 19 assays 118* 3,484 bp

Other

NGS109 – Human Mitochondrial Genome
PCR ID Gene Alias NCBI ID Assay ID Coordinates (hg38) # CpGs Size (bp) RSQ Est. Cov.
N109P1 ATP6 n/a 4508 ADS5995 ChrM:8647-8781 3 200 0.976 900
8-plex ATP8 n/a 4509 ADS5996 ChrM:8532-8583 4 146 0.973 200
Ta = 53 CO1 COX1 4512 ADS5976 ChrM:6445-6540 4 145 0.982 1,500
NCR n/a n/a ADS9413 ChrM:16411-16495 5 141 0.891 1,500
CYB CYTB 4519 ADS5980 ChrM:15349-15436 5 138 0.932 3,500
ND1 n/a 4535 ADS5972 ChrM:4126-4152 4 113 0.969 600
NCR n/a n/a ADS5997 ChrM:16328-16360 2 110 0.830 500
CO1 COX1 4512 ADS5987 ChrM:7196-7235 6 96 0.938 600
N109P2 CO2 COX2 4513 ADS5988 ChrM:7756-7858 7 193 0.954 100
6-plex ND2 n/a 4536 ADS5971 ChrM:4375-4463 4 172 0.970 900
Ta = 55 ND1 n/a 4535 ADS9515 ChrM:3351-3459 8 168 0.936 100
RNR1 n/a 4549 ADS9416 ChrM:1473-1561 6 152 0.881 60
CYB n/a 4519 ADS5981 ChrM:15199-15275 2 151 0.948 400
ND5 n/a 4540 ADS5957 ChrM:12862-12888 5 101 0.881 600
N109P3 CYB CYTB 4519 ADS5953 ChrM:15499-15616 6 178 0.970 200
9-plex ATP6 n/a 4508 ADS5985 ChrM:8958-9054 5 166 0.987 400
Ta = 51 ATP6 n/a 4508 ADS5986 ChrM:8855-8878 2 161 0.964 50
CYB CYTB 4519 ADS2886 ChrM:14920-15004 8 150 0.956 600
ND3 n/a 4537 ADS5963 ChrM:10142-10202 7 149 0.959 1,000
ATP6 n/a 4508 ADS5984 ChrM:9138-9195 4 140 0.947 350
ND1 n/a 4535 ADS5974 ChrM:3642-3706 5 139 0.940 1,000
CO3 COX3 4514 ADS5966 ChrM:9752-9827 6 136 0.917 1,000
CO1 COX1 4512 ADS5975 ChrM:6164-6189 4 90 0.948 300
N109P4 CO2 COX2 4513 ADS5994 ChrM:7599-7663 3 157 0.933 70
4-plex ND5 n/a 4540 ADS5955 ChrM:13524-13609 6 155 0.938 2,500
Ta = 55 ND5 n/a 4540 ADS5956 ChrM:13179-13239 4 121 0.868 800
ND4 n/a 4538 ADS5961 ChrM:11162-11191 3 116 0.960 1,000
N109P5 RNR1 n/a 4549 ADS9500 ChrM:624-785 5 246 0.869 70
7-plex ND5 n/a 4540 ADS5983 ChrM:12735-12817 5 194 0.885 800
Ta = 51 ND4L n/a 4539 ADS5962 ChrM:10670-10774 5 190 0.887 600
ND5 n/a 4540 ADS5992 ChrM:13702-13809 5 170 0.861 500
ND2 n/a 4536 ADS5970 ChrM:4620-4712 4 169 0.972 900
RNR2 n/a 4550 ADS9417 ChrM:1905-2001 5 155 0.851 70
CO1 COX1 4512 ADS5978 ChrM:7418-7462 3 124 0.854 100
N109P6 ND5 n/a 4540 ADS5991 ChrM:13287-13405 4 204 0.893 2,500
5-plex ND4 n/a 4538 ADS5959 ChrM:11647-11777 8 191 0.978 1,000
Ta = 60 ND6 n/a 4541 ADS2887 ChrM:14159-14267 4 183 0.849 200
CO3 COX3 4514 ADS5965 ChrM:9911-10013 4 166 0.925 3,800
CO3 COX3 4514 ADS5989 ChrM:9574-9620 3 130 0.963 400
N109P7 ND2 n/a 4536 ADS5968 ChrM:5236-5399 5 236 0.959 250
5-plex RNR1 n/a 4549 ADS9415 ChrM:1177-1322 7 217 0.864 200
Ta = 51 ND4 n/a 4538 ADS5958 ChrM:12053-12206 5 210 0.884 250
ND4 n/a 4538 ADS5960 ChrM:11389-11492 6 204 0.932 70
ND2 n/a 4536 ADS5969 ChrM:5053-5163 4 203 0.970 400
N109P8 CO1 COX1 4512 ADS5979 ChrM:6617-6689 3 144 0.949 250
8-plex ND5 n/a 4540 ADS5954 ChrM:13914-13967 3 134 0.925 1,500
Ta = 55 CO1 COX1 4512 ADS9424 ChrM:6053-6128 4 132 0.842 50
ND2 n/a 4536 ADS5967 ChrM:5459-5470 2 130 0.883 1,400
RNR2 n/a 4550 ADS9419 ChrM:2642-2718 4 129 0.920 2,500
ND6 n/a 4541 ADS2921A ChrM:14383-14458 2 127 0.952 600
CO1 n/a 4512 ADS9425 ChrM:6998-7019 4 127 0.901 200
NCR n/a n/a ADS5964 ChrM:61-120 7 114 0.863 1,500
8 PCRs 15 genes 52 assays 239 CpGs 8,113 bp

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