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Gene Specific DNA Methylation

Gene Specific DNA Methylation

Service Description

Analysis of the percentage methylation levels of all consecutive CpG sites within a gene locus.

Since DNA methylation is frequently heterogeneous within a sample, it is important to quantify the relative proportion of methylated and unmethylated alleles at each CpG site. We achieve this by direct Pyrosequencing after bisulfite modification and PCR amplification.

All of our assays are pre-validated to ensure there is no preferential amplification for either methylated or unmethylated DNA, allowing us to provide the highest quality data and results. EpigenDx has almost 15 years of expertise in epigenetics using Pyrosequencing technology.

Gene Specific DNA Methylation

How It Works

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Tell Us About Your Project

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Send Us Your Sample

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Receive Your Data

Step One | Selecting a Pyrosequencing Target

Figure 1: Promoter Region of Human GCR (NR3C1) (Ensembl Transcript: ENST00000231509)

epigendx-gcrcpg_Human GCR NR3C1 chart

Figure 1: This is an example of EpigenDx’s CpG site coverage in a typical gene such as Human GCR. Red markers are CpG sites covered by at least one of EpigenDx’s pre-developed assays (ADS). Blue X’s are all other CpG sites.

Step Two | Choose an Ideal Plan for You

All parts of our methylation services are customizable and modular. Pick and choose the service plan that is appropriate and ideal for your needs.

Items Service Products Description Order
8001 DNA Methylation Analysis – Standard Quantitative Methylation Analysis on submitted bisulfite-converted PCR products

  • Perform Pyrosequencing run & methylation analysis ONLY
Inquiry / Order
8002 DNA Methylation Analysis – Gold Quantitative Methylation Analysis on submitted bisulfite modified DNA

  • Perform PCR reactions
  • Perform Pyrosequencing methylation analysis
Inquiry / Order
8003 DNA Methylation Analysis – Premium Quantitative Methylation Analysis on submitted genomic DNA

  • Perform bisulfite modification
  • Perform PCR reactions
  • Perform Pyrosequencing analysis
Inquiry / Order

Turn-around time is 5-15 days for most DNA methylation services, depending the number of assays and samples sent. Click here for a detailed breakdown of EpigenDx’s methylation service workflow.

Step Three | Results

EpigenDx analyzes the raw data and delivers:

    • The percentage methylation values of each CpG site in a Microsoft Excel spreadsheet.
    • The raw pyrograms in a PDF document.

Interpretation of Data

Methylation data can then be applied to research. For example, normal healthy individuals maintain high methylation levels (75-85%) in the LINE-1 elements to suppress retrotransposons that would otherwise copy and transpose themselves erratically across the genome. Abnormally low methylation in the LINE elements have been shown to be common in many malignancies, due to genomic instability.

Turn-around time is 5-15 days for most DNA methylation services, depending the number of assays and samples sent. Click here for a detailed breakdown of EpigenDx’s methylation service workflow.

epigendx_methylation of ovarian tissue_cmyc2_chart

Now Offering GLP and GCP Compliant Laboratory Services

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EpigenDx Offers Many Customized Services
YOU DON'T WANT TO MISS THIS!Epigenetics In The A.M. 2021 Event
Are you interested in DNA methylation, gene expression & gene regulation? Then this online event is for you! Let’s share our expertise on how to generate meaningful methylation data for publications, clinical research, biomarker discoveries & much more.

Two lucky winners will win the Raffle Giveaways!