Methylation Controls

EpigenDx Human_Methylated Standard Products

Scientific Overview

EpigenDx manufactures methylated genomic DNA controls. They are intended to serve as positive or negative controls in DNA methylation analysis, and are available for human, mouse, rat, and monkey DNA methylation analysis assays.

Our highly methylated DNA is in vitro enzymatically methylated genomic DNA with greater than 85% methylation. Our low methylated DNA is chemically and enzymatically treated with lower than 5% methylation. They are tested on numerous gene-specific and global methylation assays using pyrosequencing technology.

Methylation Control Data

Control DNAs are useful in the study of methylation in genomic DNA and epigenomic studies. They can be used in a variety of methods to act as comparisons to sample DNA. Bisulfite conversion techniques, methyl-DNA immunoprecipitation (Me-DIP), methyl-CpG binding domain-based (MBDCap) proteins, methylation-specific PCR (MSP), and real-time PCR all have positive and negative methylation control DNA available.

Prior to proceeding with downstream studies, DNA and RNA quantification, also known as nucleic acid quantification, is typically conducted to establish the average concentration of DNA or RNA in a sample.

High Methylated Control gDNA (Mouse LINE1 Assay)

high_methylated_control_chart

Low Methylated Control gDNA (Mouse LINE1 Assay)

low_methylated_control_chart

Product Details

Product Contents:
HIGH Methylation Control
(Human/Mouse/Rat/Monkey)

  • 1 vial High Methylated Genomic DNA (5 µg at 100 ng/µL, > 85% Methylation)

Product Contents:
LOW Methylation Control
(Human/Mouse/Rat/Monkey)

  • 1 vial Low Methylated Genomic DNA (5 µg at 100 ng/µL, < 5% Methylation)

Product Contents:
Pre-mixed Calibration Standard
(Human/Mouse/Rat/Monkey)

  • 1 vial 0% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 5% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 10% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 25% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 50% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 75% Methylated Genomic DNA (1 µg at 50 ng/µL)
  • 1 vial 100% Methylated Genomic DNA (1 µg at 50 ng/µL)

Product Information

Item Description Notes Price Order
80-8060 Custom made DNA controls:  Low or high mentholated DNA, 5µg yield. Custom made DNA methylation controls by submitting greater than 10 µg of genomic DNA.  $632.50 Inquire
80-8060H-bsMix Human bisulfite modified genomicl DNA  controls including 15 ul of each low, medium, and high methylated DNAs Ready to use human methylation controls for bisulfite sequencing, Pyrosequencing, targeted bisulfite NGS, MS-HRM, EPIC array, RRBS, WGBS  $591.77 Inquire
80-8060H-PreMix Human Pre-Mixed  Methylation Controls at different ratios: Low methylated DNA (0%), 10%, 25%, 50%, 75%, 90%, and high methylated DNA (100%). Premixed levels of Human High and Low methylation control DNA can be used to quantify the methylation levels of unknown samples or to validate quantitative methylation assays.  $382.82 Inquire
80-8060M-PreMix Moue Pre-Mixed Methylation Controls at different ratios: Low methylated DNA (0%), 10%, 25%, 50%, 75%, 90%, and high methylated DNA (100%). Premixed levels of Mouse High and Low methylation control DNA can be used to quantify the methylation levels of unknown samples or to validate quantitative methylation assays.  $389.30 Inquire
80-8060PM-PreMix Monkey Pre-Mixed Methylation Controls at different ratios: Low methylated DNA (0%), 10%, 25%, 50%, 75%, 90%, and high methylated DNA (100%). Premixed levels of monkey (Rhesus) High and Low methylation control DNA can be used to quantify the methylation levels of unknown samples or to validate quantitative methylation assays.  $409.46 Inquire
80-8060R-PreMix Rat Pre-Mixed Methylation Controls at different ratios: Low methylated DNA (0%), 10%, 25%, 50%, 75%, 90%, and high methylated DNA (100%). Premixed levels of rat High and Low methylation control DNA can be used to quantify the methylation levels of unknown samples or to validate quantitative methylation assays.  $389.30 Inquire
80-8061-HGHM5 Human Controls: High Methylated DNA Control Human High Methylated Genomic DNA is derived from whole blood and in vitro enzymatically methylated resulting in greater than 85% methylation  $237.92 Inquire
80-8062-HGUM5 Human Controls: Low Methylated DNA Control Human Low Methylated Genomic DNA is derived from whole blood and chemically and enzymatically methylated resulting in less than 10% methylation  $237.92 Inquire
80-8063-MGHM5 Mouse Controls: High Methylated DNA Control Mouse High Methylated Genomic DNA is derived from whole blood and in vitro enzymatically methylated resulting in greater than 85% methylation  $248.73 Inquire
80-8064-MGUM5 Mouse Controls: Low Methylated DNA Control Mouse Low Methylated Genomic DNA is derived from whole blood and chemically and enzymatically methylated resulting in less than 10% methylation  $248.73 Inquire
80-8065-RGHM5 Rat Controls: High  Methylated DNA Control Rat High Methylated Genomic DNA is derived from whole blood and in vitro enzymatically methylated resulting in greater than 85% methylation  $248.73 Inquire
80-8066-RGUM5 Rat Controls: Low Methylated DNA Control Rat Low Methylated Genomic DNA is derived from whole blood and chemically and enzymatically methylated resulting in less than 10% methylation  $248.73 Inquire
80-8067-PMGHM5 Monkey (Rhesus) high methylated genomic DNA Monkey High Methylated Genomic DNA is derived from whole blood and in vitro enzymatically methylated resulting in greater than 85% methylation  $275.63 Inquire
80-8068-PMGUM5 Monkey (Rhesus) low methylated genomic DNA Monkey Low Methylated Genomic DNA is derived from whole blood and chemically and enzymatically methylated resulting in less than 10% methylation  $275.63 Inquire
80-8069-LHM5 Bacteriophage lambda high methylated genomic DNA In vitro enzymatically methylated bacteriophage lambda DNA with greater than 85% methylation.  Ideal controls for LUMA Pyrosequencing analysis.  $200.12 Inquire
80-8070-LLM5 Low methylation controls for LUMA analysis In vitro enzymatically demethylated bacteriophage lambda DNA with less than 5% methylation.  Ideal controls for LUMA Pyrosequencing analysis.  $200.12 Inquire
80-8081-HGMM5 Human Controls: Medium Methylation DNA genomic DNA Human methylation controls at 50% +/- 15% of methylation  $297.40 Inquire
80-8082-MGMM5 Mouse Controls: Medium Methylation DNA genomic DNA Mouse methylation controls at 50% +/- 15% of methylation  $310.92 Inquire
80-8083-RGMM5 Rat Controls: Medium Methylation DNA genomic DNA Rat methylation controls at 50% +/- 15% of methylation  $310.92 Inquire
80-8084-PMGMM5 Monkey Control: Medium Methylation DNA genomic DNA Monkey methylation controls at 50% +/- 15% of methylation  $344.54 Inquire
80-8090 Custom made pre-mixed methylation at different ratios: Low methylated DNA (0%), 10%, 25%, 50%, 75%, 90%, and high methylated DNA (100%). Custom made pre-mixed DNA methylation controls at different ratios by submitting greater than 10 µg of genomic DNA.  $782.93 Inquire

Experts Since 2006

Custom Panel Design

With over 15 years of experience, EpigenDx offers customizable DNA Methylation analysis for specific gene targets of your interest. We offer rapid turnaround time for clinical trials and molecular diagnosis as well as MGMT/Line-1/Foxp3 tests for HRM realtime PCR, Pyrosequencing, and targeted bisulfite NGS. Simply send us custom target regions with your genes of interest. We will design and validate a panel of assays suited to your research interests.

EpigenDx

Experts in DNA Methylation

CpG Dinucleotides

The human genome contains ∼30,000 CpG islands (CGIs), long stretches (0.5–2 kb) of DNA with unusually elevated levels of CpG dinucleotides. In mammalian genomes, DNA methylation occurs at CpG dinucleotides and is a dynamic epigenetic control mechanism. At these locations, methylated CpG dinucleotides can cause a high rate of C to T substitution. It is well known that methylated CpG sites have a high mutation rate.

Whole-Genome Bisulfite Sequencing (WGBS)

Bisulfite sequencing (BS-Seq), also known as whole-genome bisulfite sequencing (WGBS), is a well-known method for detecting methylated cytosines in genomic DNA. Studies have shown that WGBS can be used to detect abnormal methylation in cancers such as acute promyelocytic leukemia, gastric cancer, and others by screening for specific hyper-methylated suppressor genes.

DNA Methyltransferase

DNA methyltransferases (DNMTs) are a group of “writer” enzymes that add a methyl group to the carbon atom number five (C5) of cytosine to cause DNA methylation. EpigenDx’s Stem Cell Panel covers 790 CpG sites within 33 human genes, including pluripotency-associated genes, DNA methyltransferases, imprinted genes, genes involved in X-chromosome inactivation, and cell differentiation regulators.

Learn more about our GLP/GCP Compliant Laboratory Services & Customized Services