Bisulfite Pyrosequencing

EpigenDx Bisulfite Pyrosequencing

Pyrosequencing Technology for Methylation Analysis

Over 15,000+ Validated Assays Offered. EpigenDx is the world-wide leading Pyrosequencing service provider in the biotechnology industry. Our lab has nearly fifteen years of expertise in Pyrosequencing technology and assay design. We offer quality services, excellent customer support, and rapid turnaround time. Our design team is skilled in working with even the most difficult regions for epigenomic applications.

Services Include:
DNA Methylation Analysis
8001 | 8002
Bisulfite modification
8005
Assay Design
8006 | 8008

A Real-Time and Quantitative Solution

Pyrosequencing is EpigenDx’s choice platform for loci-specific DNA methylation analysis since it is fast, cost-effective, and can accurately provide real-time quantitative percent methylation reads. This makes it ideal for applications involving allele quantification. A single Pyrosequencing run for DNA methylation is equivalent to sequencing 20 clones with traditional Sanger Bisulfite Sequencing.

Loci-Specific DNA Methylation

Measure the percent methylation levels of all individual CpG sites in a target region (PCR amplicon).
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Global DNA Methylation

Assess the average amount of DNA methylation globally in a sample.
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Allele-Specific Quantitation

Quantify the amounts of two alleles at a basepair resolution.
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SNP Genotyping

SNP and mutation genotyping via Pyrosequencing technology.
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Custom Assay Design

Send us a custom target region with your genes of interest. We will design and validate the assay.
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Available Services:

Item No. Service Description Price
8001 STANDARD

DNA Methylation Analysis

 Quantitative Methylation Analysis on submitted bisulfite-converted PCR products

  • Perform Pyrosequencing run & methylation analysis ONLY
 Inquire
8002 Standard Plus

DNA Methylation Analysis

 Quantitative Methylation Analysis

  • Bisulfite PCR using analytically validated assay
  • Pyrosequencing sample preparation: making ssDNA template for Pyrosequencing
  • Setup Pyrosequencing, and run Pyrosequencing
  • Pyrosequencing run QC: Pass E & S, negative dispensation, incomplete bisulfite modification, and correct sequence pattern
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8005 Bisulfite modification Bisulfite modification on submitted genomic DNA

  • Process extracted genomic DNA from different sample sources, such as paraffin embedded tissues, frozen tissues, serum, and more
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8006 STANDARD

Assay Design

 Pyrosequencing primer design for DNA methylation, SNP genotyping, and allele specific expression analysis:

  • Customer provides target sequences
  • Customer designs PCR primers
  • EpigenDx determines validity of the PCR primers, designs Pyrosequencing primers, and determines the biotin labeled PCR primers.
 Inquire
8008 Premium

Assay Design

 Design and optimize PCR and Pyrosequencing primers for DNA methylation analysis, SNP genotyping, and allele specific expression analysis:

  • Design PCR and Pyrosequencing primers
  • Develop and optimize PCR conditions
  • Develop and optimize Pyrosequencing conditions
  • Perform assay validation
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EpigenDx Offers Many Custom Solutions and GLP and GCP Compliant Laboratory Services
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