Validated Methylation Assay Kits

Scientific Overview

One of the most significant challenges in PCR design of bisulfite-converted genomic DNA is the design and optimization of primers to reduce PCR bias. When aiming to preserve the quantitative ratio of methylated-to-unmethylated CpG sites for quantitative methylation, it is imperative that the primer design be un-biased so that it will not select for one type of fragment over another.

Over 15,000 Off-the-Shelf Validated DNA Methylation Assays.

Kits available for Human, Mouse, Rat, Monkey, and more.

Compatible with:

  • MS-HRM
  • Pyrosequencing
  • Targeted Bisulfite NGS
  • Bisulfite Sequencing
human foxp3 methylation kit

Product Information

Item No. Description Genes Price
80-8080-ADS Validated Methylated Analysis Assay Kit Indicate desired target gene(s) Inquire

Product Content:

  •  DNA Methylation Assay Kits good for 100 Reactions*
  •  Ready for PCR amplification of bisulfite converted DNA with high efficiency
human MGMT methylation kit

Product Details

The nature of methylation analysis is that methylated and unmethylated DNA molecules will be amplified with greatly differing efficiencies. This is due to secondary structure formation (primer loops and dimers, template loops, mispriming) and long strings of T’s and G’s and C/T variation at CpG sites. Primers situated on CpG sites can selectively amplify either the methylated or unmethylated fragment, possibly producing bias in the final methylation analysis.

Assay Design & Validation

EpigenDx has expertise in PCR and assay design, and we validate the assay conditions using quantitative Pyrosequencing methylation technology to ensure methylated and unmethylated DNA are amplified at the same efficiency. We provide complete primer sets and assay conditions to our customers, optimized for bisulfite sequencing and other DNA methylation applications such as COBRA and HRM real-time PCR.


Experts in DNA Methylation

EpigenDx Offers GLP and GCP Compliant Laboratory Services