Analysis of the percentage methylation levels of all consecutive CpG sites within a gene locus. Since DNA methylation is frequently heterogeneous within a sample, it is important to quantify the relative proportion of methylated and unmethylated alleles at each CpG site. We achieve this by direct Pyrosequencing after bisulfite modification and PCR amplification. All of our assays are pre-validated to ensure there is no preferential amplification for either methylated or unmethylated DNA, allowing us to provide the highest quality data and results. EpigenDx has almost 15 years of expertise in epigenetics using Pyrosequencing technology.
Loci-Specific DNA Methylation
Step One | Selecting a Pyrosequencing Target
Figure 1: Promoter Region of Human GCR (NR3C1) (Ensembl Transcript: ENST00000231509)
Description: This is an example of EpigenDx’s CpG site coverage in a typical gene such as Human GCR. Red markers are CpG sites covered by at least one of EpigenDx’s pre-developed assays (ADS). Blue X’s are all other CpG sites.
Step Two | Choose an Ideal Plan for You
All parts of our methylation services are customizable and modular. Pick and choose the service plan that is appropriate and ideal for your needs.
DNA Methylation Analysis
|Quantitative Methylation Analysis on submitted bisulfite-converted PCR products
DNA Methylation Analysis
|Quantitative Methylation Analysis
Step Three | Results
EpigenDx analyzes the raw data and delivers:
- The percentage methylation values of each CpG site in a Microsoft Excel spreadsheet.
- The raw pyrograms in a PDF document.
Interpretation of Data
Methylation data can then be applied to research. For example, normal healthy individuals maintain high methylation levels (75-85%) in the LINE-1 elements to suppress retrotransposons that would otherwise copy and transpose themselves erratically across the genome. Abnormally low methylation in the LINE elements have been shown to be common in many malignancies, due to genomic instability.
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Can’t Locate Your Region Of Interest?
No problem, we’ve got you covered. EpigenDx provides custom assay design and analytical services with an assay database of thousands of Off-the-Shelf designs ready to go. Not only do we provide aggressive timelines with regular project updates, you will also get the highest quality technical support and consultation from design phase through project completion and data delivery.
Experts in DNA Methylation