Compared to other genotyping methods and platforms, pyrosequencing technology offers the unique advantage of providing quantitative measurements of every allele. This property makes Pyrosequencing the premier choice for allele-specific expression analysis, loss of heterozygosity, and studies involving mixed populations and sample pools.
The amplification signal in Pyrosequencing is proportional to the amount of genetic material and nucleotide present in the order of the sequence. As a result, it is possible to quantify allelic variation when the amplification signals are compared to reference peaks. Ideally, their sum would equal the signal level of the reference.