Global DNA Methylation

Service Description

Pyrosequencing can be used to determine the average amount of DNA methylation within a sample. Nearly half (45%) of the mammalian genome is constituted of repetitive elements (such as the LINE/Alu sequences in humans). As a result, examining these repetitive segments has become a standard method for determining global DNA methylation levels.

EpigenDx uses direct Pyrosequencing following bisulfite conversion of DNA to analyze methylation. All of our assays are pre-validated to ensure that neither methylation nor unmethylated DNA is amplified preferentially, allowing us to provide you with the best quality data and results.

line-1 global DNA methylation flyer

Download LINE-1 Flyer

Step One

Select a Global DNA Methylation Marker

Human Global Methylation
  • Human LINE-1 Element
  • Human Alu Elements
  • Human SAT2 Element
  • Human HERVE LTR Elements
Mouse Global Methylation
  • Mouse LINE-1 Element
  • Mouse B1 Element
  • Mouse IAP Element

Rat Global Methylation
  • Rat LINE-1 Element
  • Rat ID Element
  • Rat Satellite-I

Step Two

Choose an Ideal Plan for You

All parts of our methylation services are customizable and modular. Pick and choose the service plan that is appropriate and ideal for your needs.

Item No. Service Service Description Price
8001 STANDARD

DNA Methylation Analysis

 Quantitative Methylation Analysis on submitted bisulfite-converted PCR products

  • Perform Pyrosequencing run & methylation analysis ONLY
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8002 Standard Plus

DNA Methylation Analysis

 Quantitative Methylation Analysis

  • Bisulfite PCR using analytically validated assay
  • Pyrosequencing sample preparation: making ssDNA template for Pyrosequencing
  • Setup Pyrosequencing, and run Pyrosequencing
  • Pyrosequencing run QC: Pass E & S, negative dispensation, incomplete bisulfite modification, and correct sequence pattern
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Turn-around time is 5-15 days for most DNA methylation services, depending the number of assays and samples sent. Click here for a detailed breakdown of EpigenDx’s methylation service workflow.

Step Three | Results

EpigenDx analyzes the raw data and delivers:

  •   The percentage methylation values of each CpG site in a Microsoft Excel spreadsheet.
  •   The raw pyrograms in a PDF document.

Interpretation of Data

Methylation data can then be applied to research. For example, normal healthy individuals maintain high methylation levels (75-85%) in the LINE-1 elements to suppress retrotransposons that would otherwise copy and transpose themselves erratically across the genome. Abnormally low methylation in the LINE elements have been shown to be common in many malignancies, due to genomic instability.

Figure 1: Methylation Average of 1892 Normal Purified Blood LINE1 Samples

EpigenDx - Methylation Average of 1892 Normal Purified Blood LINE1 Samples
Speak with experts at EpigenDx for high quality technical support and consultation through all phases of the project, from assay design to data delivery.

Experts in DNA Methylation Analysis

  •   15+ years of experience
  •   Measure the percent methylation of any CpG site in any target region
  •   Targeted bisulfite sequencing
  •   Pre-validated assays covering CpG-rich regions
  •   High, low, and premix methylation controls
  •   Comprehensive data validation and analysis

EpigenDx

Experts in DNA Methylation

Working With Us Is Simple

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LUMA Assay service

LUMA Assay

(LUminometric Methylation Assay) — A high throughput method to the analysis of genomic DNA methylation. The LUMA assay could be beneficial for analyzing genome-wide DNA methylation in a variety of physiological and pathological situations, such as cancer etiology, diagnosis, and prognosis. Changes in genomic DNA methylation are known to have a role in both normal and pathological processes, contributing to both normal development and differentiation as well as cancer and other illnesses. Read Abstract.

For non-standards:

  •   Birds
  •   Fish
  •   Others
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EpigenDx Offers GLP and GCP Compliant Laboratory Services & Many Other Customized Services
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