DNA Methylation Analysis

Targeted Bisulfite Sequencing for DNA Methylation Analysis

EpigenDx specializes in targeted bisulfite next-generation sequencing (NGS) services for DNA Methylation Analysis with affordable, fast, and precise sequencing of custom target regions. Our targeted bisulfite sequencing services can provide methylation data for dozens of genes and entire promoter regions in a single run.

Choose from pre-validated panels (human, mouse or rat) or utilize our design services to develop a custom library covering your targets of interest. All of our off-the-shelf panels are validated by using Pyrosequencing technology. We use Ion Torrent™ technology for fast and accurate targeted gene sequencing.

Custom NGS Assay Design

Targeted NGS Analysis

NGS Data Analysis

» Loci-specific Methylation

Analysis of the percentage methylation levels of all consecutive CpG sites within a gene locus.

  • Targeted bisulfite NGS
  • Pyrosequencing

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» Global Methylation

Determine the average amount of DNA methylation within a sample

  • Transposable elements: Line-1 | Alu | LTR

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DNA Methylation Services

Item No. Service Description Price
8200 CUSTOM

NGS Assay Design

 Assay design based on customer-submitted target region(s).

  • Includes the cost of oligos
 Inquire
8500

STANDARD

Targeted Next-Gen Sequencing

 Custom target gene sequencing using Ion Torrent instruments:

  • Customer provides the PCR amplicons
  • EpigenDx constructs the library and performs Next-Gen Sequencing
 Inquire
8501

Premium

Targeted Next-Gen Sequencing

 Custom target gene sequencing using Ion Torrent instruments:

  • Customer provides tissue samples or isolated DNA
  • EpigenDx performs:
    • DNA extraction
    • Bisulfite modification
    • Multiplex PCR
    • Library construction
    • Next-Gen Sequencing
 Inquire
8100a NGS Data Analysis Preliminary data analysis of NGS generated sequencing data. Inquire

Advantages

Targeted Next-Gen Bisulfite Sequencing is more economical and has a faster turnaround time than whole-genome methylation analysis (WGBS or RRBS). It allows you to analyze only the regions and genes you are interested in and produces higher quality data due to significantly greater coverage of each CpG site.

EpigenDx, with over 15 years of experience in bisulfite sequencing, offers a complete solution for targeted NGS. We perform the DNA extraction, bisulfite modification, library preparation, high throughput sequencing, data analysis, and assay validation with Pyrosequencing.

Over 100 Pre-Validated Panels!

Applications

DNA Methylation Analysis

  • Measure the percent methylation of multiple target regions and multiple samples in a single run.
  • Ability to perform DNA methylation analysis covering many areas of one gene or a diverse set of regions across multiple genes

Infinium MethylationEPIC Assay Validation

Pre-Designed Methylation Panels

Use our pre-validated off-the-shelf methylation panels to assess the methylation levels of genes related to cancers, diseases, immunology, pluripotency, and more.

Custom Panel
Design

Send us custom target regions with your genes of interest. We will design and validate a custom panel of assays.

Ready To Get Started On Your Project?

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Contact Us

EpigenDx, Inc.

 Tel. (508) 497-9400 Free (800) 941-6884 info@epigendx.com