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Search Validated Methylation Assays

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👨🏼 HUMAN

Assay Name	PCR ID	Gene	Alias	NCBI ID	Assay	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.

NGS162 - Human ANO1 (anoctamin 1)	PCR ID	Gene	Alias	NCBI ID	Assay	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N162P1	ANO1	TMEM16A	55107	ASY3379	Intron 9	46019 to 46108	46324 to 46413	Chr11:70124625-70124714	5	171	0.612	800
	5-plex	ANO1	TMEM16A	55107	ASY3389	5'-Upstream	-2559 to -2491	-2254 to -2186	Chr11:70076048-70076116	2	152	0.909	200
	Ta = 65	ANO1	TMEM16A	55107	ASY3316	Intron 1	556 to 642	861 to 947	Chr11:70079162-70079248	4	142	0.856	600
		ANO1	TMEM16A	55107	ASY3310	5'-Upstream	-735 to -667	-430 to -362	Chr11:70077872-70077940	6	138	0.946	3100
		ANO1	TMEM16A	55107	ASY3315	Intron 1	445 to 484	750 to 789	Chr11:70079051-70079090	4	97	0.729	1200
	N162P2	ANO1	TMEM16A	55107	ASY3378	Exon 6, Intron 6	29769 to 29885	30074 to 30190	Chr11:70108375-70108491	6	168	0.851	1300
	9-plex	ANO1	TMEM16A	55107	ASY3317	Intron 1	689 to 759	994 to 1064	Chr11:70079295-70079365	3	157	0.877	700
	Ta = 56	ANO1	TMEM16A	55107	ASY3309	5'-Upstream	-840 to -771	-535 to -466	Chr11:70077767-70077836	6	123	0.832	2200
		ANO1	TMEM16A	55107	ASY3381	Intron 12	71493 to 71528	71798 to 71833	Chr11:70150099-70150134	4	117	0.874	400
		ANO1	TMEM16A	55107	ASY2500	Intron 15	77571 to 77621	77876 to 77926	Chr11:70156177-70156227	5	114	0.857	500
		ANO1	TMEM16A	55107	ADS9064	Intron 2	22409 to 22475	22714 to 22780	Chr11:70101015-70101081	4	113	0.692	2000
		ANO1	TMEM16A	55107	ASY3375	Intron 2	11279 to 11331	11584 to 11636	Chr11:70089885-70089937	4	104	0.846	400
		ANO1	TMEM16A	55107	ASY3374	Intron 2	10351 to 10384	10656 to 10689	Chr11:70088957-70088990	4	97	0.831	1600
		ANO1	TMEM16A	55107	ASY3313	5'-UTR	-300 to -254	6 to 52	Chr11:70078307-70078353	8	95	0.896	600
	N162P3	ANO1	TMEM16A	55107	ASY3380	Intron 11	62559 to 62753	62864 to 63058	Chr11:70141165-70141359	11	243	0.786	100
	5-plex	ANO1	TMEM16A	55107	ASY3390	5'-Upstream	-1906 to -1817	-1601 to -1512	Chr11:70076701-70076790	3	199	0.89	100
	Ta = 59	ANO1	TMEM16A	55107	ASY3382	Intron 16	79636 to 79771	79941 to 80076	Chr11:70158242-70158377	7	192	0.709	500
		ANO1	TMEM16A	55107	ASY3376	Intron 2	12418 to 12548	12723 to 12853	Chr11:70091024-70091154	6	191	0.81	1100
		ANO1	TMEM16A	55107	ASY3377	Intron 3	25230 to 25290	25535 to 25595	Chr11:70103836-70103896	5	109	0.637	300
	3 PCRs	1 gene			19 assays					97 CpGs	2,722 bp

NGS158 - Human ST3GAL4 (ST3 beta-galactoside alpha-2,3-sialyltransferase 4)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N158P1	ST3GAL4	STZ	6484	ASY3333	Intron 1	-13841 to -13782	36630 to 36689	Chr11:126392315-126392374	2	145	0.893	100
	4-plex	ST3GAL4	STZ	6484	ASY3328	Intron 1	-49904 to -49872	567 to 599	Chr11:126356252-126356284	4	92	0.904	1400
	Ta = 62	ST3GAL4	STZ	6484	ASY3334	Intron 1	-520	49951	Chr11:126405636	1	90	0.911	200
		ST3GAL4	STZ	6484	ASY3326	5'-Upstream	-51078 to -51066	-608 to -596	Chr11:126355078-126355090	2	69	0.835	300
	N158P2	ST3GAL4	STZ	6484	ASY3331	Intron 1	-39417 to -39288	11054 to 11183	Chr11:126366739-126366868	6	192	0.86	500
	5-plex	ST3GAL4	STZ	6484	ASY3330	Intron 1	-49285 to -49254	1186 to 1217	Chr11:126356871-126356902	3	130	0.717	100
	Ta = 59	ST3GAL4	STZ	6484	ASY3338	Intron 9	7022 to 7067	57492 to 57537	Chr11:126413177-126413222	3	120	0.909	200
		ST3GAL4	STZ	6484	ASY3336	Intron 1, 5'-UTR	-87 to -45	50384 to 50426	Chr11:126406069-126406111	3	112	0.862	1300
		ST3GAL4	STZ	6484	ASY3337	Intron 9	6268 to 6310	56738 to 56780	Chr11:126412423-126412465	3	110	0.917	1300
	N158P3	ST3GAL4	STZ	6484	ASY3325	5'-Upstream	-51393 to -51355	-923 to -885	Chr11:126354763-126354801	2	195	0.887	100
	3-plex	ST3GAL4	STZ	6484	ASY3339	Exon 11	8072 to 8131	58542 to 58601	Chr11:126414227-126414286	5	149	0.907	600
	Ta = 53	ST3GAL4	STZ	6484	ASY3327	5'-Upstream	-50846 to -50754	-376 to -284	Chr11:126355310-126355402	7	143	0.854	200
	3 PCRs	1 gene			12 assays					41 CpGs	1,547 bp

NGS153 - Human FUT7 (fucosyltransferase 7)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N153P1	FUT7	FucT-VII	2529	ASY3096	5'-Upstream	-2717 to -2665	-2620 to -2568	Chr9:137034708-137034656	5	121	0.426	500
	8-plex	FUT7	FucT-VII	2529	ASY3034	5'-Upstream	-1083 to -1051	-986 to -954	Chr9:137033074-137033042	2	83	0.318	300
	Ta = 59	FUT7	FucT-VII	2529	ASY3103	5'-Upstream	-518 to -468	-421 to -371	Chr9:137032509-137032459	3	95	0.341	300
		FUT7	FucT-VII	2529	ASY3098	Intron 1	30	127	Chr9:137031962	1	87	0.385	1000
		FUT7	FucT-VII	2529	ASY3067	Exon 2	637 to 716	734 to 813	Chr9:137031355-137031276	7	137	0.461	50
		FUT7	FucT-VII	2529	ASY3101	3'-UTR	1287 to 1379	1384 to 1476	Chr9:137030705-137030613	7	153	0.373	300
		FUT7	FucT-VII	2529	ASY3069	3'-UTR	1471 to 1509	1568 to 1606	Chr9:137030521-137030483	4	100	0.444	200
		FUT7	FucT-VII	2529	ASY3088	3'-Downstream	3331 to 3374	3428 to 3471	Chr9:137028661-137028618	8	121	0.509	200
	N153P2	FUT7	FucT-VII	2529	ASY3029	5'-Upstream	-3685 to -3672	-3588 to -3575	Chr9:137035676-137035663	2	75	0.333	1200
	8-plex	FUT7	FucT-VII	2529	ASY3030	5'-Upstream	-3453 to -3406	-3356 to -3309	Chr9:137035444-137035397	5	143	0.389	100
	Ta = 59	FUT7	FucT-VII	2529	ASY3094	5'-Upstream	-3183 to -3080	-3086 to -2983	Chr9:137035174-137035071	5	160	0.284	100
		FUT7	FucT-VII	2529	ASY3095	5'-Upstream	-2795 to -2785	-2698 to -2688	Chr9:137034786-137034776	2	71	0.296	600
		FUT7	FucT-VII	2529	ASY3068	Exon 2	1028 to 1066	1125 to 1163	Chr9:137030964-137030926	5	100	0.41	1400
		FUT7	FucT-VII	2529	ASY3071	3'-Downstream	2349 to 2396	2446 to 2493	Chr9:137029643-137029596	4	123	0.421	700
		FUT7	FucT-VII	2529	ASY3072	3'-Downstream	2876 to 2961	2973 to 3058	Chr9:137029116-137029031	5	138	0.455	100
		FUT7	FucT-VII	2529	ASY3093	3'-Downstream	5914 to 5957	6011 to 6054	Chr9:137026078-137026035	4	112	0.457	500
	N153P3	FUT7	FucT-VII	2529	ASY3027	5'-Upstream	-4025 to -4019	-3928 to -3922	Chr9:137036016-137036010	2	110	0.297	500
	7-plex	FUT7	FucT-VII	2529	ASY3031	5'-Upstream	-3335 to -3271	-3238 to -3174	Chr9:137035326-137035262	4	126	0.241	300
	Ta = 59	FUT7	FucT-VII	2529	ASY3097	5'-Upstream	-2602 to -2509	-2505 to -2412	Chr9:137034593-137034500	4	152	0.212	200
		FUT7	FucT-VII	2529	ASY3035	5'-Upstream	-828 to -739	-731 to -642	Chr9:137032819-137032730	5	153	0.256	300
		FUT7	FucT-VII	2529	ASY3065	5'-Upstream, 5'-UTR	-104 to -36	-7 to 62	Chr9:137032095-137032027	5	134	0.292	100
		FUT7	FucT-VII	2529	ASY3090	3'-Downstream	4630 to 4714	4727 to 4811	Chr9:137027362-137027278	3	145	0.298	100
		FUT7	FucT-VII	2529	ASY3091	3'-Downstream	4909 to 4938	5006 to 5035	Chr9:137027083-137027054	3	130	0.324	100
	N153P4	FUT7	FucT-VII	2529	ASY3036	5'-Upstream	-298 to -225	-201 to -128	Chr9:137032289-137032216	2	138	0.577	300
	3-plex	FUT7	FucT-VII	2529	ASY3089	3'-Downstream	4430 to 4472	4527 to 4569	Chr9:137027562-137027520	3	105	0.234	50
	Ta = 59	FUT7	FucT-VII	2529	ASY3092	3'-Downstream	5662	5759	Chr9:137026330	1	115	0.529	400
	4 PCRs	1 gene			26 assays					101 CpGs	3,127 bp

NGS151 - Human CEBPA (CCAAT enhancer binding protein alpha)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N151P1	CEBPA	C/EBP-α	1050	ASY2946	5'-Upstream	-834 to -707	-714 to -587	Chr19:33303248-33303121	20	196	0.88	1200
	7-plex	CEBPA	C/EBP-α	1050	ASY2949	Exon 1, 3'-UTR	1071 to 1193	1191 to 1313	Chr19:33301344-33301222	15	175	0.989	100
	Ta = 56	CEBPA	C/EBP-α	1050	ASY2950	3'-UTR	1950 to 2021	2070 to 2141	Chr19:33300465-33300394	4	171	0.785	400
		CEBPA	C/EBP-α	1050	ASY2951	5'-Upstream	-3603 to -3547	-3483 to -3427	Chr19:33306017-33305961	2	170	0.995	1200
		CEBPA	C/EBP-α	1050	ASY2944	5'-Upstream, 5'-UTR	-136 to -21	-16 to 100	Chr19:33302550-33302435	23	166	0.907	700
		CEBPA	C/EBP-α	1050	ASY2942	5'-Upstream	-1371 to -1264	-1251 to -1144	Chr19:33303785-33303678	13	156	0.884	1000
		CEBPA	C/EBP-α	1050	ASY2940	5'-Upstream	-1933 to -1891	-1813 to -1771	Chr19:33304347-33304305	4	127	0.87	8000
	N151P2	CEBPA	C/EBP-α	1050	ASY2947	Exon 1	68 to 256	188 to 376	Chr19:33302347-33302159	28	243	0.863	100
	5-plex	CEBPA	C/EBP-α	1050	ASY2952	5'-Upstream	-3291 to -3189	-3171 to -3069	Chr19:33305705-33305603	4	226	0.992	100
	Ta = 59	CEBPA	C/EBP-α	1050	ASY2948	Exon 1	521 to 639	641 to 759	Chr19:33301894-33301776	20	172	0.948	300
		CEBPA	C/EBP-α	1050	ASY2945	5'-Upstream	-668 to -567	-548 to -447	Chr19:33303082-33302981	14	159	0.92	500
		CEBPA	C/EBP-α	1050	ASY2941	5'-Upstream	-1521 to -1427	-1401 to -1307	Chr19:33303935-33303841	12	146	0.946	1500
	2 PCRs	1 gene			12 assays					159 CpGs	2,107 bp

NGS149 - Human MAD1L1 (mitotic arrest deficient 1 like 1)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N149P1	MAD1L1	MAD1	8379	ASY2875	5'-Upstream	-4681 to -4571	-1571 to -1461	Chr7:2234814-2234704	4	186	0.8	500
	7-plex	MAD1L1	MAD1	8379	ASY2863	Intron 4, Exon 5	7327 to 7442	10437 to 10552	Chr7:2222807-2222692	12	175	0.821	3500
	Ta = 62	MAD1L1	MAD1	8379	ASY2882	Intron 18	410716 to 410820	413826 to 413930	Chr7:1819418-1819314	9	166	0.815	3000
		MAD1L1	MAD1	8379	ASY2868	Intron 11	118435 to 118509	121545 to 1221619	Chr7:2111699-2111625	5	150	0.709	1000
		MAD1L1	MAD1	8379	ASY2870	Intron 12	183124 to 183213	186234 to 186323	Chr7:2047010-2046921	6	144	0.899	4000
		MAD1L1	MAD1	8379	ASY2879	Intron 11	82533 to 82600	85643 to 85710	Chr7:2147601-2147534	5	132	0.78	600
		MAD1L1	MAD1	8379	ASY2883	Intron 18	411020 to 411088	414130 to 414198	Chr7:1819114-1819046	6	121	0.931	5000
	N149P2	MAD1L1	MAD1	8379	ASY2871	Intron 16	288867 to 288979	291977 to 292089	Chr7:1941267-1941155	11	247	0.515	100
	6-plex	MAD1L1	MAD1	8379	ASY2867	Intron 1	-2342 to -2217	769 to 894	Chr7:2232475-2232350	10	173	0.679	1500
	Ta = 59	MAD1L1	MAD1	8379	ADS4001	Intron 17	307865 to 307951	310975 to 311061	Chr7:1922269-1922183	11	149	0.779	10000
		MAD1L1	MAD1	8379	ASY2869	Intron 14	229504 to 229579	232614 to 232689	Chr7:2000630-2000555	7	144	0.54	7000
		MAD1L1	MAD1	8379	ASY2865	5'-Upstream	-3346 to -3316	-236 to -206	Chr7:2233479-2233449	2	118	0.763	5000
		MAD1L1	MAD1	8379	ASY2873	Exon 4, Intron 4	4721 to 4748	7831 to 7858	Chr7:2225413-2225386	4	93	0.74	3500
	N149P3	MAD1L1	MAD1	8379	ASY2884	Intron 12	169521 to 169538	172631 to 172648	Chr7:2060613-2060596	3	210	0.664	50
	5-plex	MAD1L1	MAD1	8379	ASY2880	Intron 11	126261 to 126348	129371 to 129458	Chr7:2103873-2103786	4	166	-	100
	Ta = 56	MAD1L1	MAD1	8379	ASY1554	Intron 14	242401 to 242485	245511 to 245595	Chr7:1987733-1987649	13	152	0.655	100
		MAD1L1	MAD1	8379	ASY2872	Intron 16	289638 to 289720	292748 to 292830	Chr7:1940496-1940414	5	148	0.697	100
		MAD1L1	MAD1	8379	ASY2881	Intron 12	183428 to 183508	186538 to 186618	Chr7:2046706-2046626	7	141	0.89	3000
		MAD1L1	MAD1	8379	ASY2922	Exon 5	7476 to 7524	10586 to 10634	Chr7:2222658-2222610	2	104	0.856	10000
	3 PCRs	1 gene			19 assays					126 CpGs	2,919 bp

NGS148 - Human CDKN1A (cyclin dependent kinase inhibitor 1A)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N148P1	CDKN1A	p21	1026	ASY2843	5'-Upstream	-5653 to -5524	-209 to -80	Chr6:36678449-36678578	11	216	0.883	50
	6-plex	CDKN1A	p21	1026	ASY2848	Intron 1	-582 to -527	4863 to 4918	Chr6:36683520-36683575	3	146	0.926	950
	Ta = 59	CDKN1A	p21	1026	ASY2839	5'-Upstream	-6544	-1100	Chr6:36677558	1	142	0.954	400
		CDKN1A	p21	1026	ASY2844	5'-UTR	-5371 to -5320	74 to 125	Chr6:36678731-36678782	7	101	0.939	2300
		CDKN1A	p21	1026	ASY2846	5'-Upstream	-6928 to -6882	-1484 to -1438	Chr6:36677174-36677220	2	93	0.943	1500
		CDKN1A	p21	1026	ASY2838	5'-Upstream	-6791 to -6779	-1347 to -1335	Chr6:36677311-36677323	2	89	0.925	1200
	N148P2	CDKN1A	p21	1026	ADS1253-1	5'-Upstream, 5'-UTR	-5489 to -5406	-45 to 39	Chr6:36678613-36678696	15	152	0.931	120
	4-plex	CDKN1A	p21	1026	ASY2842	5'-Upstream	-5846 to -5752	-402 to -308	Chr6:36678256-36678350	2	149	0.918	1800
	Ta = 59	CDKN1A	p21	1026	ASY2837	5'-Upstream	-6977	-1533	Chr6:36677125	1	117	0.942	1800
		H2BC1	TSH2B	255626	ASY2850	5'-UTR, Exon 1	-30 to 5	103 to 137	Chr6:25726879-25726913	3	88	0.96	2800
	N148P3	CDKN1A	p21	1026	ASY2845	Intron 1	-5271 to -5057	174 to 388	Chr6:36678831-36679045	31	290	0.924	160
	5-plex	CDKN1A	p21	1026	ASY2840	5'-Upstream	-6379 to -6231	-935 to -787	Chr6:36677723-36677871	3	226	0.937	100
	Ta = 59	H2BC1	TSH2B	255626	ASY2849	5'-Upstream, 5'-UTR	-273 to -118	-141 to 15	Chr6:25726636-25726791	11	222	0.939	1600
		CDKN1A	p21	1026	ASY2841	5'-Upstream	-6067 to -5993	-623 to -549	Chr6:36678035-36678109	2	163	0.923	150
		CDKN1A	p21	1026	ASY2847	Intron 1	-4871 to -4769	574 to 676	Chr6:36679231-36679333	8	150	0.976	350
	3 PCRs	2 genes			15 assays					102 CpGs	2,344 bp

NGS144/139/116 - Human Mitochondrial Fusion	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N144P1	POLRMT	MTRPOL	5442	ASY2704	Exon 3	3615 to 3728	3640 to 3753	Chr19:629898-629785	8	171	0.885	1200
	10-plex	RAF1	CRAF	5894	ASY2732	5'-Upstream	-45667 to -45553	-271 to -157	Chr3:12664388-12664274	12	164	0.857	300
	Ta = 61	MTIF3	IF3MT	219402	ASY2710	Intron 2	-8979 to -8876	1138 to 1241	Chr13:27449427-27449324	4	158	0.93	200
		MFN2	CMT2A	9927	ASY2682	5'-Upstream	-9514 to -9418	-657 to -561	Chr1:11979655-11979751	6	155	0.882	500
		FIS1	TTC11	51024	ASY2722	5'-UTR, Intron 1	-16 to 59	62 to 136	Chr7:101245020-101244946	5	139	0.884	1800
		PDPK1	PDK1	5170	ASY2740	Intron 11	55588 to 55640	55680 to 55732	Chr16:2593700-2593752	3	132	0.944	200
		FIS1	TTC11	51024	ASY2721	5'-Upstream	-203 to -147	-126 to -70	Chr7:101245207-101245151	10	122	0.872	800
		TFAM	MTTF1	7019	ASY2688	5'-UTR, Exon 1	-39 to 27	100 to 165	Chr10:58385509-58385574	7	122	0.989	400
		RAF1	CRAF	5894	ASY2734	Intron 1	-44469 to -44413	928 to 984	Chr3:12663190-12663134	8	106	0.97	1000
		OPA1	MGM1	4976	ASY2680	3'-Downstream	108532 to 108552	108724 to 108744	Chr3:193701909-193701929	4	95	0.998	250
	N144P2	PDPK1	PDK1	5170	ASY2738	Intron 1	333 to 442	425 to 534	Chr16:2538445-2538554	9	163	0.884	250
	8-plex	PRKN	PARK2	5071	ASY2749	Intron 1	963 to 967	1061 to 1065	Chr6:162726706-162726702	2	157	0.996	1000
	Ta = 61	TFB2M	HKP1	64216	ASY2694	5'-Upstream, 5'-UTR	-146 to -62	-23 to 62	Chr1:246566284-246566200	8	152	0.975	200
		APC	DP2.5	324	ASY2745	5'-UTR	-166 to -83	31 to 114	Chr5:112707552-112707635	10	145	0.989	300
		DNM1L	DRP1	10059	ASY2713	Exon 1	8 to 87	71 to 150	Chr12:32679371-32679450	7	135	0.99633769630805	250
		FIS1	TTC11	51024	ASY2723	Intron 1	535 to 604	612 to 681	Chr7:101244470-101244401	8	121	0.904	3500
		PTEN	MMAC1	5728	ASY2754	Intron 1	3601 to 3628	4446 to 4473	Chr10:87868070-87868097	2	110	0.98	100
		TFAM	MTTF1	7019	ASY2689	Exon 1, Intron 1	69 to 109	207 to 247	Chr10:58385616-58385656	4	106	0.982	250
	N144P3	TFAM	MTTF1	7019	ASY2687	5'-Upstream	-454 to -333	-316 to -195	Chr10:58385094-58385215	15	173	0.98	600
	7-plex	RAF1	CRAF	5894	ASY2731	5'-Upstream	-45826 to -45739	-430 to -343	Chr3:12664547-12664460	7	148	0.939	9000
	Ta = 59	PDPK1	PDK1	5170	ASY2739	Intron 1	1607 to 1692	1699 to 1784	Chr16:2539719-2539804	5	142	0.908	2200
		POLRMT	MTRPOL	5442	ASY2705	Exon 10	11878 to 11951	11903 to 11976	Chr19:621635-621562	11	132	0.896	2000
		MFN2	CMT2A	9927	ASY2685	Intron 9, Exon 10	12547 to 12611	21404 to 21468	Chr1:12001715-12001779	3	124	0.93	100
		NAMPT	PBEF1	10135	ASY2728	5'-Upstream	-693 to -634	-594 to -535	Chr7:106285577-106285518	5	106	0.96683762238313	10000
		PRKN	PARK2	5071	ASY2748	5'-Upstream	-657 to -621	-559 to -523	Chr6:162728325-162728289	3	92	0.962	100
	N144P4	NFE2L2	NRF2	4780	ADS3188	5'-Upstream	-844 to -736	-693 to -585	Chr2:177265420-177265312	8	183	0.924	100
	8-plex	MTERF4	MTERFD2	130916	ASY2718	Exon 3	4894 to 4996	4916 to 5018	Chr2:241097380-241097278	4	168	0.977	150
	Ta = 56	APC	DP2.5	324	ASY2743	Intron 1	30193 to 30287	30389 to 30483	Chr5:112737910-112738004	5	161	0.898	200
		RAF1	CRAF	5894	ASY2733	Intron 1	-45077 to -44978	320 to 419	Chr3:12663798-12663699	14	152	0.96	1800
		GABPA	E4TF1A	2551	ASY2700	Intron 1	-5238 to -5174	1792 to 1856	Chr21:25736361-25736425	5	148	0.937	100
		FIS1	TTC11	51024	ASY2724	Exon 2, Intron 2	974 to 1058	1051 to 1135	Chr7:101244031-101243947	7	142	0.844	100
		MTIF3	IF3MT	219402	ASY2707	5'-Upstream	-10337 to -10288	-221 to -172	Chr13:27450785-27450736	5	110	0.863	400
		OPA1	MGM1	4976	ASY2679	Intron 28	94811 to 94833	95003 to 95025	Chr3:193688188-193688210	3	103	0.987	2500
	N144P5	APC	DP2.5	324	ASY516	Intron 1	29984 to 30130	30180 to 30326	Chr5:112737701-112737847	16	194	0.989	1200
	10-plex	APC	DP2.5	324	ASY2744	Intron 1	30544 to 30676	30740 to 30872	Chr5:112738261-112738393	4	191	0.984	150
	Ta = 56	PRKN	PARK2	5071	ADS5123	5'-Upstream	-379 to -263	-281 to -165	Chr6:162728047-162727931	10	174	0.99	900
		OPA1	MGM1	4976	ASY2681	5'-UTR, Exon 1	-76 to 21	117 to 213	Chr3:193593302-193593398	16	152	0.989	200
		PDPK1	PDK1	5170	ASY2737	5'-UTR	-79 to -8	14 to 85	Chr16:2538034-2538105	15	132	0.722	1200
		MFN2	CMT2A	9927	ASY2684	Intron 2	-5633 to -5571	3225 to 3287	Chr1:11983536-11983598	2	123	0.851	2000
		DNM1L	DRP1	10059	ASY2711	5'-Upstream	-929 to -862	-866 to -799	Chr12:32678435-32678502	4	122	0.886	150
		MTIF3	IF3MT	219402	ASY2708	Intron 1	-9952 to -9888	165 to 229	Chr13:27450400-27450336	9	115	0.871	750
		TFAM	MTTF1	7019	ASY2690	Intron 4	3699	3837	Chr10:58389246	1	95	0.99438251521103	400
		NFE2L2	NRF2	4780	ADS8779	5'-Upstream	-1493	-1342	Chr2:177266069	1	85	0.92865032033087	250
	N144P6	PTEN	MMAC1	5728	ADS3543	5'-Upstream	-2487 to -2405	-1642 to -1560	Chr10:87861983-87862065	8	172	0.986	2000
	9-plex	MFN2	CMT2A	9927	ASY2683	5'-Upstream	-9112 to -9004	-255 to -147	Chr1:11980057-11980165	12	163	0.757	300
	Ta = 56	MTERF4	MTERFD2	130916	ASY2720	3'-UTR	13751 to 13852	13773 to 13874	Chr2:241088523-241088422	6	154	0.979	400
		OPA1	MGM1	4976	ASY2678	Intron 1	12627	12819	Chr3:193606004	1	138	0.99370792499981	1500
		GABPA	E4TF1A	2551	ADS3958re	5'-Upstream	-7266 to -7179	-237 to -150	Chr21:25734333-25734420	4	138	0.944	100
		MTERF4	MTERFD2	130916	ASY2719	Intron 5	8262 to 8316	8284 to 8338	Chr2:241094012-241093958	2	119	0.894	1500
		APC	DP2.5	324	ASY2746	5'-Upstream	-335 to -278	-139 to -82	Chr5:112707383-112707440	7	108	0.99	3000
		TFB2M	HKP1	64216	ASY2695	Exon 1	217 to 254	340 to 377	Chr1:246565922-246565885	5	102	0.94	3200
		RAF1	CRAF	5894	ASY2735	Intron 1	-41557	3840	Chr3:12660278	1	90	0.99714316865821	150
	N139P1	PPARGC1A	PGC1A	10891	ASY1844	Intron 1	3797 to 3927	3887 to 4017	Chr4:23886161-23886031	3	205	0.996	3500
	6-plex	DOC2B	DOC2BL	8447	ASY1835	Intron 1	1475 to 1593	1646 to 1764	Chr17:180005-179887	4	195	0.502	1200
	Ta = 62	MFN1	HFZO1	55669	ASY1850	Intron 1	-894 to -761	250 to 383	Chr3:179347958-179348091	18	182	0.98	1000
		LIPT1	LIPT1D	51601	ASY2567	Intron 2	-2186 to -2137	4818 to 4867	Chr2:99159772-99159821	3	167	0.988	500
		LIPT1	LIPT1D	51601	ASY2563	5'-Upstream	-7311 to -7227	-308 to -224	Chr2:99154647-99154731	8	151	0.982	2000
		DOC2B	DOC2BL	8447	ASY2578	Intron 3	17831 to 17851	18002 to 18022	Chr17:163649-163629	4	113	0.557	500
	N139P2	DOC2B	DOC2BL	8447	ASY2572	Intron 1	2315 to 2421	2486 to 2592	Chr17:179165-179059	5	181	0.542	200
	10-plex	STOML2	SLP2	30968	ASY2588	Exon 7	1828 to 1923	1865 to 1960	Chr9:35101267-35101172	6	171	0.923	400
	Ta = 59	PPARGC1A	PGC1A	10891	ASY1840	5'-Upstream	-961 to -903	-871 to -813	Chr4:23890918-23890860	3	149	0.989	3000
		STOML2	SLP2	30968	ASY2587	Exon 6	1601 to 1643	1638 to 1680	Chr9:35101494-35101452	3	140	0.932	3000
		PPARGC1A	PGC1A	10891	ASY1843	Intron 1	1326 to 1374	1416 to 1464	Chr4:23888632-23888584	2	134	0.972	150
		DOC2B	DOC2BL	8447	ASY2568	5'-Upstream	-1234 to -1176	-1063 to -1005	Chr17:182713-182655	3	131	0.66	400
		STOML2	SLP2	30968	ASY2586	Exon 5	1316 to 1383	1353 to 1420	Chr9:35101779-35101712	3	128	0.905	900
		LIPT1	LIPT1D	51601	ASY2562	5'-Upstream	-7410 to -7356	-407 to -353	Chr2:99154548-99154602	3	122	0.995	150
		STOML2	SLP2	30968	ASY2580	5'-Upstream	-543 to -500	-506 to -463	Chr9:35103637-35103594	3	103	0.873	150
		DOC2B	DOC2BL	8447	ASY2571	5'-Upstream	-652 to -650	-481 to -479	Chr17:182131-182129	2	102	0.72	1000
	N139P3	DOC2B	DOC2BL	8447	ASY2569	5'-Upstream	-1130 to -997	-959 to -826	Chr17:182609-182476	5	204	0.672	100
	11-plex	PPARGC1A	PGC1A	10891	ASY1847	Intron 12	92946 to 93055	93036 to 93145	Chr4:23797012-23796903	3	195	0.991	700
	Ta = 56	STOML2	SLP2	30968	ASY2583	5'-Upstream, 5'-UTR, Exon 1	-127 to 19	-90 to 56	Chr9:35103221-35103076	20	190	0.988	1500
		PPARGC1A	PGC1A	10891	ADS2164	5'-UTR, Intron 1	-17 to 94	74 to 184	Chr4:23889974-23889864	6	174	0.998	400
		LIPT1	LIPT1D	51601	ASY2561	5'-Upstream	-7527 to -7464	-524 to -461	Chr2:99154431-99154494	3	168	0.975	200
		LIPT1	LIPT1D	51601	ASY2566	Intron 1	-6543 to -6437	461 to 567	Chr2:99155415-99155521	5	168	0.96	8000
		DOC2B	DOC2BL	8447	ASY2573	Intron 1	2561 to 2635	2732 to 2806	Chr17:178919-178845	2	150	0.617	400
		LIPT1	LIPT1D	51601	ASY1833	Exon 3	507 to 560	7510 to 7563	Chr2:99162464-99162517	3	138	0.993	3000
		STOML2	SLP2	30968	ASY2585	Intron 3, Exon 4	1110 to 1171	1147 to 1208	Chr9:35101985-35101924	3	122	0.922	1000
		STOML2	SLP2	30968	ASY2582	Intron 2	461 to 514	498 to 551	Chr9:35102634-35102581	4	121	0.921	1500
		LIPT1	LIPT1D	51601	ASY2564	5'-Upstream	-7190 to -7140	-187 to -137	Chr2:99154768-99154818	5	119	0.989	1500
	N139P4	STOML2	SLP2	30968	ASY2579	5'-Upstream	-455 to -316	-418 to -279	Chr9:35103549-35103410	8	186	0.871	6000
	8-plex	DOC2B	DOC2BL	8447	ASY2570	5'-Upstream	-950 to -854	-779 to -683	Chr17:182429-182333	4	158	0.616	1300
	Ta = 59	MFN1	HFZO1	55669	ASY1849	5'-Upstream	-1293 to -1196	-150 to -53	Chr3:179347559-179347656	5	158	0.947	2500
		LIPT1	LIPT1D	51601	ASY2565	Intron 1	-6680 to -6605	324 to 399	Chr2:99155278-99155353	6	141	0.995	4500
		STOML2	SLP2	30968	ASY2584	Exon 3	931 to 999	968 to 1036	Chr9:35102164-35102096	5	130	0.934	5000
		PPARGC1A	PGC1A	10891	ASY1838	5'-Upstream	-1042	-952	Chr4:23890999	1	106	0.992	100
		DOC2B	DOC2BL	8447	ASY1834	5'-Upstream	-2358 to -2338	-2187 to -2167	Chr17:183837-183817	2	103	0.501	400
		PPARGC1A	PGC1A	10891	ASY1837	5'-Upstream	-1310	-1220	Chr4:23891267	1	97	0.992	500
	N139P5	MFN1	HFZO1	55669	ASY1852	Intron 9, Exon 10	26326 to 26404	27469 to 27547	Chr3:179375177-179375255	5	208	0.962	200
	6-plex	MFN1	HFZO1	55669	ASY1851	Intron 10	27364 to 27437	28507 to 28580	Chr3:179376215-179376288	3	189	0.937	4500
	Ta = 56	STOML2	SLP2	30968	ADS5520	5'-Upstream	-293 to -150	-256 to -113	Chr9:35103387-35103244	12	186	0.872	1000
		DOC2B	DOC2BL	8447	ASY2577	Intron 2	16501 to 16562	16672 to 16733	Chr17:164979-164918	4	172	0.65	700
		PPARGC1A	PGC1A	10891	ASY1841	Intron 1	604 to 699	694 to 789	Chr4:23889354-23889259	8	165	0.991	300
		PPARGC1A	PGC1A	10891	ASY1842	Intron 1	879 to 965	969 to 1055	Chr4:23889079-23888993	6	141	0.989	200
	N139P6	PPARGC1A	PGC1A	10891	ASY1845	Intron 2	14068 to 14292	14158 to 14382	Chr4:23875890-23875666	4	275	0.984	300
	3-plex	PPARGC1A	PGC1A	10891	ASY1848	3'-UTR	94956 to 95127	95046 to 95217	Chr4:23795002-23794831	5	237	0.982	100
	Ta = 62	STOML2	SLP2	30968	ASY2581	Intron 1	53 to 113	90 to 150	Chr9:35103042-35102982	3	118	0.947	2500
	N116P1	UQCRC2	QCR2	7385	ADS9486	5'-UTR, Intron 1	-88 to 48	677 to 812	Chr16:21953336-21953471	9	189	0.991	100
	9-plex	PCDH9	n/a	5101	ADS9490	5'-UTR, Intron 1	-1340 to -1251	666 to 755	Chr13:67229780-67229691	4	150	0.968	500
	Ta = 59	PCCB	n/a	5096	ADS9481	Intron 1	670 to 769	740 to 839	Chr3:136251045-136251144	7	149	0.996	400
		PCDHA12	PCDHα12	56137	ADS9492	Intron 1	7177 to 7270	7177 to 7270	Chr5:140882649-140882742	7	142	0.954	100
		RIMS3	NIM3	9783	ADS9493	5'-Upstream	-27205	-3473	Chr1:40669130	1	142	0.982	1000
		PCDHA12	PCDHα12	56137	ADS9491	Exon 1	327 to 392	327 to 392	Chr5:140875799-140875864	6	124	0.836	250
		ASTN1	ASTN	460	ADS9470	Intron 16	266667 to 266732	266681 to 266746	Chr1:176898010-176897945	6	120	0.994	800
		FBXO15	FBX15	201456	ADS9475	5'-Upstream	-296 to -227	-216 to -147	Chr18:74148081-74148012	7	119	0.91	800
		MMUT	MCM	4594	ADS9479	5'-Upstream	-3889 to -3833	-164 to -108	Chr6:49463355-49463299	5	100	0.988	300
	N116P2	TTC25	n/a	83538	ADS9468	5'-Upstream	-263 to -163	-195 to -95	Chr17:41930461-41930561	10	160	0.983	150
	3-plex	BBS4	n/a	585	ADS9487	Intron 1	301 to 377	342 to 418	Chr15:72686528-72686604	3	137	0.99	100
	Ta = 55	CDH8	n/a	1006	ADS9488	Intron 1	-13197 to -13128	2236 to 2305	Chr16:62034600-62034531	7	113	0.858	100
	N116P3	SIN3A	WITKOS	25942	ADS9494	5'-Upstream	-21838 to -21677	-509 to -348	Chr15:75452213-75452052	11	220	0.934	100
	6-plex	SRSF11	p54	9295	ADS9484	Intron 1	-15681 to -15558	233 to 356	Chr1:70205956-70206079	9	215	0.998	700
	Ta = 56	MTR	MS	4548	ADS9478	Intron 1	81 to 188	475 to 582	Chr1:236795784-236795891	12	151	0.979	100
		CYB5B	OMB5	80777	ADS9485	5'-Upstream	-233 to -133	-144 to -44	Chr16:69424451-69424551	7	151	0.997	400
		CUL3	PHA2E	8452	ADS9473	Intron 1	802 to 847	1141 to 1186	Chr2:224584208-224584163	4	108	0.846	300
		EHHADH	ECHD	1962	ADS9474	5'-Upstream	-279 to -271	-203 to -195	Chr3:185254301-185254293	2	89	0.993	200
	N116P4	CSNK2A1	CK2A1	1457	ADS9472	5'-Upstream, 5'-UTR	-35334 to -35182	-62 to 91	Chr20:543885-543733	15	208	0.788	400
	6-plex	PRMT1	HRMT1L2	3276	ADS9482	5'-Upstream, 5'-UTR	-178 to -61	-49 to 69	Chr19:49677103-49677220	11	178	0.815	400
	Ta = 56	MAGT1	IAP	84061	ADS9471	5'-Upstream, 5'-UTR	-144 to -21	-57 to 67	ChrX:77895650-77895527	11	174	0.879	1000
		JMJD1C	TRIP8	221037	ADS9489	Intron 2	196442 to 196493	196661 to 196712	Chr10:63269221-63269170	2	117	0.956	400
		MSANTD4	KIAA1826	84437	ADS9467	5'-Upstream	-11405 to -11350	-95 to -40	Chr11:106022322-106022267	4	103	0.995	250
		AHI1	JBTS3	54806	ADS9469	Intron 1	-5165 to -5128	344 to 381	Chr6:135497402-135497365	4	91	0.994	500
	16 PCRs	55 genes			120 assays					719 CpGs	17,447 bp

NGS142 - Human EPHA2 (ephrin type-A receptor 2)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N142P1	EPHA2	ECK	1969	ASY2652	Exon 9, Intron 9	22031 to 22115	22168 to 22252	Chr1:16133902-16133818	8	153	0.84533333333333	900
	8-plex	EPHA2	ECK	1969	ASY2612	5'-Upstream	-3559 to -3465	-3422 to -3328	Chr1:16159491-16159397	3	153	0.997	1400
	Ta = 59	EPHA2	ECK	1969	ASY2620	Intron 1	1534 to 1624	1671 to 1761	Chr1:16154399-16154309	4	141	0.86266666666667	700
		EPHA2	ECK	1969	ASY2616	5'-Upstream	-416 to -331	-279 to -194	Chr1:16156348-16156263	7	137	0.88666666666667	1200
		EPHA2	ECK	1969	ASY2636	Intron 3	11834 to 11887	11971 to 12024	Chr1:16144099-16144046	3	128	0.804	2300
		EPHA2	ECK	1969	ASY2641	Intron 3	16510	16647	Chr1:16139423	1	128	0.82974721264794	400
		EPHA2	ECK	1969	ASY2647	Intron 6	20460 to 20475	20597 to 20612	Chr1:16135473-16135458	2	116	0.91066666666667	1900
		EPHA2	ECK	1969	ASY2645	Exon 5	17896 to 17948	18033 to 18085	Chr1:16138037-16137985	6	106	0.89933333333333	1400
	N142P2	EPHA2	ECK	1969	ASY2656	Exon 16	26344 to 26404	26481 to 26541	Chr1:16129589-16129529	8	155	0.86433333333333	400
	6-plex	EPHA2	ECK	1969	ASY2643	Intron 3	16854 to 16931	16991 to 17068	Chr1:16139079-16139002	3	149	0.87733333333333	300
	Ta = 56	EPHA2	ECK	1969	ASY2637	Intron 3	11985 to 12021	12122 to 12158	Chr1:16143948-16143912	2	116	0.861	800
		EPHA2	ECK	1969	ASY2608	5'-Upstream	-6789 to -6759	-6652 to -6622	Chr1:16162721-16162691	3	100	0.994	200
		EPHA2	ECK	1969	ASY2663	3'-Downstream	33044 to 33069	33181 to 33206	Chr1:16122889-16122864	3	99	0.96633333333333	1200
		EPHA2	ECK	1969	ASY2650	Intron 8	21569 to 21607	21706 to 21744	Chr1:16134364-16134326	4	92	0.898	400
	N143P3	EPHA2	ECK	1969	ASY2639	Intron 3	12404	12541	Chr1:16143529	1	154	0.80187513687507	200
	9-plex	EPHA2	ECK	1969	ASY2624	Intron 1	4543 to 4587	4680 to 4724	Chr1:16151390-16151346	2	120	0.91633333333333	1300
	Ta = 56	EPHA2	ECK	1969	ASY2619	Intron 1	742 to 792	879 to 929	Chr1:16155191-16155141	4	110	0.795	700
		EPHA2	ECK	1969	ASY2618	Intron 1	435 to 490	572 to 627	Chr1:16155498-16155443	5	106	0.87266666666667	300
		EPHA2	ECK	1969	ASY2627	Exon 3	7287 to 7338	7424 to 7475	Chr1:16148646-16148595	5	102	0.842	1400
		EPHA2	ECK	1969	ASY2607	5'-Upstream	-6887 to -6840	-6750 to -6703	Chr1:16162819-16162772	3	98	0.985	6000
		EPHA2	ECK	1969	ASY2657	Intron 16	27386 to 27411	27523 to 27548	Chr1:16128547-16128522	2	96	0.90933333333333	2200
		EPHA2	ECK	1969	ASY2651	Intron 8	21892 to 21904	22029 to 22041	Chr1:16134041-16134029	2	95	0.91366666666667	1000
		EPHA2	ECK	1969	ASY2633	Intron 3	10571 to 10596	10708 to 10733	Chr1:16145362-16145337	4	85	0.823	400
	N142P4	EPHA2	ECK	1969	ASY2614	5'-Upstream	-1291 to -1220	-1154 to -1083	Chr1:16157223-16157152	5	144	0.91866666666667	700
	7-plex	EPHA2	ECK	1969	ASY2646	Exon 5	18001 to 18050	18138 to 18187	Chr1:16137932-16137883	5	123	0.857	400
	Ta = 53	EPHA2	ECK	1969	ASY2660	Intron 16	28315 to 28361	28452 to 28498	Chr1:16127618-16127572	3	117	0.882	400
		EPHA2	ECK	1969	ASY2644	Intron 3	17101 to 17149	17238 to 17286	Chr1:16138832-16138784	3	117	0.90266666666667	900
		EPHA2	ECK	1969	ASY2658	Intron 16	27594 to 27658	27731 to 27795	Chr1:16128339-16128275	4	116	0.83033333333333	500
		EPHA2	ECK	1969	ASY2623	Intron 1	3536 to 3565	3673 to 3702	Chr1:16152397-16152368	2	116	0.905	400
		EPHA2	ECK	1969	ASY2617	5'-Upstream	-198 to -180	-61 to -43	Chr1:16156130-16156112	4	75	0.94566666666667	1600
	4 PCRs	1 gene			30 assays					111 CpGs	3,547 bp

NGS140 - Human Metabolism	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N140P1	PPARGC1A	PGC1A	10891	ASY1845	Intron 2	14068 to 14292	14158 to 14382	Chr4:23875890-23875666	4	275	0.978	250
	6-plex	PPARGC1A	PGC1A	10891	ASY1844	Intron 1	3797 to 3927	3887 to 4017	Chr4:23886161-23886031	3	205	0.968	2000
	Ta = 62	INS	n/a	3630	ASY1823	5'-Upstream	-1680 to -1624	-1447 to -1391	Chr11:2162651-2162595	5	197	0.226	500
		PIK3R5	p101	23533	ASY1828	5'-Upstream	-54295 to -54233	-77 to -15	Chr17:8965789-8965727	9	102	0.956	600
		PPARGC1A	PGC1A	10891	ASY1846	Intron 7	67656	67746	Chr4:23822302	1	100	0.99048535544485	7500
		INS	n/a	3630	ASY3006	5'-UTR	-112 to -100	122 to 134	Chr11:2161083-2161071	2	62	0.396	4500
	N140P2	PPARGC1A	PGC1A	10891	ASY1847	Intron 12	92946 to 9305	93036 to 93145	Chr4:23797012-23796903	3	195	0.971	200
	6-plex	PPARA	NR1C1	5465	ASY1654R	5'-Upstream	-48806 to -48685	-2306 to -2185	Chr22:46149578-46149699	14	168	0.759	500
	Ta = 59	INS	n/a	3630	ADS3196	Intron 1	561 to 652	794 to 885	Chr11:2160411-2160320	7	154	0.312	150
		PPARGC1A	PGC1A	10891	ASY1840	5'-Upstream	-961 to -903	-871 to -813	Chr4:23890918-23890860	3	149	0.994	1800
		PIK3R5	p101	23533	ASY1829	Intron 1	-4755 to -4719	49464 to 49500	Chr17:8916249-8916213	3	112	0.961	1200
		INS	n/a	3630	ASY1822	5'-Upstream	-1765 to -1716	-1532 to -1483	Chr11:2162736-2162687	3	105	0.211	700
	N140P3	PPARGC1A	PGC1A	10891	ADS2164	5'-UTR, Intron 1	-17 to 94	74 to 184	Chr4:23889974-23889864	6	174	0.992	800
	5-plex	PPARGC1A	PGC1A	10891	ASY1841	Intron 1	604 to 699	694 to 789	Chr4:23889354-23889259	8	165	0.986	600
	Ta = 56	INS	n/a	3630	ADS1531	5'-Upstream	-373 to -307	-140 to -74	Chr11:2161344-2161278	3	138	0.292	1100
		PIK3R5	p101	23533	ASY1824	Exon 10	22875 to 22951	77093 to 77169	Chr17:8888620-8888544	8	131	0.74	5000
		PPARGC1A	PGC1A	10891	ASY1837	5'-Upstream	-1310	-1220	Chr4:23891267	1	97	0.9824197179801	2200
	N140P4	PIK3R5	p101	23533	ASY1825	Exon 10	22719 to 22825	76937 to 77043	Chr17:8888776-8888670	7	183	0.868	500
	5-plex	PPARA	NR1C1	5465	ASY1652	5'-Upstream	-48959 to -48854	-2459 to -2354	Chr22:46149425-46149530	7	175	0.781	1300
	Ta = 56	PIK3R5	p101	23533	ASY1827	5'-Upstream	-55565 to -55513	-1347 to -1295	Chr17:8967059-8967007	4	142	0.955	3400
		PPARGC1A	PGC1A	10891	ASY1842	Intron 1	879 to 965	969 to 1055	Chr4:23889079-23888993	6	141	0.987	750
		PPARGC1A	PGC1A	10891	ASY1838	5'-Upstream	-1042	-952	Chr4:23890999	1	106	0.99486635808169	1100
	N135P1	INSR	CD220	3643	ADS1572	Intron 2	48226 to 48411	48749 to 48934	Chr19:7245666-7245481	5	256	0.985	3700
	4-plex	WNT10B	WNT12	7480	ASY1662	5'-Upstream	-3171 to -3074	-1965 to -1868	Chr12:48973700-48973603	8	186	0.809	350
	Ta = 59	FZD8	FZ8	8325	ASY1657	5'-Upstream	-3173 to -3039	-2324 to -2190	Chr10:35644602-35644468	5	184	0.794	1200
		CAMK2B	CAMK2	816	ASY1663	Intron 1	1103 to 1164	1276 to 1337	Chr7:44324319-44324258	6	175	0.889	100
	N135P2	FZD8	FZ8	8325	ASY1658	5'-Upstream	-2965 to -2911	-2116 to -2062	Chr10:35644394-35644340	3	168	0.874	2800
	5-plex	WNT10B	WNT12	7480	ASY1661	5'-Upstream	-3272 to -3223	-2066 to -2017	Chr12:48973801-48973752	4	145	0.882	250
	Ta = 56	CAMK2B	CAMK2	816	ASY1656	Exon 1, Intron 1	25 to 95	198 to 268	Chr7:44325397-44325327	10	121	0.823	4000
		FZD8	FZ8	8325	ASY1659	5'-Upstream	-2755 to -2735	-1906 to -1886	Chr10:35644184-35644164	3	116	0.869	1600
		CAMK2B	CAMK2	816	ASY1653	5'-Upstream	-551 to -499	-378 to -326	Chr7:44325972-44325920	8	107	0.909	3800
	N135P3	PPARA	NR1C1	5465	ASY1655R	5'-Upstream	-48653 to -48507	-2153 to -2007	Chr22:46149731-46149877	14	202	0.595	500
	4-plex	RXRA	NR2B1	6256	ADS9116	Intron 1	36982 to 37088	37159 to 37265	Chr9:134363613-134363719	7	179	0.639	5000
	Ta = 59	RXRA	NR2B1	6256	ADS9105	Intron 1	31362 to 31469	31539 to 31646	Chr9:134357993-134358100	8	160	0.658	4000
		RXRA	NR2B1	6256	ADS9102	Intron 1	6834 to 6877	7011 to 7054	Chr9:134333465-134333508	6	103	0.719	1600
	N121P1	RARB	NR1B2	5915	ADS179	5'-Upstream	-529 to -503	-108 to -82	Chr3:25428203-25428229	3	133	0.9855	1500
	5-plex	SREBF1	SREBP1	6720	ASY1394	5'-Upstream	-420 to -368	-250 to -198	Chr17:17837237-17837185	8	121	0.6275	800
	Ta = 59	SREBF2	SREBP2	6721	ASY1397	5'-Upstream	-312 to -217	-146 to -111	Chr22:41832959-41832994	7	103	0.747	350
		TP53	p53	7157	ADS9168	3'-UTR	7521	18408	Chr17:7669074	1	91	0.98509413033875	300
		TP53	p53	7157	ADS9169	Exon 4	600	11487	Chr17:7675995	1	63	0.9891426309059	150
	N121P2	TP53	p53	7157	ADS5682	5'-Upstream	-11733 to -11597	-846 to -710	Chr17:7688327-7688191	12	193	0.838	2700
	4-plex	PTEN	MMAC1	5728	ADS3543	5'-Upstream	-2487 to -2405	-1130 to -1048	Chr10:87861983-87862065	8	172	0.986	1000
	Ta = 59	SREBF1	SREBP1	6720	ASY1393	5'-Upstream	-3224 to -3204	-3054 to -3034	Chr17:17840041-17840021	4	96	0.5855	2400
		SREBF2	SREBP2	6721	ASY1398	Exon 1	50 to 87	216 to 253	Chr22:41833320-41833357	5	85	0.8785	300
	N121P4	SREBF1	SREBP1	6720	ASY1395	Intron 1	439 to 642	609 to 812	Chr17:17836379-17836176	26	250	0.709	100
	4-plex	PPARGC1A	PGC1A	10891	ADS2154	5'-Upstream	-380 to -214	-260 to -94	Chr4:23890337-23890171	4	238	0.98	150
	Ta = 55	TP53	p53	7157	ADS5681	5'-Upstream	-11927 to -11755	-1040 to -868	Chr17:7688521-7688349	23	217	0.958	250
		TP53	p53	7157	ADS5683	5'-Upstream	-11095 to -10966	-208 to -79	Chr17:7687689-7687560	7	210	0.954	150
	N121P5	SREBF1	SREBP1	6720	ASY1396	Intron 1	12611 to 12796	12781 to 12966	Chr17:17824207-17824022	11	238	0.5845	150
	3-plex	SREBF1	SREBP1	6720	ASY1392	5'-Upstream	-3857 to -3766	-3687 to -3596	Chr17:17840674-17840583	8	198	0.7575	500
	Ta = 55	SREBF2	SREBP2	6721	ASY1399	Intron 1	575 to 709	741 to 875	Chr22:41833845-41833979	13	184	0.756	600
	N121P8	PPARG	NR1C3	5468	ADS2016B	Intron 1	-91777 to -91690	562 to 649	Chr3:12287929-12288016	15	132	0.9335	300
	2-plex Ta = 55	TP53	p53	7157	ADS5685	5'-UTR	-10848 to -10798	40 to 90	Chr17:7687442-7687392	7	107	0.961	250
	N121P9	SIRT1	SIR2	23411	ASY1541	Intron 1	509 to 667	575 to 733	Chr10:67885230-67885388	19	209	0.988	2100
	5-plex	SIRT1	SIR2	23411	ASY1543	Exon 4	6735 to 6775	6801 to 6841	Chr10:67891456-67891496	2	153	0.98758464062773	2000
	Ta = 53	SIRT1	SIR2	23411	ASY1540	5'-Upstream, 5'-UTR	-87 to -32	-21 to 35	Chr10:67884635-67884690	11	118	0.984	5400
		SIRT1	SIR2	23411	ASY1539	5'-Upstream	-394 to -352	-328 to -286	Chr10:67884328-67884370	4	108	0.97	3000
		SIRT1	SIR2	23411	ASY1542	Exon 3	4208 to 4233	4274 to 4299	Chr10:67888929-67888954	2	101	0.95049704200655	250
	13 PCRs	16 genes			58 assays					396 CpGs	8,898 bp

NGS134 - Fibromyalgia-related genes (Human)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N134P1	TNFRSF13B	TACI	23495	ASY1714	Exon 1, Intron 1	16 to 127	29 to 140	Chr17:16972060-16971949	7	226	0.991	150
	4-plex	TNFRSF13B	TACI	23495	ASY1713	5'-Upstream	-217 to -106	-204 to -93	Chr17:16972292-16972181	5	193	0.988	250
	Ta = 62	OXT	OT	5020	ASY1705	3'-Downstream	925 to 1003	961 to 1039	Chr20:3072580-3072658	9	140	0.825	250
		GRM2	GLUR2	2912	ASY1639	5'-Upstream	-2064 to -2044	-150 to -130	Chr3:51706920-51706940	3	70	0.648	100
	N134P2	OXT	OT	5020	ASY1700	5'-Upstream	-319 to -187	-283 to -151	Chr20:3071337-3071469	6	189	0.858	500
	6-plex	TRPA1	FEPS	8989	ASY1646	Exon 1, Intorn 1	77 to 206	252 to 381	Chr8:72075333-72075204	12	186	0.917	150
	Ta = 56	TRPA1	FEPS	8989	ASY1648	5'-Upstream	-688 to -620	-513 to -445	Chr8:72076097-72076029	4	172	0.987	250
		TRPA1	FEPS	8989	ASY1667	5'-Upstream	-281 to -193	-106 to -18	Chr8:72075690-72075602	13	148	0.984	200
		MCF2	DBL	4168	ASY1645	Intron 1	-40589 to -40500	15895 to 15984	ChrX:139692333-139692244	12	139	0.955	100
		AVP	ADH	551	ASY1706	5'-Upstream	-7292	-7242	Chr20:3091966	1	76	0.764	300
	N134P3	OXT	OT	5020	ASY1702	5'-Upstream, 5'-UTR, Exon 1	-49 to 54	-13 to 90	Chr20:3071607-3071709	10	165	0.675	150
	4-plex	TNFRSF13B	TACI	23495	ASY1715	Intron 1	223 to 260	236 to 273	Chr17:16971853-16971816	2	134	0.979	400
	Ta = 56	GRM2	GLUR2	2912	ASY1638	5'-Upstream	-2171 to -2104	-257 to -190	Chr3:51706813-51706880	9	121	0.552	1000
		IL25	IL17E	64806	ASY1644	5'-Upstream	-835 to -815	-577 to -557	Chr14:23372284-23372304	2	119	0.733	100
	N134P4	TRPA1	FEPS	8989	ADS2308	5'-Upstream	-1037 to -928	-862 to -753	Chr8:72076446-72076337	5	225	0.981	100
	7-plex	DRD3	D3DR	1814	ASY1625	5'-Upstream	-7033 to -6898	-327 to -192	Chr3:114179025-114178890	6	216	0.957	200
	Ta = 53	DRD3	D3DR	1814	ASY1621	5'-Upstream	-7771 to -7730	-1065 to -1024	Chr3:114179763-114179722	2	159	0.967	1800
		GRM2	GLUR2	2912	ASY1641	Intron 1	-1805 to -1714	110 to 201	Chr3:51707179-51707270	9	152	0.633	800
		OXT	OT	5020	ASY1701	5'-Upstream	-155 to -78	-119 to -42	Chr20:3071501-3071578	7	137	0.736	1800
		NR3C1	GCR	2908	ADS255	Intron 1	-2363 to -2288	488 to 563	Chr5:143403202-143403127	13	132	0.873	500
		AVP	ADH	551	ASY1707	5'-Upstream	-6064	-6014	Chr20:3090738	1	98	0.828	350
	N134P5	TRPA1	FEPS	8989	ASY1651	5'-Upstream	-379 to -320	-204 to -145	Chr8:72075788-72075729	7	147	0.953	100
	4-plex	TRPA1	FEPS	8989	ASY1647	5'-Upstream	-836	-661	Chr8:72076245	1	104	0.993	150
	Ta = 59	DRD3	D3DR	1814	ASY1622R	5'-Upstream	-7391	-685	Chr3:114179383	1	96	0.976	350
		C11orf40	NOV1	143501	ASY1709	5'-Upstream	-1091 to -1071	-1091 to -1071	Chr11:4578911-4578891	2	73	0.995	250
	N134P6	GCSAML	C1orf150	148823	ASY1636	Intron 1	-45189 to -45003	11351 to 11537	Chr1:247518408-247518594	24	240	0.944	150
	3-plex	C11orf40	NOV1	143501	ASY1711	Intron 1	1962 to 2091	1962 to 2091	Chr11:4575859-4575730	6	211	0.979	250
	Ta = 55	NR3C1	GCR	2908	ADS749	5'-Upstream	-3260 to -3204	-410 to -354	Chr5:143404099-143404043	7	100	0.721	150
	N134P7	MIR129-2	MIR-129b	406918	ADS8994	5'-Upstream	n/a	-250 to -87	Chr11:43581144-43581307	19	220	0.978	200
	2-plex Ta = 59	SYT2	SytII	127833	ASY1642R	Intron 1	-104127 to -104087	556 to 596	Chr1:202709899-202709859	5	102	0.861	500
	7 PCRs	13 genes			30 assays					210 CpGs	4,490 bp

NGS133 - Human OTX2 (orthodenticle homeobox 2)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N133P1	OTX2	MCOPS5	5015	ASY1601	Intron 2	-4693 to -4539	321 to 475	Chr14:56810149-56809995	11	221	0.741	1000
	4-plex	OTX2	MCOPS5	5015	ASY1605	Intron 2	-3917 to -3783	1097 to 1231	Chr14:56809373-56809239	16	199	0.746	3500
	Ta = 59	OTX2	MCOPS5	5015	ASY1603	Intron 2	-4375 to -4283	639 to 731	Chr14:56809831-56809739	7	159	0.813	900
		OTX2	MCOPS5	5015	ASY1611	Intron 2	-2914 to -2878	2100 to 2136	Chr14:56808370-56808334	6	102	0.93	2500
	N133P2	OTX2	MCOPS5	5015	ASY1608	Intron 2	-3382 to -3213	1632 to 1801	Chr14:56808838-56808669	13	254	0.827	100
	7-plex	OTX2	MCOPS5	5015	ASY1604	Intron 2	-4219 to -4050	795 to 964	Chr14:56809675-56809506	25	251	0.663	300
	Ta = 53	OTX2	MCOPS5	5015	ASY1606	Intron 2	-3751 to -3648	1263 to 1366	Chr14:56809207-56809104	15	170	0.887	700
		OTX2	MCOPS5	5015	ASY1610	Intron 2	-3059 to -2943	1955 to 1071	Chr14:56808515-56808399	13	164	0.789	10000
		OTX2	MCOPS5	5015	ASY1600	5'-Upstream	-5963 to -5896	-950 to -883	Chr14:56811419-56811352	8	150	0.868	2500
		OTX2	MCOPS5	5015	ASY1612	Intron 2	-2809 to -2708	2205 to 2306	Chr14:56808265-56808164	7	142	0.876	10000
		OTX2	MCOPS5	5015	ASY1602	Intron 2	-4503 to -4432	511 to 582	Chr14:56809959-56809888	9	129	0.848	700
	Simplex Ta = 55	OTX2	MCOPS5	5015	ASY1613	Intron 2	-2627 to -2435	2387 to 2579	Chr14:56808083-56807891	31	259	0.661	500
	3 PCRs	1 gene			12 assays					161 CpGs	2,200 bp

NGS123 - Human PMAIP1 (phorbol-12-myristate-13-acetate-induced protein 1)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N123P2	PMAIP1	NOXA	5366	ASY2056	5'-UTR	-206 to -68	25 to 163	Chr18:59899972-59900110	14	195	0.909	5000
	5-plex	PMAIP1	NOXA	5366	ASY2060	3'-UTR	2878 to 2905	3108 to 3135	Chr18:59903055-59903082	3	157	0.946	1000
	Ta = 55	PMAIP1	NOXA	5366	ASY2059	Intron 1	2228 to 2282	2458 to 2512	Chr18:59902405-59902459	3	131	0.918	7000
		PMAIP1	NOXA	5366	ASY2057	Intron 1	434 to 495	664 to 725	Chr18:59900611-59900672	6	118	0.923	10000
		PMAIP1	NOXA	5366	ASY2058	Intron 1	765 to 825	995 to 1055	Chr18:59900942-59901002	4	118	0.967	9000
	N123P5	PMAIP1	NOXA	5366	ASY2055	5'-Upstream	-1043 to -950	-813 to -720	Chr18:59899135-59899228	4	186	0.957	600
	4-plex	PMAIP1	NOXA	5366	ASY2054	5'-Upstream	-2513 to -2498	-2283 to -2268	Chr18:59897665-59897680	3	133	0.964	2500
	Ta = 50	PMAIP1	NOXA	5366	ASY2061	Exon 2	2480 to 2526	2710 to 2756	Chr18:59902657-59902703	2	132	0.954	2000
		PMAIP1	NOXA	5366	ASY2062	Intron 1	1457 to 1472	1687 to 1702	Chr18:59901634-59901649	2	110	0.9	3000
	2 PCRs	1 gene			9 assays					41 CpGs	1,280 bp

NGS104/087 - Human SRD5A1 & SRD5A2 (steroid 5 alpha-reductase 1 & 2)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N104P4	SRD5A1	S5AR1	6715	ADS5853	5'-Upstream, 5'-UTR	-345 to -159	-111 to 76	Chr5:6633232-6633418	28	247	0.801	150
	6-plex	SRD5A1	S5AR1	6715	ADS5860	Intron 4	33513 to 33638	33747 to 33872	Chr5:6667089-6667214	7	196	0.952	1300
	Ta = 53	SRD5A1	S5AR1	6715	ADS5858	Exon 2, Intron 2	18420 to 18562	18654 to 18796	Chr5:6651996-6652138	3	196	0.959	900
		SRD5A1	S5AR1	6715	ADS5857	Intron 1	1243 to 1309	1477 to 1543	Chr5:6634819-6634885	4	164	0.944	1800
		SRD5A1	S5AR1	6715	ADS5848	5'-Upstream	-1092 to -1038	-858 to -804	Chr5:6632485-6632539	6	137	0.785	2800
		SRD5A1	S5AR1	6715	ADS5859	Intron 3	26388 to 26430	26622 to 26664	Chr5:6659964-6660006	4	104	0.954	2500
	N104P8	SRD5A1	S5AR1	6715	ADS5850	5'-Upstream	-803 to -744	-569 to -510	Chr5:6632774-6632833	9	106	0.851	1200
	4-plex	SRD5A1	S5AR1	6715	ADS5852	5'-Upstream	-437 to -393	-203 to -159	Chr5:6633140-6633184	5	100	0.82	600
	Ta = 55	SRD5A1	S5AR1	6715	ADS5856	Intron 1	498 to 511	732 to 745	Chr5:6634074-6634087	2	65	0.931	100
		SRD5A1	S5AR1	6715	ADS5847	5'-Upstream	-1312 to -1310	-1078 to -1076	Chr5:6632265-6632267	2	63	0.951	100
	Simplex Ta = 55	SRD5A1	S5AR1	6715	ADS5855	Exon 1	188 to 245	422 to 479	Chr5:6633764-6633821	10	113	0.977	2000
	N087P1	SRD5A2	S5AR2	6716	ADS5438	Intron 1	481 to 580	648 to 747	Chr2:31580420-31580321	8	167	0.889	600
	3-plex	SRD5A2	S5AR2	6716	ADS5440	Intron 1	750 to 766	917 to 933	Chr2:31580151-31580135	3	134	0.989	1500
	Ta = 62	SRD5A2	S5AR2	6716	ADS5428	5'-Upstream	-1129 to -1124	-962 to -957	Chr2:31582029-31582024	2	120	0.991	350
	N087P2	SRD5A2	S5AR2	6716	ADS5429	5'-Upstream	-1040 to -899	-873 to -732	Chr2:31581940-31581799	10	210	0.948	900
	4-plex	SRD5A2	S5AR2	6716	ADS5439	Intron 1	635 to 676	802 to 843	Chr2:31580266-31580225	8	144	0.817	200
	Ta = 59	SRD5A2	S5AR2	6716	ADS5441	Intron 1	894 to 904	1061 to 1071	Chr2:31580007-31579997	2	142	0.974	2000
		SRD5A2	S5AR2	6716	ADS5433	5'-Upstream	-266 to -196	-99 to -29	Chr2:31581166-31581096	8	132	0.817	2400
	N087P3 2-plex	SRD5A2	S5AR2	6716	ADS5435	Exon 1	42 to 99	209 to 266	Chr2:31580859-31580802	7	113	0.963	500
	Ta = 59	SRD5A2	S5AR2	6716	ADS5431	5'-Upstream	-583 to -552	-416 to -385	Chr2:31581483-31581452	4	95	0.98	2600
	Simplex Ta = 56	SRD5A2	S5AR2	6716	ADS5436	Exon 1	125 to 228	292 to 395	Chr2:31580776-31580673	13	154	0.875	800
	7 PCRs	2 genes			21 assays					145 CpGs	2,902 bp

NGS100 - Human TP73 (tumor protein p73)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N100P1	TP73	p73	7161	ADS5788	5'-Upstream	-31732 to -31568	-1886 to -1722	Chr1:3650634-3650798	19	219	0.439	300
	5-plex	TP73	p73	7161	ASY9382	5'-Upstream	-32379 to -32270	-2533 to -2424	Chr1:3649987-3650096	17	174	0.516	1700
	Ta = 59	TP73	p73	7161	ADS2758	Intron 3	8168 to 8251	38014 to 38097	Chr1:3690533-3690616	9	155	0.818	1000
		TP73	p73	7161	ADS9374	5'-Upstream	-39048 to -38964	-9202 to -9118	Chr1:3643318-3643402	6	141	0.917	1500
		TP73	p73	7161	ADS2760	Intron 3	8291 to 8293	38137 to 38139	Chr1:3690656-3690658	2	92	0.758	500
	N100P3	TP73	p73	7161	ADS9373	5'-Upstream	-39330 to -39177	-9484 to -9331	Chr1:3643036-3643189	13	210	0.936	1600
	4-plex	TP73	p73	7161	ADS9381	5'-Upstream	-32515 to -32420	-2669 to -2574	Chr1:3649851-3649946	13	160	0.506	1200
	Ta = 56	TP73	p73	7161	ADS5790	5'-Upstream	-31416 to -31339	-1570 to -1493	Chr1:3650950-3651027	10	146	0.671	1000
		TP73	p73	7161	ADS9376	5'-Upstream	-37160 to -37106	-7314 to -7260	Chr1:3645206-3645260	3	121	0.758	1000
	N100P4	TP73	p73	7161	ASY9394	Intron 1	-29361 to -29271	486 to 576	Chr1:3653005-3653095	11	176	0.51	500
	6-plex	TP73	p73	7161	ASY9384	5'-Upstream	-31087 to -30972	-1241 to -1126	Chr1:3651279-3651394	16	173	0.432	1600
	Ta = 56	TP73	p73	7161	ASY9387	5'-Upstream	-30606 to -30486	-760 to -640	Chr1:3651760-3651880	12	168	0.481	750
		TP73	p73	7161	ASY9392	Intron 1	-29614 to -29525	233 to 322	Chr1:3652752-3652841	10	136	0.833	350
		TP73	p73	7161	ASY9383	5'-Upstream	-32186 to -32112	-2340 to -2266	Chr1:3650180-3650254	7	126	0.518	1000
		TP73	p73	7161	ADS9372	5'-Upstream	-39371 to -39362	-9525 to -9516	Chr1:3642995-3643004	2	89	0.855	3500
	N100P5	TP73	p73	7161	ASY9388	5'-Upstream	-30446 to -30282	-600 to -436	Chr1:3651920-3652084	16	218	0.561	150
	3-plex	TP73	p73	7161	ASY9386	5'-Upstream	-30785 to -30630	-939 to -784	Chr1:3651581-3651736	21	204	0.342	100
	Ta = 62	TP73	p73	7161	ASY9391	Intron 1	-29708 to -29638	139 to 209	Chr1:3652658-3652728	10	113	0.695	300
	N100P6	TP73	p73	7161	ADS5791	5'-Upstream	-31308 to -31122	-1462 to -1276	Chr1:3651058-3651244	20	240	0.547	600
	3-plex	TP73	p73	7161	ASY9379	5'-Upstream	-36582 to -36485	-6736 to -6639	Chr1:3645784-3645881	10	204	0.764	500
	Ta = 62	TP73	p73	7161	ASY9385	5'-Upstream	-30944 to -30806	-1098 to -960	Chr1:3651422-3651560	12	182	0.41	1000
	5 PCRs	1 gene			21 assays					239 CpGs	3,447 bp

NGS093 - Human T-cell receptor α locus	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N93P1	TRAV12-3	TCRAV2S2	28672	ADS9204	5'-Upstream, 5'-UTR	-64 to -43	-8 to 14	Chr14:21965443-21965464	2	156	0.841	1000
	5-plex	TRAJ59	n/a	28696	ADS9198	5'-Upstream	n/a	-442 to -363	Chr14:22476192-22476113	2	145	0.992	400
	Ta = 59	TRAV8-2	TCRAV1S5	28684	ADS9189	Intron 1	123 to 132	348 to 357	Chr14:21846884-21846893	2	143	0.88	500
		TRDJ4	n/a	28519	ADS9195	3'-Downstream	n/a	192 to 230	Chr14:22455478-22455441	3	142	0.993	100
		TRAV16	TCRAV9S1	28667	ADS9206	5'-Upstream	-101	-101	Chr14:21990395	1	140	0.91879027373335	1800
	N93P2	TRAV12-3	TCRAV2S2	28672	ADS9203	5'-Upstream	-247	-191	Chr14:21965260	1	148	0.87319439837255	350
	4-plex	TRDD3	TCRD	28523	ADS9194	5'-Upstream	n/a	-309 to -297	Chr14:22448818-22448806	2	147	0.985	200
	Ta = 59	TRDV2	n/a	28517	ADS9192	5'-Upstream	-702	-527	Chr14:22421846	1	131	0.92792520515717	2500
		TRAJ56	n/a	28699	ADS9200	5'-Upstream	n/a	-217 to -137	Chr14:22479386-22479306	3	131	0.996	500
	2 PCRs	6 genes			9 assays					17 CpGs	1,283 bp

NGS092 - Human ID3 (inhibitor of DNA binding 3)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N92P1	ID3	HEIR1	3399	ADS5614	5'-Upstream	-814	-446	Chr1:23560240	1	139	0.97151544957137	1400
	4-plex	ID3	HEIR1	3399	ADS9164	3'-Downstream	2579	2947	Chr1:23556848	1	129	0.97525327223557	400
	Ta = 59	ID3	HEIR1	3399	ADS5619	3'-Downstream	5033 to 5088	5401 to 5456	Chr1:23554394-23554339	5	126	0.938	100
		ID3	HEIR1	3399	ADS5618	Intron 2	528 to 575	896 to 943	Chr1:23558899-23558852	3	99	0.875	300
	N92P2	ID3	HEIR1	3399	ADS9163	3'-UTR, 3'-Downstream	1434 to 1595	1802 to 1963	Chr1:23557993-23557832	6	212	0.983	1500
	3-plex	ID3	HEIR1	3399	ADS5616	5'-UTR	-295 to -213	74 to 156	Chr1:23559721-23559639	11	149	0.899	300
	Ta = 62	ID3	HEIR1	3399	ADS5613	5'-Upstream	-2406	-2038	Chr1:23561832	1	106	0.95962669238101	500
	N92P3	ID3	HEIR1	3399	ADS5612	5'-Upstream	-3183 to -3035	-2815 to -2667	Chr1:23562609-23562461	16	241	0.755	150
	3-plex	ID3	HEIR1	3399	ADS5617	Exon 1	8 to 176	376 to 544	Chr1:23559419-23559251	18	224	0.808	100
	Ta = 56	ID3	HEIR1	3399	ADS9162	5'-Upstream	-2012 to -1901	-1644 to -1533	Chr1:23561438-23561327	3	180	0.927	800
	3 PCRs	1 gene			10 assays					65 CpGs	1,605 bp

NGS088 - Human OTC & CPS1 (Urea Cycle)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N88P1	OTC	OCTD	5009	ADS9140	5'-Upstream	-5215 to -5076	-5063 to -4924	ChrX:38347482-38347621	4	261	0.9847	500
	3-plex	OTC	OCTD	5009	ADS9142	5'-Upstream	-360 to -295	-208 to -143	ChrX:38352337-38352402	3	238	0.9804	800
	Ta = 53	CPS1	CPSASE1	1373	ADS5506	Exon 12, Intron 12	115145 to 115255	115200 to 115310	Chr2:210592908-210593018	4	197	0.9674	600
	N88P2	RPGR	RP3	6103	ADS5507	5'-Upstream	-870 to -773	-673 to -576	ChrX:38328237-38328140	3	169	0.8763	900
	5-plex	CPS1	CPSASE1	1373	ADS5493	5'-Upstream	-1573 to -1492	-1518 to -1437	Chr2:210476191-210476272	5	165	0.9305	200
	Ta = 50	RPGR	RP3	6103	ADS5509	5'-Upstream	-455 to -418	-258 to -221	ChrX:38327822-38327785	3	146	0.9523	400
		CPS1	CPSASE1	1373	ADS5497	5'-Upstream	-969 to -895	-914 to -840	Chr2:210476795-210476869	6	139	0.86	200
		RPGR	RP3	6103	ADS5512	Intron 16	51452 to 51471	51649 to 51668	ChrX:38275916-38275897	2	117	0.9847	500
	N88P3	CPS1	CPSASE1	1373	ADS5498	5'-Upstream	-847 to -673	-792 to -618	Chr2:210476917-210477091	4	239	0.9797	300
	3-plex	CPS1	CPSASE1	1373	ADS5502	Intron 1, Exon 2	78842 to 78975	78897 to 79030	Chr2:210556605-210556738	2	233	0.9766	5000
	Ta = 56	CPS1	CPSASE1	1373	ADS5496	5'-Upstream	-1143 to -1005	-1088 to -950	Chr2:210476621-210476759	13	204	0.9028	200
	N99P4	CPS1	CPSASE1	1373	ADS5501	Intron 1	78387 to 78488	78442 to 78543	Chr2:210556150-210556251	2	180	0.9809	8000
	2-plex Ta = 56	RPGR	RP3	6103	ADS5515	Intron 16	51963	52160	ChrX:38275405	1	75	0.995	3000
	Simplex Ta = 56	OTC	OCTD	5009	ADS9141	5'-Upstream	-717 to -641	-565 to -489	ChrX:38351980-38352056	6	172	0.9809	600
	Simplex Ta = 53	CPS1	CPSASE1	1373	ADS5505	Intron 11	114958	115013	Chr2:210592721	1	90	0.9851	10000
	6 PCRs	3 genes			15 assays					59 CpGs	2,625 bp

NGS081 - Human PDLIM4 & SLC22A4	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N81P1	PDLIM4	RIL	8572	ADS9129	5'-Upstream	-388 to -267	-324 to -203	Chr5:132257347-132257468	14	212	0.846	250
	2-plex Ta = 56	SLC22A4	OCTN1	6583	ADS9125	5'-Upstream	-800 to -730	-626 to -556	Chr5:132293817-132293887	6	87	0.957	1000
	N81P2	SLC22A4	OCTN1	6583	ADS9126	5'-Upstream	-969 to -881	-795 to -707	Chr5:132293648-132293736	11	186	0.954	400
	2-plex Ta = 56	PDLIM4	RIL	8572	ADS9122	Exon 5	13569 to 13626	13633 to 13690	Chr5:132271303-132271360	6	142	0.907	200
	N81P5	SLC22A4	OCTN1	6583	ADS5486	Intron 3	20723 to 20794	20897 to 20968	Chr5:132315339-132315410	3	152	0.974	350
	4-plex	PDLIM4	RIL	8572	ADS477B	5'-Upstream	-237 to -167	-173 to -103	Chr5:132257498-132257568	8	124	0.786	100
	Ta = 56	PDLIM4	RIL	8572	ADS5478	Exon 5, Intron 5	13722 to 13739	13786 to 13803	Chr5:132271456-132271473	4	113	0.961	500
		SLC22A4	OCTN1	6583	ADS5484	Intron 3	19611	19785	Chr5:132314227	1	102	0.97646123104554	350
	N81P6	SLC22A4	OCTN1	6583	ADS5483	Intron 3	19279 to 19323	19453 to 19497	Chr5:132313895-132313939	2	145	0.96	1300
	4-plex	SLC22A4	OCTN1	6583	ADS5485	Intron 3	19814 to 19868	19988 to 20042	Chr5:132314430-132314484	2	123	0.978	150
	Ta = 59	SLC22A4	OCTN1	6583	ADS5481	Intron 1	6983 to 7028	7157 to 7202	Chr5:132301599-132301644	3	122	0.991	500
		SLC22A4	OCTN1	6583	ADS5487	Intron 3	24265	24439	Chr5:132318881	1	101	0.96445279829537	300
	N81P8	SLC22A5	OCTN2	6584	ADS9127	5'-Upstream	-515 to -382	-294 to -161	Chr5:132369458-132369591	17	183	0.887	100
	3-plex	PDLIM4	RIL	8572	ADS9121B	Intron 1	372 to 385	436 to 449	Chr5:132258106-132258119	3	175	0.757	200
	Ta = 56	PDLIM4	RIL	8572	ADS9120	Intron 1	141 to 219	205 to 283	Chr5:132257875-132257953	9	127	0.922	200
	N81P9	SLC22A4	OCTN1	6583	ADS5470	5'-Upstream	-730 to -572	-556 to -398	Chr5:132293887-132294045	19	211	0.938	500
	2-plex Ta = 59	SLC22A4	OCTN1	6583	ADS5471	5'-Upstream	-376 to -244	-202 to -70	Chr5:132294241-132294373	15	155	0.959	350
	N81P11	SLC22A4	OCTN1	6583	ADS5472	5'-Upstream, 5'-UTR	-198 to -77	-24 to 98	Chr5:132294419-132294540	12	196	0.972	400
	2-plex Ta = 56	PDLIM4	RIL	8572	ADS9121A	Intron 1	265 to 339	329 to 403	Chr5:132257999-132258073	8	133	0.864	800
	Simplex Ta = 56	SLC22A4	OCTN1	6583	ADS5409	5'-Upstream	-574 to -376	-400 to -202	Chr5:132294043-132294241	22	249	0.839	150
	Simplex Ta = 56	SLC22A4	OCTN1	6583	ADS5482	Intron 1	7096 to 7249	7270 to 7423	Chr5:132301712-132301865	5	218	0.962	200
	9 PCRs	2 genes			21 assays					171 CpGs	3,256 bp

NGS070V3 - Human FOXP3 (forkhead box P3) Regulators	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N70V3P14	S100A6	CACY	6277	ADS1798	5'-Upstream	-1139 to -997	-256 to -114	Chr1:153,536,478-153,536,336	8	211	0.919	500
	8-plex	CTLA4	CD152	1493	ADS3074B	Exon 2	2651 to 2757	2808 to 2914	Chr2:203,870,593-203,870,699	5	193	0.989	500
	Ta = 60	IL4	BCGF-1	3565	ADS1623R	5'-Upstream, 5'-UTR	-144 to -12	-79 to 54	Chr5:132,673,907-132,674,039	4	191	0.931	350
		PDCD1	PD-1	5133	ADS2509	5'-Upstream	-438 to -359	-368 to -289	Chr2:241,859,276-241,859,197	4	182	0.842	150
		TNFRSF18	AITR	8784	ADS4562	Intron 4, Exon 5	2588 to 2705	2708 to 2825	Chr1:1,203,838-1,204,017	12	180	0.409	600
		ETS1	p54	2113	ADS3144	5'-Upstream, 5'-UTR	-14451 to -14376	-23 to 53	Chr11:128,587,472-128,587,630	3	157	0.993	500
		TNF	TNFA	7124	ADS711	5'-Upstream	-484 to -419	-307 to -242	Chr6:31,575,258-31,575,323	3	153	0.951	900
		FOXP3	JM2	50943	ADS6645A	5'-Upstream	-12155 to -12121	-5834 to -5800	ChrX:49,270,660-49,270,594	8	104	0.979	500
	N70V3P15	IKZF2	HELIOS	22807	ADS4563	Intron 4	27732 to 27748	30706 to 30722	Chr2:213,120,818-213,121,018	3	201	0.987	2500
	6-plex	FOXP3	JM2	50943	ADS783	Intron 1	-2376 to -2263	3946 to 4059	ChrX:49,260,881-49,260,768	11	181	0.877	400
	Ta = 58	PDCD1LG2	PDL2	80380	ADS5320	5'-Upstream	-12905 to -12852	-928 to -875	Chr9:5,510,497-5,510,595	3	170	0.948	1000
		IL2RB	CD122	3560	ASY1523	Intron 1	-2100 to -2033	3645 to 3712	Chr22:37,146,272-37,146,205	4	160	0.98	3000
		IL2RA	CD25	3559	ASY1521	Intron 4	24448 to 24517	24622 to 24691	Chr10:6,037,704-6,037,635	4	145	0.91	3500
		TOLLIP	IL-1RAcPIP	54472	ADS3481	Intron 5	31791 to 31833	31915 to 31957	Chr11:1,277,708-1,277,666	5	100	0.671	900
	N70V3P16	CD274	PD-L1	29126	ADS1929	Intron 1	-5312 to -5163	278 to 427	Chr9:5,450,802-5,450,951	5	228	0.957	200
	9-plex	RUNX1	AML1	861	ADS3030	5'-UTR, Intron 1	-308 to -238	92 to 162	Chr21:35,049,207-35,049,137	5	223	0.975	250
	Ta = 56	FOXP3	JM2	50943	ASY1524	Intron 1	-3452 to -3352	2870 to 2970	ChrX:49,261,957-49,261,857	5	207	0.889	600
		PDCD1LG2	PDL2	80380	ADS5321	5'-Upstream, 5'-UTR	-12050 to -11952	-73 to 26	Chr9:5,509,642-5,509,695	2	169	0.988	400
		TNFRSF25	DR3	8718	ADS319	5'-Upstream	-303 to -204	-235 to -136	Chr1:6,466,410-6,466,311	9	164	0.545	500
		IL2RB	CD122	3560	ASY1522	5'-Upstream	-23485 to -23409	-17741 to -17665	Chr22:37,167,657-37,167,581	4	153	0.797	1000
		FOXP3	JM2	50943	ASY1352re	Intron 1	-5378 to -5320	944 to 1002	ChrX:49,263,883-49,263,825	3	130	0.684	150
		FOXP3	JM2	50943	ADS8534A	5'-Upstream	-6451 to -6411	-130 to -90	ChrX:49,264,956-49,264,916	2	121	0.986	450
		PDCD1LG2	PDL2	80380	ADS5323	Intron 2	7569	19546	Chr9:5,530,115	1	77	0.92177953124908	1200
	N70V3P17	PDCD1	PD-1	5133	ADS2744	5'-Upstream	-858 to -739	-788 to -669	Chr2:241,859,696-241,859,577	5	187	0.719	600
	6-plex	IKZF4	EOS	64375	ADS4565	Intron 5	1969 to 2083	15804 to 15918	Chr12:56,023,462-56,023,576	2	179	0.805	350
	Ta = 53	CD3D/CD3G	CD3-δ/CD3-γ	915 & 917	ADS3513	5'-Upstream	-329 to -294	-261 to -226	Chr11:118,344,095-118,344,130	3	167	0.934	200
		CTLA4	CD152	1493	ASY1526	5'-Upstream	-31334 to -31249	-31177 to -31092	Chr2:203,836,609-203,836,694	6	155	0.977	10000
		CDKN1C	p57	1028	ADS1787	5'-Upstream	-946 to -876	-589 to -519	Chr11:2,886,402-2,886,332	11	129	0.651	250
		IL2	TCGF	3558	ADS740	5'-Upstream	-306	-21	Chr4:122,456,746	1	97	0.97906722390981	400
	4 PCRs	20 genes			29 assays					141 CpGs	4,714 bp

NGS061V1 - Human Neurology-related genes	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N61V1P7	NR3C1	GCR	2908	ASY2160	Intron 1	-1458 to -1320	1393 to 1531	Chr5:143402297-143402159	10	197	0.972	3200
	9-plex	SLC6A4	5-HTT	6532	ADS1818	Intron 1	-13359 to -13243	652 to 768	Chr17:30235317-30235201	5	195	0.9	500
	Ta = 59	FKBP5	P54	2289	ADS8775	Intron 8	52215	137974	Chr6:35590610	1	161	0.99517837545768	600
		SLC6A4	5-HTT	6532	ADS6867	3'-UTR	24816 to 24831	38826 to 38841	Chr17:30197143-30197128	2	147	0.986	1800
		FKBP5	P54	2289	ADS8771	Intron 2	-25455 to -25385	60305 to 60375	Chr6:35668279-35668209	2	137	0.99801368174345	4000
		OXTR	OT-R	5021	ADS1883B	Intron 1	-720 to -647	711 to 784	Chr3:8768907-8768834	6	126	0.862	6800
		NR3C1	GCR	2908	ADS5691	Intron 1	-301 to -263	2550 to 2588	Chr5:143401140-143401102	3	121	0.952	500
		FKBP5	P54	2289	ADS3267	Intron 2	-46602 to -46580	39158 to 39180	Chr6:35689426-35689404	2	90	0.947	1300
		BDNF	BULN2	627	ADS3873	Exon 2	189 to 195	62348 to 62354	Chr11:27658376-27658370	2	83	0.983	7500
	N61V1P8	FKBP5	P54	2289	ADS3137	Intron 2	-45100 to -44985	40660 to 40775	Chr6:35687924-35687809	10	188	0.923	200
	10-plex	BDNF	BULN2	627	ADS8772	Intron 1	-41332 to -41240	20828 to 20920	Chr11:27699896-27699804	7	177	0.848	1300
	Ta = 57	OXTR	OT-R	5021	ADS6556	Intron 3	5507 to 5550	6937 to 6980	Chr3:8762681-8762638	3	159	0.96289875204221	2500
		OXTR	OT-R	5021	ADS1883A	Intron 1	-861 to -775	570 to 656	Chr3:8769048-8768962	6	156	0.90164861514025	400
		NR3C1	GCR	2908	ASY2162	Intron 2	4063 to 4130	6913 to 6980	Chr5:143396777-143396710	2	147	0.982	3800
		NR3C1	GCR	2908	ADS750	5'-UTR	-2669 to -2584	182 to 267	Chr5:143403508-143403423	18	144	0.95	4700
		FKBP5	P54	2289	ADS3268	Intron 2	-46494 to -46395	39266 to 39365	Chr6:35689318-35689219	3	139	0.952	3900
		BDNF	BULN2	627	ADS5793	5'-Upstream	-62344 to -62325	-185 to -166	Chr11:27720908-27720889	2	117	0.86258482509019	9700
		NR3C1	GCR	2908	ADS2386	5'-Upstream	-3181 to -3125	-331 to -275	Chr5:143404020-143403964	8	116	0.806	3500
		AVP	ADH	551	ASY1706	5'-Upstream	-7292	-7242	Chr20:3091966	1	76	0.8170222873621	2100
	N61V1P9	BDNF	BULN2	627	ADS221	Intron 1	-43217 to -43049	18943 to 19111	Chr11:27701781-27701613	11	239	0.806	500
	9-plex	OXTR	OT-R	5021	ADS1882	5'-UTR, Intron 1	-1063 to -902	368 to 529	Chr3:8769250-8769089	11	227	0.854	100
	Ta = 55	FKBP5	P54	2289	ADS3828	Intron 8	52035 to 52163	137794 to 137922	Chr6:35590790-35590662	4	183	0.991	3400
		NR3C1	GCR	2908	ASY2161	Intron 1	-1198 to -195	1653 to 1756	Chr5:143402037-143401934	6	171	0.893	1200
		BDNF	BULN2	627	ADS3870	5'-Upstream	-64253 to -64169	-2094 to -2010	Chr11:27722817-27722733	9	137	0.873	1800
		NR3C1	GCR	2908	ADS1342	5'-Upstream	-3341 to -3275	-491 to -425	Chr5:143404180-143404114	10	112	0.98	800
		AVP	ADH	551	ASY1707	5'-Upstream	-6064	-6014	Chr20:3090738	1	98	0.8802262359255	500
		SLC6A4	5-HTT	6532	ADS3753	5'-Upstream	-15542 to -15529	-1532 to -1519	Chr17:30237500-30237487	3	78	0.915	700
		NR3C1	GCR	2908	ADS5690	Intron 1	-433	2418	Chr5:143401272	1	64	0.93458785223247	300
	3 PCRs	6 genes			28 assays					149 CpGs	3,985 bp

NGS006V1 - Human RUNX3 (Runt-related transcription factor 3)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (hg38)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N6V1P7	RUNX3	AML2	864	ADS4204	Intron 4	47355 to 47451	47794 to 47890	Chr1:24917217-24917121	5	175	0.899	300
	7-plex	RUNX3	AML2	864	ADS4225	3'-UTR	64719 to 64774	65158 to 65213	Chr1:24899853-24899798	3	150	0.733	200
	Ta = 62	RUNX3	AML2	864	ADS4173	Intron 1	33076 to 33164	33515 to 33603	Chr1:24931496-24931408	6	147	0.737	250
		RUNX3	AML2	864	ADS4124	Intron 1	3366 to 3391	3805 to 3830	Chr1:24961206-24961181	2	147	0.79	250
		RUNX3	AML2	864	ADS4216	Intron 5	58388 to 58437	58827 to 58876	Chr1:24906184-24906135	4	140	0.781	150
		RUNX3	AML2	864	ADS4146	Intron 1	16990 to 17051	17429 to 17490	Chr1:24947582-24947521	2	125	0.813	650
		RUNX3	AML2	864	ADS4228	3'-Downstream	67103 to 67154	67542 to 67593	Chr1:24897469-24897418	4	119	0.825	250
	N6V1P8	RUNX3	AML2	864	ADS4184	Exon 3, Intron 3	36967 to 37018	37406 to 37457	Chr1:24927605-24927554	6	195	0.638	200
	6-plex	RUNX3	AML2	864	ADS4207	Intron 4	48492 to 48547	48931 to 48986	Chr1:24916080-24916025	5	129	0.882	400
	Ta = 60	RUNX3	AML2	864	ADS4120	Intron 1	1730 to 1758	2169 to 2197	Chr1:24962842-24962814	3	114	0.822	1000
		RUNX3	AML2	864	ADS4117	Intron 1	621 to 665	1060 to 1104	Chr1:24963951-24963907	4	101	0.708	400
		RUNX3	AML2	864	ADS4142	Intron 1	12944 to 12990	13383 to 13429	Chr1:24951628-24951582	3	101	0.879	200
		RUNX3	AML2	864	ADS4155	Intron 1	27896 to 27920	28335 to 28359	Chr1:24936676-24936652	3	99	0.808	750
	N6V1P9	RUNX3	AML2	864	ADS4174	Intron 1	33213 to 33307	33652 to 33746	Chr1:24931359-24931265	14	159	0.684	400
	9-plex	RUNX3	AML2	864	ADS4125	Intron 1	3528 to 3555	3967 to 3994	Chr1:24961044-24961017	2	155	0.759	300
	Ta = 58	RUNX3	AML2	864	ADS4177	Intron 1	33802 to 33906	34241 to 34345	Chr1:24930770-24930666	10	153	0.618	200
		RUNX3	AML2	864	ADS4138	Intron 1	9655 to 9738	10094 to 10177	Chr1:24954917-24954834	3	146	0.878	350
		RUNX3	AML2	864	ADS4227	3'-Downstream	66481 to 66557	66920 to 66996	Chr1:24898091-24898015	5	144	0.793	200
		RUNX3	AML2	864	ADS4221	Intron 5	61289 to 61330	61728 to 61769	Chr1:24903283-24903242	4	132	0.834	150
		RUNX3	AML2	864	ADS4183	Exon 3	36859 to 36937	37298 to 37376	Chr1:24927713-24927635	8	131	0.671	800
		RUNX3	AML2	864	ADS4112	5'-Upstream	-684 to -621	-245 to -182	Chr1:24965255-24965192	7	130	0.768	150
		RUNX3	AML2	864	ADS4213	Intron 4	54848 to 54864	55287 to 55303	Chr1:24909724-24909708	3	110	0.815	450
	N6V1P10	RUNX3	AML2	864	ADS4153	Intron 1	27483 to 27636	27922 to 28075	Chr1:24937089-24936936	7	200	0.694	400
	6-plex	RUNX3	AML2	864	ADS4182	Intron 2	36464 to 36502	36903 to 36941	Chr1:24928108-24928070	3	199	0.676	100
	Ta = 58	RUNX3	AML2	864	ADS4139	Intron 1	9768 to 9896	10207 to 10335	Chr1:24954804-24954676	8	194	0.839	200
		RUNX3	AML2	864	ADS4222	Intron 5	61694 to 61756	62133 to 62195	Chr1:24902878-24902816	6	185	0.862	500
		RUNX3	AML2	864	ADS4143	Intron 1	14526 to 14583	14965 to 15022	Chr1:24950046-24949989	2	157	0.935	350
		RUNX3	AML2	864	ADS4162	Intron 1	30775 to 30855	31214 to 31294	Chr1:24933797-24933717	5	154	0.743	600
	4 PCRs	1 gene			28 assays					137 CpGs	4,091 bp

🐭 MOUSE

Assay Name	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm39)	# CpGs	Size (bp)	RSQ	Est. Cov.

NGS166 - Mouse Alb (Albumin)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm39)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N166P2	Alb	Alb1	11657	ASY3602	Exon 13, Intron 13	13674 to 13817	13712 to 13855	Chr5:90622467-90622610	5	206	0.989	600
	11-plex	Alb	Alb1	11657	ASY3594	Exon 8	7548 to 7612	7586 to 7650	Chr5:90616341-90616405	3	186	0.974	600
	Ta = 56	Alb	Alb1	11657	ASY3601	Intron 12	13392 to 13406	13430 to 13444	Chr5:90622185-90622199	2	174	0.973	400
		Alb	Alb1	11657	ASY3584	Intron 1	355 to 402	393 to 440	Chr5:90609148-90609195	3	170	0.985	200
		Alb	Alb1	11657	ASY3605	3'-Downstream	16549 to 16633	16587 to 16671	Chr5:90625342-90625426	3	155	0.988	600
		Alb	Alb1	11657	ASY3607	3'-Downstream	18951 to 19033	18989 to 19071	Chr5:90627744-90627826	2	118	0.995	200
		Alb	Alb1	11657	ASY3581	5'-Upstream	-2586 to -2524	-2548 to -2486	Chr5:90606208-90606270	2	138	0.999	200
		Alb	Alb1	11657	ASY3599	Intron 10	10256 to 10309	10294 to 10347	Chr5:90619049-90619102	2	120	0.986	400
		Alb	Alb1	11657	ASY3606	3'-Downstream	17817 to 17858	17855 to 17896	Chr5:90626610-90626651	2	151	0.995	2900
		Alb	Alb1	11657	ASY3604	3'-Downstream	16074 to 16110	16112 to 16148	Chr5:90624867-90624903	2	92	0.999	700
		Alb	Alb1	11657	ASY3579	5'-Upstream	-3621	-3583	Chr5:90605173	1	78	0.994	1400
	N166P3	Alb	Alb1	11657	ASY3608	3'-Downstream	20260	20298	Chr5:90629053	1	139	0.981	100
	8-plex	Alb	Alb1	11657	ASY3583	5'-Upstream	-184 to -110	-146 to -72	Chr5:90608610-90608684	2	136	0.975	4400
	Ta = 59	Alb	Alb1	11657	ASY3600	Intron 12	13084	13122	Chr5:90621877	1	131	0.995	1500
		Alb	Alb1	11657	ASY3598	Intron 9	9666	9704	Chr5:90618459	1	129	0.988	100
		Alb	Alb1	11657	ASY3582	5'-Upstream	-2017	-1979	Chr5:90606777	1	90	0.992	800
		Alb	Alb1	11657	ASY3590	Intron 4	3802	3840	Chr5:90612595	1	81	0.997	300
		Alb	Alb1	11657	ASY3585	Intron 1	655 to 659	693 to 697	Chr5:90609448-90609452	2	80	0.998	2100
		Alb	Alb1	11657	ASY3591	Exon 6	4979	5017	Chr5:90613772	1	80	0.997	6100
	N166P4	Alb	Alb1	11657	ASY3592	Intron 6	5850 to 6033	5888 to 6071	Chr5:90614643-90614826	4	239	0.995	800
	5-plex	Alb	Alb1	11657	ASY3596	Exon 9, Intron 9	8664 to 8783	8702 to 8821	Chr5:90617457-90617576	6	201	0.98	1300
	Ta = 59	Alb	Alb1	11657	ASY3588	Exon 3	1808 to 1877	1846 to 1915	Chr5:90610601-90610670	4	181	0.992	3800
		Alb	Alb1	11657	ASY3589	Exon 4	3092 to 3148	3130 to 3186	Chr5:90611885-90611941	3	162	0.995	1100
		Alb	Alb1	11657	ASY3597	Intron 9	9282	9320	Chr5:90618075	1	91	0.985	700
	Simplex Ta = 62	Alb	Alb1	11657	ASY3611	Intron 10, Exon 11	10993 to 11122	11031 to 11160	Chr5:90619786-90619915	3	184	0.984	2100
	4 PCRs	1 gene			25 assays					58 CpGs	3,512 bp

NGS161 - Mouse Evl (Ena-vasodilator stimulated phosphoprotein)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm39)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N161P1	Evl	n/a	14026	ASY3425	Intron 3	103273 to 103389	103758 to 103874	Chr12:108624740-108624856	5	203	0.973	100
	14-plex	Evl	n/a	14026	ASY3426	Intron 3	114979 to 115058	115464 to 115543	Chr12:108636446-108636525	4	194	0.943	300
	Ta = 62	Evl	n/a	14026	ASY3419	Intron 1	62574 to 62702	63059 to 63187	Chr12:108584041-108584169	5	185	0.988	50
		Evl	n/a	14026	ASY3401	Intron 1	50588 to 50629	51073 to 51114	Chr12:108572055-108572096	3	142	0.976	200
		Evl	n/a	14026	ASY3395	5'-UTR	-193 to -111	293 to 375	Chr12:108521275-108521357	18	138	0.798	400
		Evl	n/a	14026	ASY3422	Intron 1	86990	87475	Chr12:108608457	1	135	0.006	200
		Evl	n/a	14026	ASY3410	Intron 1	81011 to 81061	81496 to 81546	Chr12:108602478-108602528	2	113	0.98	300
		Evl	n/a	14026	ASY3420	Intron 1	80646 to 80673	81131 to 81158	Chr12:108602113-108602140	2	105	0.953	1600
		Evl	n/a	14026	ASY3412	Intron 3	102673	103158	Chr12:108624140	1	100	0.995	100
		Evl	n/a	14026	ASY3413	Exon 7	120941 to 120951	121426 to 121436	Chr12:108642408-108642418	2	98	0.939	200
		Evl	n/a	14026	ASY3403	Intron 1	50925 to 50938	51410 to 51423	Chr12:108572392-108572405	2	97	0.986	900
		Evl	n/a	14026	ASY3418	5'-Upstream	-2020 to -2014	-1535 tp -1529	Chr12:108519448-108519454	2	96	0.994	100
		Evl	n/a	14026	ASY3411	Intron 1	80415	80900	Chr12:108601882	1	89	0.845	100
		Evl	n/a	14026	ASY3417	5'-Upstream	-2233	-1748	Chr12:108519235	1	77	0.951	50
	N161P2	Evl	n/a	14026	ASY3409	Intron 1	67315 to 67407	67800 to 67892	Chr12:108588782-108588874	3	184	0.986	100
	10-plex	Evl	n/a	14026	ASY3396	Intron 1	12 to 100	497 to 585	Chr12:108521479-108521567	12	141	0.955	100
	Ta = 56	Evl	n/a	14026	ASY3427	Intron 11	130511 to 130575	130996 to 131060	Chr12:108651978-108652042	2	138	0.95	100
		Evl	n/a	14026	ASY3402	Intron 1	50786 to 50854	51271 to 51339	Chr12:108572253-108572321	3	137	0.973	100
		Evl	n/a	14026	ASY3399	Intron 1	37754 to 37796	38239 to 38281	Chr12:108559221-108559263	3	123	0.997	1500
		Evl	n/a	14026	ASY3400	Intron 1	50464 to 50477	50949 to 50962	Chr12:108571931-108571944	2	120	0.987	100
		Evl	n/a	14026	ASY3414	Exon 14, 3'-UTR	132854 to 132912	133339 to 133397	Chr12:108654321-108654379	5	114	0.9	500
		Evl	n/a	14026	ASY3408	Intron 1	66701 to 66727	67186 to 67212	Chr12:108588168-108588194	2	111	0.992	400
		Evl	n/a	14026	ASY3423	Intron 3	101430	101915	Chr12:108622897	1	70	0.972	200
		Evl	n/a	14026	ASY3416	5'-Upstream	-2429	-1944	Chr12:108519039	1	67	0.972	900
	N161P3	Evl	n/a	14026	ASY3391	5'-Upstream	-1091 to -995	-606 to -510	Chr12:108520377-108520473	4	225	0.988	100
	5-plex	Evl	n/a	14026	ASY3405	Intron 1	51278 to 51416	51763 to 51901	Chr12:108572745-108572883	6	217	0.988	100
	Ta = 57	Evl	n/a	14026	ASY3404	Intron 1	51012 to 51098	51497 to 51583	Chr12:108572479-108572565	3	204	0.981	300
		Evl	n/a	14026	ASY3407	Intron 1	65832 to 65956	66317 to 66441	Chr12:108587299-108587423	4	200	0.989	100
		Evl	n/a	14026	ASY3398	Intron 1	313 to 364	798 to 849	Chr12:108521780-108521831	3	143	0.959	50
	4 PCRs	1 gene			29 assays					103 CpGs	3,966 bp

NGS159 - Mouse Arc (activity regulated cytoskeletal-associated protein)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N159P1	Arc	Arg3.1	11838	ASY3351	Exon 1	289 to 433	487 to 631	Chr15:74672084-74671940	11	229	0.977	100
	6-plex	Arc	Arg3.1	11838	ASY3354	Exon 1	779 to 916	977 to 1114	Chr15:74671594-74671457	7	207	0.98	100
	Ta = 56	Arc	Arg3.1	11838	ASY3347	5'-Upstream	-380 to -278	-182 to -80	Chr15:74672752-74672650	14	150	0.975	100
		Arc	Arg3.1	11838	ASY3353	Exon 1	614 to 685	812 to 883	Chr15:74671759-74671688	4	130	0.966	3800
		Arc	Arg3.1	11838	ASY3350	Exon 1	24 to 66	222 to 264	Chr15:74672349-74672307	7	105	0.879	700
		Arc	Arg3.1	11838	ASY3345	5'-Upstream	-586 to -562	-388 to -364	Chr15:74672958-74672934	2	99	0.974	4200
	N159P2	Arc	Arg3.1	11838	ADS6522	Exon 1	103 to 256	301 to 454	Chr15:74672270-74672117	12	209	0.927	700
	4-plex	Arc	Arg3.1	11838	ASY3346	5'-Upstream	-483 to -419	-285 to -221	Chr15:74672855-74672791	5	146	0.995	300
	Ta = 51	Arc	Arg3.1	11838	ASY3355	5'-Upstream	-6990 to -6910	-6792 to -6712	Chr15:74679362-74679282	4	130	0.972	5100
		Arc	Arg3.1	11838	ASY3352	Exon 1	504 to 537	702 to 735	Chr15:74671869-74671836	3	100	0.937	2100
	2 PCRs	1 gene			10 assays					69 CpGs	1,505 bp

NGS156 - Mouse Jarid2 (jumonji, AT rich interactive domain 2)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N156P1	Jarid2	Jmj	16468	ASY3162	5'-Upstream	-1157 to -1122	-556 to -521	Chr13:44730715-44730750	4	115	0.865	700
	4-plex	Jarid2	Jmj	16468	ASY3170	Intron 2	108192 to 108219	108793 to 108820	Chr13:44840063-44840090	2	96	0.992	500
	Ta = 59	Jarid2	Jmj	16468	ASY3188	Exon 10	178525 to 178563	179126 to 179164	Chr13:44910396-44910434	3	111	0.972	300
		Jarid2	Jmj	16468	ASY3190	3'-UTR	188691 to 188728	189292 to 189329	Chr13:44920562-44920599	3	124	0.92	1300
	N156P2	Jarid2	Jmj	16468	ASY3160	5'-Upstream	-2211 to -2124	-1610 to -1523	Chr13:44729661-44729748	9	162	0.875	50
	4-plex	Jarid2	Jmj	16468	ASY3168	Intron 1	39152 to 39183	39753 to 39784	Chr13:44771023-44771054	3	157	0.962	50
	Ta = 59	Jarid2	Jmj	16468	ASY3185	Intron 8	174879 to 174908	175480 to 175509	Chr13:44906750-44906779	2	82	0.954	300
		Jarid2	Jmj	16468	ASY3186	Intron 9	176115 to 176136	176716 to 176737	Chr13:44907986-44908007	2	93	0.98	400
	N156P3	Jarid2	Jmj	16468	ASY3163	5'-Upstream	-704 to -649	-103 to -48	Chr13:44731168-44731223	6	131	0.964	600
	4-plex	Jarid2	Jmj	16468	ASY3167	Intron 1	35922 to 35937	36523 to 36538	Chr13:44767793-44767808	2	94	0.99	2000
	Ta = 59	Jarid2	Jmj	16468	ASY3179	Intron 4	148554	149155	Chr13:44880425	1	74	0.987	300
		Jarid2	Jmj	16468	ASY3180	Intron 5	158766	159367	Chr13:44890637	1	119	0.993	600
	N156P4	Jarid2	Jmj	16468	ASY3166	Intron 1	2149 to 2235	2750 to 2836	Chr13:44734020-44734106	9	156	0.961	100
	4-plex	Jarid2	Jmj	16468	ASY3169	Intron 1	48158	48759	Chr13:44780029	1	61	0.989	100
	Ta = 59	Jarid2	Jmj	16468	ASY3178	Intron 3	125037 to 125120	125638 to 125721	Chr13:44856908-44856991	3	139	0.998	2200
		Jarid2	Jmj	16468	ASY3184	Intron 7	172605	173206	Chr13:44904476	1	132	0.936	900
	N156P5	Jarid2	Jmj	16468	ASY3164	Exon 1, Intron 1	45 to 89	646 to 690	Chr13:44731916-44731960	2	136	0.907	400
	5-plex	Jarid2	Jmj	16468	ASY3173	Intron 2	110157 to 110181	110758 to 110782	Chr13:44842028-44842052	2	134	0.996	50
	Ta = 53	Jarid2	Jmj	16468	ASY3175	Intron 2	112064 to 112117	112665 to 112718	Chr13:44843935-44843988	3	184	0.996	50
		Jarid2	Jmj	16468	ASY3176	Intron 3	119607 to 119651	120208 to 120252	Chr13:44851478-44851522	2	137	0.99	300
		Jarid2	Jmj	16468	ASY3181	Intron 5	159670 to 159719	160271 to 160320	Chr13:44891541-44891590	2	103	0.987	300
	Simplex Ta = 59	Jarid2	Jmj	16468	ASY3159	5'-Upstream	-2453 to -2351	-1852 to -1750	Chr13:44729419-44729521	6	169	0.865	1900
	6 PCRs	1 gene			22 assays					69 CpGs	2,709 bp

NGS154 - Mouse Pdgfb (platelet derived growth factor) & Mapk1 (mitogen-activated protein kinase 1)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N154P1	Pdgfb	Sis	18591	ASY3114	5'-Upstream	-5503 to -5387	-4693 to -4577	Chr15:80019670-80019554	6	215	0.913	600
	6-plex	Mapk1	Erk	26413	ASY3108	Intron 1	22623 to 22698	22868 to 22943	Chr16:17006249-17006324	2	173	0.957	100
	Ta = 56	Pdgfb	Sis	18591	ASY3118	Intron 4	13408 to 13472	14218 to 14282	Chr15:80000760-80000696	5	152	0.907	1700
		Mapk1	Erk	26413	ASY3106	Intron 1	13028 to 13066	13273 to 13311	Chr16:16996654-16996692	2	114	0.996	800
		Pdgfb	Sis	18591	ASY3117	Intron 1	7578 to 7621	8388 to 8431	Chr15:80006590-80006547	3	92	0.962	200
		Mapk1	Erk	26413	ASY3107	Intron 1	20085 to 20108	20330 to 20353	Chr16:17003711-17003734	2	84	0.995	6800
	N154P2	Mapk1	Erk	26413	ASY3104	5'-Upstream	-580 to -490	-335 to -245	Chr16:16983047-16983137	4	163	0.965	1700
	6-plex	Pdgfb	Sis	18591	ASY3116	Intron 1	756 to 849	1566 to 1659	Chr15:80013412-80013319	9	146	0.86	900
	Ta = 59	Pdgfb	Sis	18591	ASY3115	5'-Upstream	-1198 to -1144	-388 to -334	Chr15:80015365-80015311	2	144	0.944	4900
		Mapk1	Erk	26413	ASY3110	Intron 1	28247 to 28291	28492 to 28536	Chr16:17011873-17011917	2	105	0.986	1200
		Mapk1	Erk	26413	ASY3112	Intron 1	10785	11030	Chr16:16994411	1	96	0.997	9100
		Mapk1	Erk	26413	ASY3105	Intron 1	530 to 560	775 to 805	Chr16:16984156-16984186	4	90	0.956	1500
	Simplex Ta = 62	Mapk1	Erk	26413	ASY3113	5'-Upstream, 5'-UTR	-398 to -208	-153 to 38	Chr16:16983229-16983419	22	272	0.783	100
	3 PCRs	2 genes			13 assays					64 CpGs	1,846 bp

NGS150V1 - Mouse Elovl2 (elongation of very long chain fatty acids 2)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N150V1P1	Elovl2	Ssc2	54326	ASY2900	Intron 2	23911 to 24061	23959 to 24109	Chr13:41196447-41196297	4	201	0.917	100
	9-plex	Elovl2	Ssc2	54326	ASY2906	Intron 1	7395 to 7447	7443 to 7495	Chr13:41212963-41212911	3	151	0.935	1800
	Ta = 62	Elovl2	Ssc2	54326	ASY2861	5'-Upstream	-467 to -412	-419 to -364	Chr13:41220824-41220769	6	146	0.973	400
		Elovl2	Ssc2	54326	ASY2894	Exon 2, Intron 2	23220 to 23265	23268 to 23313	Chr13:41197138-41197093	2	142	0.972	1700
		Elovl2	Ssc2	54326	ASY2896	Exon 4, Intron 4	28443 to 28514	28491 to 28562	Chr13:41191915-41191844	3	138	0.955	200
		Elovl2	Ssc2	54326	ASY2905	Intron 1	16952 to 17020	17000 to 17068	Chr13:41203406-41203338	3	130	0.98	1400
		Elovl2	Ssc2	54326	ASY2907	Intron 1	7664 to 7695	7712 to 7743	Chr13:41212694-41212663	2	106	0.928	1500
		Elovl2	Ssc2	54326	ASY2898	Exon 8, 3'-UTR	35045 to 35075	35093 to 35123	Chr13:41185313-41185283	2	81	0.87	2700
		Elovl2	Ssc2	54326	ASY2902	3'-Downstream	39620	39668	Chr13:41180738	1	80	0.82340796632375	3300
	N150V1P2	Elovl2	Ssc2	54326	ASY2890	Intron 1	294 to 424	342 to 472	Chr13:41220064-41219934	9	197	0.984	1200
	7-plex	Elovl2	Ssc2	54326	ASY2888	5'-Upstream	-365 to -255	-317 to -207	Chr13:41220722-41220612	18	164	0.981	600
	Ta = 59	Elovl2	Ssc2	54326	ASY2885	5'-Upstream	-678 to -614	-630 to -566	Chr13:41221035-41220971	3	134	0.956	1600
		Elovl2	Ssc2	54326	ASY2892	Intron 1	1941 to 1992	1989 to 2040	Chr13:41218417-41218366	3	130	0.958	3200
		Elovl2	Ssc2	54326	ASY2887	5'-Upstream	-879 to -853	-831 to -805	Chr13:41221236-41221210	3	101	0.903	400
		Elovl2	Ssc2	54326	ASY2891	Intron 1	838 to 852	886 to 900	Chr13:41219520-41219506	2	97	0.934	9000
		Elovl2	Ssc2	54326	ASY2886	5'-Upstream	-777	-729	Chr13:41221134	1	78	0.83454563916134	500
	2 PCRs	1 gene			16 assays					65 CpGs	2,076 bp

NGS146 - Mouse CNS (central nervous system)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N146P1	Oxtr	Otr	18430	ASY2807	Exon 1	694	840	Chr6:112489104-112489026	12	207	0.55	100
	7-plex	Grin2b	Nr2b	14812	ADS662	Intron 1	-128023 to -127927	802 to 898	Chr6:136172324-136172228	12	154	0.857	500
	Ta = 62	Fkbp5	Dit	14229	ADS3545	Intron 4	-257 to -212	76236 to 76281	Chr17:28441289-28441244	3	136	0.717	250
		Crh	Crf	12918	ADS444	Intron 1	-211 to -150	710 to 771	Chr3:19694687-19694626	7	126	0.681	200
		Foxp2	Spch1	114142	ADS1916	Intron 3	-15399 to -15363	280214 to 280250	Chr6:15181562-15181598	4	124	0.605	400
		Sox1	Sox	20664	ASY2814	5'-Upstream	-1481 to -1423	-415 to -357	Chr8:12394880-12394938	5	120	0.621	300
		Atrx	Xh2	22589	ADS1540B	5'-Upstream	-547 to -522	-263 to -238	ChrX:105929666-105929641	2	118	0.762	300
	N146P2	Oxtr	Otr	18430	ASY2805	5'-Upstream	-989 to -858	-843 to -712	Chr6:112490786-112490655	9	193	0.478	100
	7-plex	Oxtr	Otr	18430	ASY2806	5'-UTR, Exon 1	-86 to 45	61 to 191	Chr6:112489883-112489753	15	148	0.615	200
	Ta = 59	Oxtr	Otr	18430	ASY2804	5'-Upstream	-1360 to -1296	-1214 to -1150	Chr6:112491157-112491093	8	136	0.532	450
		Nr3c1	Gcr	14815	ADS5418	Intron 1	-3006 to -2946	437 to 497	Chr18:39490238-39490178	6	118	0.68	300
		Ezh2	Enx1	14056	ASY2822	5'-Upstream, 5'-UTR	-17389 to -17343	-19 to 28	Chr6:47595049-47595003	9	104	0.863	250
		Mecp2	Mbd5	17257	ADS5416A	Intron 1	-29036	26394	ChrX:74108970	1	101	0.86832063536319	300
		Bdnf	Buln2	12064	ADS135A	Intron 1	16895	17532	Chr2:109692231	1	73	0.60073476688197	450
	N146P3	Bdnf	Buln2	12064	ADS135B	Intron 1	16952 to 17013	17589 to 17650	Chr2:109692288-109692349	4	159	0.577	200
	7-plex	Sox1	Sox	20664	ASY2813	5'-Upstream	-2265 to -2183	-1199 to -1177	Chr8:12394096-12394178	5	132	0.658	150
	Ta = 56	Gfra1	Gdnfr	14585	ADS932	5'-UTR	-949 to -895	102 to 156	Chr19:58455297-58455243	5	118	0.738	750
		Pde4d	Dpde3	238871	ADS687	Intron 3	825793 to 825843	1031782 to 1031832	Chr13:109685958-109686008	5	109	0.85	350
		Sox1	Sox	20664	ASY2826	Exon 1	148 to 199	1214 to 1265	Chr8:12396508-12396559	7	103	0.622	100
		Ezh2	Enx1	14056	ASY2824	Intron 1	-16641 to -16604	730 to 767	Chr6:47594301-47594264	6	86	0.663	200
		Nr3c2	Mcr	110784	ADS5423	Intron 3	189513	198013	Chr8:77097784	1	86	0.83535209137215	300
	N146P4	Fkbp5	Dit	14229	ADS5316	Intron 4	-867 to -739	75626 to 75754	Chr17:28441899-28441771	3	193	0.712	200
	7-plex	Bdnf	Buln2	12064	ADS431B	Intron 1	17065 to 17164	17702 to 17801	Chr2:109692401-109692500	8	178	0.642	500
	Ta = 53	Ezh2	Enx1	14056	ASY2825	Intron 1	-16182 to -16107	1189 to 1264	Chr6:47593842-47593767	5	145	0.747	1700
		Nr3c1	Gcr	14815	ADS5419A	Intron 1	-2920 to -2832	523 to 611	Chr18:39490152-39490064	20	130	0.609	250
		Bdnf	Buln2	12064	ADS5403B	Intron 1	16751	17388	Chr2:109692087	1	111	0.63946055974714	750
		Crh	Crf	12918	ADS310A	5'-Upstream	-1091 to -1065	-171 to -145	Chr3:19695567-19695541	4	105	0.625	100
		Nr3c1	Gcr	14815	ADS5421	Intron 2	45874	49316	Chr18:39441359	1	105	0.75991189168598	200
	4 PCRs	14 genes			28 assays					169 CpGs	3,618 bp

NGS137V2 - Mouse Imprinted Genes	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N137V2P6	Kcnq1ot1	Lit1	63830	ADS913	Exon 1	n/a	1208 to 1360	Chr7:143295342-143295190	9	209	0.963	200
	10-plex	Mest	Peg1	17294	ASY1686	5'-Upstream	-701 to -559	-441 to -299	Chr6:30737609-30737751	7	207	0.939	400
	Ta = 58	Peg3	Pw1	18616	ADS190	5'-UTR, Intron 1	-11901 to -11765	80 to 216	Chr7:6730342-6730206	11	192	0.991	800
		H19	Asm	14955	ADS445	5'-Upstream	n/a	-2643 to -2574	Chr7:142580786-142580717	6	181	0.68	300
		IG-DMR	n/a	n/a	ADS1452S	Intergenic	n/a	n/a	Chr12:109528375-109528471	18	175	0.89	100
		Sgce	e-SG	20392	ASY1684	Exon 1, Intron 1	47 to 126	135 to 214	Chr6:4747042-4746963	7	152	0.966	300
		Zdbf2	Slx9	73884	ASY1814	5'-Upstream	-27100 to -27040	-9627 to -9567	Chr1:63263638-63263698	5	149	0.987	200
		Peg3	Pw1	18616	ASY1691	5'-Upstream	-12159 to -12065	-179 to -85	Chr7:6730600-6730506	6	145	0.985	300
		Rasgrf1	Gnrp	19417	ASY1722	5'-Upstream	-34119 to -34085	-34030 to -33996	Chr9:89875879-89875913	3	113	0.939	1000
		Snrpn	Peg4	20646	ASY1694	Intron 2	-16297 to -16249	211 to 259	Chr7:60004901-60004853	6	105	0.991	400
	N137V2P7	Peg13	n/a	353342	ASY1806	Exon 1	n/a	697 to 842	Chr15:72809627-72809482	12	223	0.886	100
	9-plex	Mir127	n/a	387146	ADS1374	Exon 1, 3’-Downstream	n/a	43 to 169	Chr12:109592889-109593015	8	196	0.926	100
	Ta = 54	Rasgrf1	Gnrp	19417	ASY1721	5'-Upstream	-35491 to -35386	-35402 to -35297	Chr9:89874507-89874612	4	171	0.92	200
		Airn	Air	104103	ASY1809R	Intron 1	n/a	1362 to 1189	Chr17:12742271-12742174	10	164	0.972	900
		Mest	Peg1	17294	ADS915B	5'-Upstream	-1294 to -1249	-1034 to -989	Chr6:30737016-30737061	3	123	0.885	400
		Peg13	n/a	353342	ASY1807	Exon 1	n/a	997 to 1052	Chr15:72809327-72809272	4	115	0.843	100
		Nespas	Gnas	14683	ASY1679	Intron 1	n/a	377 to 410	Chr2:174295026-174295059	5	110	0.966	100
		Sgce	e-SG	20392	ASY1682	5'-Upstream	-2070 to -2050	-1982 to -1962	Chr6:4749158-4749138	7	86	0.832	500
		Grb10	Meg1	14783	ASY1803	Intron 1	-55144 to -55122	11642 to 11664	Chr11:12025760-12025738	2	83	0.972	900
	N137V2P8	Airn	Air	104103	ASY1810R	Intron 1	n/a	931 to 834	Chr17:12742702-12742529	8	237	0.987	200
	9-plex	IG-DMR	n/a	n/a	ADS8998B	Intergenic	n/a	n/a	Chr12:109526596-109526736	9	236	0.875	200
	Ta = 51	Sgce	e-SG	20392	ASY1683	5'-Upstream	-780 to -648	-692 to -560	Chr6:4747868-4747736	15	183	0.834	100
		Gnasxl	Gnas	14683	ASY1680	5'-Upstream	-1426 to -1318	-1423 to -1315	Chr2:174296436-174296544	8	158	0.883	100
		Zdbf2	Slx9	73884	ASY1716	5'-Upstream	-26927 to -26879	-9454 to -9406	Chr1:63263811-63263859	4	146	0.96	600
		Plagl1	Zac1	22634	ADS191	Intron 1	-33977 to -33890	253 to 340	Chr10:13091040-13091127	13	137	0.993	600
		Mest	Peg1	17294	ASY1687	Exon 1, Intron 1	7 to 86	267 to 346	Chr6:30738316-30738395	10	133	0.881	800
		Impact	Rwdd5	16210	ASY1812	Intron 1	2120 to 2186	2534 to 2600	Chr18:12974456-12974522	5	118	0.953	200
		Igf2r	Cd222	16004	ADS2710re	5'-Upstream	-432 to -400	-301 to -269	Chr17:12769965-12769933	4	83	0.912	400
	N137V2P9	Kcnq1ot1	Lit1	63830	ADS912	Exon 1	n/a	1041 to 1208	Chr7:143295509-143295342	12	264	0.953	200
	9-plex	Dlk1	Peg9	13386	ADS6547	Exon 5	6050 to 6206	6255 to 6411	Chr12:109459709-109459865	16	239	0.917	100
	Ta = 56	Nesp	Gnas	14683	ADS3759	5'-Upstream	-639 to -497	-285 to -143	Chr2:174284035-174284177	7	221	0.936	100
		Gnas	n/a	14683	ADS3756	5'-Upstream	-1906 to -1752	-1616 to -1462	Chr2:174328390-174328544	17	216	0.849	100
		H19	Asm	14955	ADS447	5'-Upstream	n/a	-2446 to -2358	Chr7:142580589-142580501	6	212	0.65	1000
		Igf2	Peg2	16002	ADS2052	5'-Upstream	-13921 to -13824	-12235 to -12138	Chr7:142669731-142669634	9	203	0.969	400
		IG-DMR	n/a	n/a	ADS1373	Intergenic	n/a	n/a	Chr12:109530388-109530457	7	196	0.93	200
		H19	Asm	14955	ADS1702	5'-Upstream	n/a	-3737 to -3618	Chr7:142581880-142581761	9	183	0.53	100
		Meg3	Gtl2	17263	ADS1341	Intron 1	n/a	713 to 807	Chr12:109541718-109541812	7	163	0.371	700
	4 PCRs	20 genes			37 assays					298 CpGs*	6,227 bp

NGS129 - Skeletal Muscle-related genes (Mouse)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N129P1	Ubr5	Edd1	70790	ASY2368	Intron 1	26383 to 26419	26383 to 26419	Chr15:38052043-38052007	3	146	0.99	500
	6-plex	Agtr1a	At1	11607	ASY2301	Intron 1	-38656 to -38618	5858 to 5896	Chr13:30342298-30342336	2	132	0.981	200
	Ta = 62	Stag1	Sa1	20842	ASY2339	5'-Upstream	-62514 to -62473	-736 to -695	Chr9:100642712-100642753	4	113	0.989	100
		Bicc1	Bicc	83675	ASY2312	Intron 1	3887 to 3940	3971 to 4024	Chr10:71155730-71155677	5	109	0.952	600
		Agtr1a	At1	11607	ASY2295	5'-Upstream	-44567 to -44514	-54 to -1	Chr13:30336387-30336440	4	106	0.964	800
		Ubr5	Edd1	70790	ASY2365	Intron 1	12327 to 12360	12327 to 12360	Chr15:38066099-38066066	3	90	0.988	300
	N129P2	Traf1	Ebi6	22029	ASY2355	Intron 3	1649 to 1740	5154 to 5245	Chr2:34956432-34956341	5	186	0.98	100
	8-plex	Ubr5	Edd1	70790	ASY2364	Intron 1	11719 to 11832	11719 to 11832	Chr15:38066707-38066594	4	174	0.992	300
	Ta = 59	Odf2	Odf84	18286	ASY2331	Intron 2	-1461 to -1391	1692 to 1762	Chr2:29891586-29891656	3	164	0.927	400
		Stag1	Sa1	20842	ASY2337	5'-Upstream	-62858 to -62796	-1080 to -1018	Chr9:100642368-100642430	3	152	0.985	100
		Odf2	Odf84	18286	ASY2329	5'-UTR, Intron 2	-2115 to -2025	1038 to 1128	Chr2:29890932-29891022	4	146	0.889	100
		Ubr5	Edd1	70790	ASY2358	5'-Upstream	-1129 to -1055	-1129 to -1055	Chr15:38079554-38079480	7	139	0.991	100
		Odf2	Odf84	18286	ASY2324	5'-Upstream	-3897 to -3835	-745 to -683	Chr2:29889150-29889212	5	135	0.921	400
		Traf1	Ebi6	22029	ASY2348	5'-Upstream	-5227 to -5172	-1722 to -1667	Chr2:34963307-34963252	2	117	0.925	100
	N129P3	Stag1	Sa1	20842	ASY2343	Intron 1	-60643 to -60493	1136 to 1286	Chr9:100644583-100644733	8	201	0.986	100
	11-plex	Ubr5	Edd1	70790	ASY2363	Intron 1	9991 to 10125	9991 to 10125	Chr15:38068435-38068301	6	189	0.964	100
	Ta = 59	Traf1	Ebi6	22029	ASY2344	5'-Upstream	-7824 to -7713	-4319 to -4208	Chr2:34965904-34965793	5	168	0.897	400
		Agtr1a	At1	11607	ASY2300	Intron 1	-38268 to -38179	6246 to 6335	Chr13:30342686-30342775	7	164	0.918	100
		Grik2	Glur6	14806	ASY2315	5'-Upstream	-7006 to -6913	-1499 to -1406	Chr10:49790265-49790172	3	153	0.971	100
		Ubr5	Edd1	70790	ASY2366	Intron 1	15221 to 15305	15221 to 15305	Chr15:38063205-38063121	4	148	0.986	500
		Agtr1a	At1	11607	ASY2297	Intron 1	-44270 to -44194	244 to 320	Chr13:30336684-30336760	6	147	0.976	150
		Bicc1	Bicc	83675	ASY2307	5'-Upstream	-598 to -539	-514 to -455	Chr10:71160214-71160155	3	144	0.955	1200
		Bicc1	Bicc	83675	ASY2311	Intron 1	3446 to 3514	3530 to 3598	Chr10:71156171-71156103	3	142	0.943	150
		Grik2	Glur6	14806	ASY2323	Intron 2	4555 to 4586	10062 to 10093	Chr10:49778705-49778674	3	136	0.954	100
		Traf1	Ebi6	22029	ASY2349	5'-Upstream	-5048 to -4978	-1543 to -1473	Chr2:34963128-34963058	3	129	0.916	400
	N129P4	Odf2	Odf84	18286	ASY2335	Exon 5	410 to 500	3562 to 3652	Chr2:29893456-29893546	7	176	0.928	250
	8-plex	Agtr1a	At1	11607	ASY2304	Intron 2	-35235 to -35141	9279 to 9373	Chr13:30345719-30345813	6	154	0.947	200
	Ta = 56	Bicc1	Bicc	83675	ASY2310	Intron 1	2787 to 2856	2871 to 2940	Chr10:71156830-71156761	3	154	0.953	1100
		Grik2	Glur6	14806	ASY2316	5'-Upstream	-5738 to -5653	-231 to -146	Chr10:49788997-49788912	5	151	0.991	600
		Agtr1a	At1	11607	ASY2298	Intron 1	-42388 to -42304	2126 to 2210	Chr13:30338566-30338650	4	131	0.918	350
		Ubr5	Edd1	70790	ASY2362	Intron 1	1222 to 1266	1222 to 1266	Chr15:38077204-38077160	4	116	0.951	150
		Odf2	Odf84	18286	ASY2330	Intron 2	-1633 to -1594	1520 to 1559	Chr2:29891414-29891453	5	106	0.856	200
		Odf2	Odf84	18286	ASY2328	Intron 1	-2695 to -2660	458 to 493	Chr2:29890352-29890387	5	105	0.879	100
	N129P5	Agtr1a	At1	11607	ASY2302	Intron 1	-36681 to -36609	7833 to 7905	Chr13:30344273-30344345	5	200	0.986	250
	7-plex	Grik2	Glur6	14806	ASY2313	5'-Upstream	-7517 to -7429	-2010 to -1922	Chr10:49790776-49790688	3	190	0.961	100
	Ta = 59	Stag1	Sa1	20842	ASY2341	5'-UTR	-61565 to -61445	214 to 334	Chr9:100643661-100643781	15	168	0.961	100
		Grik2	Glur6	14806	ASY2322	Intron 2	4191 to 4298	9698 to 9805	Chr10:49779069-49778962	4	165	0.965	500
		Odf2	Odf84	18286	ASY2334	Intron 3	-326 to -223	2827 to 2930	Chr2:29892721-29892824	4	157	0.92	600
		Traf1	Ebi6	22029	ASY2354	Intron 3	860 to 927	4365 to 4432	Chr2:34957221-34957154	3	123	0.965	400
		Ubr5	Edd1	70790	ASY2361	Intron 1	1104 to 1170	1104 to 1170	Chr15:38077322-38077256	6	119	0.939	150
	N129P6	Ubr5	Edd1	70790	ASY2367	Intron 1	21742 to 21871	21742 to 21871	Chr15:38056684-38056555	5	208	0.993	250
	4-plex	Grik2	Glur6	14806	ASY2317	5'-Upstream, 5'-UTR	-5608 to -5466	-101 to 42	Chr10:49788867-49788725	20	194	0.95	100
	Ta = 59	Traf1	Ebi6	22029	ASY2352	Intron 2	-359 to -322	3147 to 3184	Chr2:34958439-34958402	3	106	0.976	300
		Ubr5	Edd1	70790	ASY2357	5'-Upstream	-1180 to -1168	-1180 to -1168	Chr15:38079605-38079593	3	88	0.82	400
	N129P7	Timp3	K222	21859	ASY2421	5'-Upstream	-4655 to -4545	-4173 to -4063	Chr10:86296199-86296309	8	181	0.966	4800
	9-plex	Cttn	Ems1	13043	ASY2441	Intron 2	-1495 to -1458	5517 to 5554	Chr7:144465493-144465456	2	140	0.941	250
	Ta = 59	Msn	C78546	17698	ASY1546	5'-Upstream	-597 to -537	-407 to -347	ChrX:96095635-96095695	2	135	0.991	200
		Pax3	Hup2	18505	ASY2449	5'-Upstream	-863 to -799	-774 to -710	Chr1:78197612-78197548	5	131	0.963	100
		Pax3	Hup2	18505	ASY2447	5'-Upstream	-1429 to -1381	-1340 to -1292	Chr1:78198178-78198130	4	123	0.926	300
		Wnt9a	Wnt14	216795	ASY2430	5'-Upstream	-1625 to -1569	-1582 to -1526	Chr11:59305348-59305404	4	122	0.804	350
		Pax3	Hup2	18505	ASY2446	5'-Upstream	-2424 to -2368	-2335 to -2279	Chr1:78199173-78199117	3	115	0.928	1600
		Pax3	Hup2	18505	ASY2452	Intron 1	727 to 772	816 to 861	Chr1:78196023-78195978	6	106	0.929	4000
		Pax3	Hup2	18505	ASY2445	5'-Upstream	-2581 to -2529	-2492 to -2440	Chr1:78199330-78199278	4	105	0.865	900
	N129P8	Timp3	K222	21859	ASY2427	Exon 1, Intron 1	60 to 154	542 to 636	Chr10:86300913-86301007	7	163	0.949	1500
	8-plex	Cttn	Ems1	13043	ASY2439	Intron 1	-6457 to -6372	555 to 640	Chr7:144470455-144470370	9	163	0.985	1000
	Ta = 62	Pax3	Hup2	18505	ASY2450	Intron 1	105 to 193	194 to 282	Chr1:78196645-78196557	7	153	0.95	2000
		Msn	C78546	17698	ASY1547	5'-Upstream	-446 to -432	-256 to -242	ChrX:96095786-96095800	2	116	0.99	10000
		Msn	C78546	17698	ASY1558	Intron 1	938 to 973	1128 to 1163	ChrX:96097169-96097204	2	115	0.991	400
		Gsk3b	Gsk	56637	ASY2032	Exon 1	5 to 32	1530 to 1557	Chr16:38090530-38090557	4	107	0.919	9500
		Msn	C78546	17698	ASY1557	Intron 1	828 to 863	1018 to 1053	ChrX:96097059-96097094	2	105	0.993	1200
		Cttn	Ems1	13043	ASY2436	5'-UTR	-6924 to -6884	88 to 128	Chr7:144470922-144470882	5	100	0.934	2000
	N129P9	Wnt9a	Wnt14	216795	ASY2435	Intron 1	1229 to 1330	1272 to 1373	Chr11:59308201-59308302	6	156	0.849	7500
	2-plex Ta = 59	Pax3	Hup2	18505	ASY2444	5'-Upstream	-2795 to -2700	-2706 to -2611	Chr1:78199544-78199449	4	141	0.948	1500
	N129P10	Gsk3b	Gsk	56637	ASY2031	5'-Upstream	-2362 to -2217	-837 to -692	Chr16:38088164-38088309	10	240	0.936	500
	7-plex	Cttn	Ems1	13043	ASY2440	Intron 1	-4948 to -4867	2064 to 2145	Chr7:144468946-144468865	4	179	0.983	200
	Ta = 57	Cttn	Ems1	13043	ASY2438	Intron 1	-6616 to -6504	396 to 508	Chr7:144470614-144470502	7	178	0.967	2000
		Pax3	Hup2	18505	ASY2451	Intron 1	238 to 357	327 to 446	Chr1:78196512-78196393	7	163	0.873	2400
		Msn	C78546	17698	ASY1556	Intron 1	169 to 246	359 to 436	ChrX:96096400-96096477	3	152	0.99	3800
		Gsk3b	Gsk	56637	ASY2033	Intron 1	347 to 417	1872 to 1942	Chr16:38090872-38090942	6	146	0.872	100
		Wnt9a	Wnt14	216795	ASY2433	5'-Upstream	-433 to -386	-390 to -343	Chr11:59306540-59306587	5	117	0.85	450
	N129P11	Msn	C78546	17698	ASY1560	Intron 2	48785 to 48939	48975 to 49129	ChrX:96145016-96145170	3	221	0.991	400
	7-plex	Cttn	Ems1	13043	ASY2437	5'-UTR	-6824 to -6718	188 to 294	Chr7:144470822-144470716	17	188	0.934	100
	Ta = 59	Wnt9a	Wnt14	216795	ASY2434	Intron 1	946 to 1076	989 to 1119	Chr11:59307918-59308048	12	184	0.746	150
		Msn	C78546	17698	ASY1559	Intron 1	1113 to 1207	1303 to 1397	ChrX:96097344-96097438	2	170	0.995	300
		Pax3	Hup2	18505	ASY2442	5'-Upstream	-3962 to -3856	-3873 to -3767	Chr1:78200711-78200605	8	170	0.833	10000
		Wnt9a	Wnt14	216795	ASY2431	5'-Upstream	-1143 to -1032	-1100 to -989	Chr11:59305830-59305941	5	163	0.838	10000
		Pax3	Hup2	18505	ASY2453	Exon 2	1380 to 1424	1469 to 1513	Chr1:78195370-78195326	6	115	0.971	500
	11 PCRs	13 genes			77 assays					396 CpGs	11,304 bp

NGS096/095 - Mouse Rxra & Vdr (retinoid X receptor alpha & vitamin D receptor)	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N95P2	Rxra	Nr2b1	20181	ADS9215	Intron 1	47789 to 47915	48021 to 48147	Chr2:27724460-27724586	5	192	0.841	50
	6-plex	Rxra	Nr2b1	20181	ADS9219	Intron 2	62996 to 63097	63228 to 63329	Chr2:27739667-27739768	7	162	0.777	50
	Ta = 62	Rxra	Nr2b1	20181	ADS9159	Intron 1	6778 to 6837	7010 to 7069	Chr2:27683449-27683508	3	126	0.802	600
		Rxra	Nr2b1	20181	ADS9099	Intron 1	2452 to 2461	2684 to 2693	Chr2:27679123-27679132	2	110	0.743	350
		Rxra	Nr2b1	20181	ADS9229	Intron 1	13398 to 13425	13630 to 13657	Chr2:27690069-27690096	2	110	0.881	300
		Rxra	Nr2b1	20181	ADS9227	3'-Downstream	86629 to 86648	86861 to 86880	Chr2:27763300-27763319	3	109	0.852	50
	N95P3	Rxra	Nr2b1	20181	ADS9214	Intron 1	21597 to 21756	21829 to 21988	Chr2:27698268-27698427	6	212	0.974	50
	6-plex	Rxra	Nr2b1	20181	ADS9040	Intron 1	1767 to 1832	1999 to 2064	Chr2:27678438-27678503	2	162	0.578	50
	Ta = 62	Rxra	Nr2b1	20181	ADS9228	Intron 1	11366 to 11437	11598 to 11669	Chr2:27688037-27688108	3	142	0.874	150
		Rxra	Nr2b1	20181	ADS9212	Intron 1	17560 to 17629	17792 to 17861	Chr2:27694231-27694300	5	138	0.84	400
		Rxra	Nr2b1	20181	ADS9216	Intron 1	51164 to 51194	51396 to 51426	Chr2:27727835-27727865	5	95	0.724	1000
		Rxra	Nr2b1	20181	ADS9224	Intron 5	71722 to 71747	71954 to 71979	Chr2:27748393-27748418	3	82	0.824	150
	N96P2	Vdr	Nr1i1	22337	ADS9232	Intron 1	-15032 to -14972	8340 to 8400	Chr15:97899971-97899911	3	127	0.922	1000
	6-plex	Vdr	Nr1i1	22337	ADS9239	Intron 3	7092 to 7145	30463 to 30516	Chr15:97877848-97877795	3	125	0.965	250
	Ta = 59	Vdr	Nr1i1	22337	ADS9236	Intron 1	-4177 to -4117	19195 to 19255	Chr15:97889116-97889056	3	122	0.996	200
		Vdr	Nr1i1	22337	ADS9240	Intron 4	13667 to 13678	37038 to 37049	Chr15:97871273-97871262	2	122	0.95	500
		Vdr	Nr1i1	22337	ADS9243	Intron 7	23530 to 23573	46901 to 46944	Chr15:97861410-97861367	3	120	0.848	1200
		Vdr	Nr1i1	22337	ADS9237	Intron 3	4082 to 4140	27453 to 27511	Chr15:97880858-97880800	4	112	0.927	1000
	N96P3	Vdr	Nr1i1	22337	ADS9241	Intron 7	19603 to 19672	42974 to 43043	Chr15:97865337-97865268	4	175	0.834	70
	6-plex	Vdr	Nr1i1	22337	ADS9231	Intron 1	-19446	3926	Chr15:97904385	6	164	0.855	150
	Ta = 59	Vdr	Nr1i1	22337	ADS9244	3'-Downstream	35299 to 35403	58670 to 58774	Chr15:97849641-97849537	10	161	0.689	50
		Vdr	Nr1i1	22337	ADS9234	Intron 1	-7711 to -7644	15661 to 15728	Chr15:97892650-97892583	4	146	0.922	300
		Vdr	Nr1i1	22337	ADS9238	Intron 3	6134 to 6200	29505 to 29571	Chr15:97878806-97878740	3	144	0.938	700
		Vdr	Nr1i1	22337	ADS9233	Intron 1	-11184 to -11128	12188 to 12244	Chr15:97896123-97896067	5	134	0.977	400
	N96P4	Vdr	Nr1i1	22337	ADS9235	Intron 3	1526 to 1565	24897 to 24936	Chr15:97883414-97883375	3	109	0.887	600
	3-plex	Vdr	Nr1i1	22337	ADS9242	Intron 7	20960 to 20997	44331 to 44368	Chr15:97863980-97863943	4	100	0.865	300
	Ta = 53	Vdr	Nr1i1	22337	ADS9230	Intron 1	-22311 to -22303	1061 to 1069	Chr15:97907250-97907242	2	88	0.881	150
	5 PCRs	2 genes			27 assays					105 CpGs	3,589 bp

NGS074 - Mouse Cdkn2a & Clca1	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N74P1	Cdkn2a	p16	12578	ADS5218	Exon 1	37 to 126	167 to 256	Chr4:89294487-89294398	10	159	0.9576	800
	4-plex	Cdkn2a	p16	12578	ADS5226	Intron 1	12884 to 12941	13014 to 13071	Chr4:89281640-89281583	2	153	0.9005	4000
	Ta = 59	Cdkn2a	p16	12578	ADS5228	Intron 1	13231 to 13281	13361 to 13411	Chr4:89281293-89281243	2	105	0.9828	2400
		Cdkn2a	p16	12578	ADS5224	Intron 1	7509 to 7532	7639 to 7662	Chr4:89287015-89286992	3	95	0.9859	4500
	N74P2	Cdkn2a	p16	12578	ADS5217	5'-Upstream, 5'-UTR, Exon 1	-131 to 7	-1 to 137	Chr4:89294654-89294517	15	228	0.9729	900
	5-plex	Cdkn2a	p16	12578	ADS5231	Intron 2	19440 to 19583	19570 to 19713	Chr4:89275084-89274941	9	204	0.9599	150
	Ta = 56	Cdkn2a	p16	12578	ADS5225	Intron 1	11946 to 12019	12076 to 12149	Chr4:89282578-89282505	4	131	0.9609	2200
		Cdkn2a	p16	12578	ADS5223	Intron 1	5579 to 5609	5709 to 5739	Chr4:89288945-89288915	2	111	0.9852	1700
		Cdkn2a	p16	12578	ADS5221	Intron 1	1287	1417	Chr4:89293237	1	75	0.978	500
	N74P3	Clca1	Gob5	23844	ADS5256	Exon 1, Intron 1	90 to 264	121 to 295	Chr3:145032656-145032482	7	260	0.9612	200
	8-plex	Clca1	Gob5	23844	ADS5252	Intron 1	3512 to 3634	3543 to 3665	Chr3:145029234-145029112	3	195	0.9809	1000
	Ta = 55	Clca1	Gob5	23844	ADS5244	Exon 14	27676 to 27741	27707 to 27772	Chr3:145005070-145005005	3	186	0.9697	100
		Clca1	Gob5	23844	ADS5242	3'-UTR	28128 to 28143	28159 to 28174	Chr3:145004618-145004603	2	154	0.9798	2000
		Clca1	Gob5	23844	ADS5258	5'-Upstream	-2476	-2445	Chr3:145035221	1	144	0.9958	700
		Clca1	Gob5	23844	ADS5248	Exon 13, Intron 13	26828 to 26896	26859 to 26927	Chr3:145005918-145005850	6	125	0.9822	3000
		Clca1	Gob5	23844	ADS5250	Exon 3	5357	5388	Chr3:145027389	1	124	0.9891	1800
		Clca1	Gob5	23844	ADS5246	3'-UTR	28212	28243	Chr3:145004534	1	96	0.9761	2900
	N74P4	Clca1	Gob5	23844	ADS5259	5'-Upstream	-4720 to -4576	-4689 to -4545	Chr3:145037465-145037321	8	234	0.9828	200
	7-plex	Clca1	Gob5	23844	ADS5251	Intron 1, Exon 2	4683 to 4818	4714 to 4849	Chr3:145028063-145027928	3	216	0.9294	700
	Ta = 55	Clca1	Gob5	23844	ADS5243	Exon 14	27886 to 27987	27917 to 28018	Chr3:145004860-145004759	5	189	0.987	400
		Clca1	Gob5	23844	ADS5247	3'-Downstream	30730 to 30805	30761 to 30836	Chr3:145002016-145001941	3	179	0.9831	200
		Clca1	Gob5	23844	ADS5253	Intron 1	1480 to 1549	1511 to 1580	Chr3:145031266-145031197	2	159	0.9831	1000
		Clca1	Gob5	23844	ADS5245	Intron 13	27563	27594	Chr3:145005183	1	145	0.9843	1800
		Clca1	Gob5	23844	ADS5257	5'-Upstream	-631 to -588	-600 to -557	Chr3:145033376-145033333	2	118	0.9801	2800
	Simplex Ta = 59	Cdkn2a	p16	12578	ADS5214	5'-Upstream	-1242 to -1085	-1112 to -955	Chr4:89295765-89295608	9	223	0.9087	2500
	Simplex Ta = 56	Cdkn2a	p16	12578	ADS5215	5'-Upstream	-636 to -522	-506 to -392	Chr4:89295159-89295045	17	184	0.8463	1200
	6 PCRs	2 genes			26 assays					122 CpGs	4,192 bp

NGS004V1 - Mouse Foxp3 (forkhead box P3) Regulators	PCR ID	Gene	Alias	NCBI ID	Assay ID	Location	From ATG	From TSS	Coordinates (mm10)	# CpGs	Size (bp)	RSQ	Est. Cov.
	N4V1P15	Ikzf4	Eos	22781	ADS3785	Intron 1	1554 to 1662	1738 to 1846	Chr10:128644254-128644146	5	210	0.971	700
	6-plex	Il2	Tcgf	16183	ASY548	5'-Upstream	-308 to -263	-255 to -210	Chr3:37126214-37126169	2	188	0.988	3500
	Ta = 58	Foxp3	Jm2	20371	ADS443	Intron 1	-2405 to -2287	4275 to 4393	ChrX:7583950-7584068	6	181	0.951	300
		Ikzf2	Helios	22779	ADS4020	Intron 3	26884 to 26961	28548 to 28625	Chr1:69657274-69657197	6	147	0.991	8000
		Foxp3	Jm2	20371	ADS779B	Intron 1	-2145 to -2075	4535 to 4605	ChrX:7584210-7584280	2	144	0.981	6000
		Foxp3	Jm2	20371	ADS569B	5'-Upstream	-12069 to -12010	-5390 to -5331	ChrX:7574286-7574345	15	113	0.947	800
	N4V1P16	Tnfrsf18	Gitr	21936	ADS4018	Exon 5, 3'-UTR	2180 to 2262	2403 to 2485	Chr4:156028566-156028648	4	210	0.973	100
	6-plex	Ctla4	Cd152	12477	ADS3784B	Exon 2	3302 to 3403	3448 to 3549	Chr1:60912472-60912573	4	161	0.987	700
	Ta = 55	Tnf	Tnfa	21926	ASY2219	5'-Upstream	-1006 to -910	-839 to -743	Chr17:35202846-35202750	4	144	0.9	2000
		Foxp3	Jm2	20371	ADS442	Intron 1	-2555 to -2533	4125 to 4147	ChrX:7583800-7583822	2	129	0.975	300
		Foxp3	Jm2	20371	ADS779A	Intron 1	-2238 to -2207	4442 to 4473	ChrX:7584117-7584148	4	111	0.963	100
		Foxp3	Jm2	20371	ASY1724	5'-Upstream	-12859 to -12811	-6180 to -6132	ChrX:7573496-7573544	9	104	0.994	2000
	N4V1P17	Cd274	Pdl1	60533	ADS8832A	5'-Upstream	-5104 to -5025	-91 to -12	Chr19:29367364-29367443	7	149	0.952	1500
	5-plex	Il2ra	Cd25	16184	ADS4019	Intron 1	2705 to 2738	2899 to 2932	Chr2:11645705-11645738	3	144	0.949	1200
	Ta = 52	Il4	Bsf-1	16189	ADS017B	Intron 1	186 to 230	249 to 293	Chr11:53618421-53618377	3	129	0.985	7000
		Cdkn1c	p57	12577	ADS4569	5'-UTR	-350 to -275	33 to 108	Chr7:143461018-143460943	9	123	0.755	3000
		Foxp3	Jm2	20371	ADS1183	5'-Upstream	-6750 to -6714	-71 to -35	ChrX:7579605-7579641	5	105	0.978	2500
	3 PCRs	11 genes			17 assays					90 CpGs	2,492 bp

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