Analysis of the percentage methylation levels of all consecutive CpG sites within a gene locus.
Since DNA methylation is frequently heterogeneous within a sample, it is important to quantify the relative proportion of methylated and unmethylated alleles at each CpG site. We achieve this by direct Pyrosequencing after bisulfite modification and PCR amplification.
All of our assays are pre-validated to ensure there is no preferential amplification for either methylated or unmethylated DNA, allowing us to provide the highest quality data and results. EpigenDx has almost 15 years of expertise in epigenetics using Pyrosequencing technology.